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One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications

Carbon-based photosensitizers are more attractive than the other ones based on their low cost, high stability, broadband of light absorption, tunable emission spectra, high quantum yield, water solubility, high resistance to metabolic degradation, and selective delivery. These properties allow multi...

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Autores principales: Romero, María Paulina, Alves, Fernanda, Stringasci, Mirian Denise, Buzzá, Hilde Harb, Ciol, Heloísa, Inada, Natalia Mayumi, Bagnato, Vanderlei Salvador
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255795/
https://www.ncbi.nlm.nih.gov/pubmed/34234756
http://dx.doi.org/10.3389/fmicb.2021.662149
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author Romero, María Paulina
Alves, Fernanda
Stringasci, Mirian Denise
Buzzá, Hilde Harb
Ciol, Heloísa
Inada, Natalia Mayumi
Bagnato, Vanderlei Salvador
author_facet Romero, María Paulina
Alves, Fernanda
Stringasci, Mirian Denise
Buzzá, Hilde Harb
Ciol, Heloísa
Inada, Natalia Mayumi
Bagnato, Vanderlei Salvador
author_sort Romero, María Paulina
collection PubMed
description Carbon-based photosensitizers are more attractive than the other ones based on their low cost, high stability, broadband of light absorption, tunable emission spectra, high quantum yield, water solubility, high resistance to metabolic degradation, and selective delivery. These properties allow multiple applications in the field of biology and medicine. The present study evaluated in vitro and in vivo the antimicrobial photodynamic effect of a one-pot microwave produced C-DOTS based on citric acid. The in vitro assays assessed the effectiveness of illuminated C-DOTS (C-DOTS + light) against Staphylococcus aureus suspension and biofilm. The concentrations of 6.9 and 13.8 mg/mL of C-DOTS and light doses of 20 and 40 J/cm(2) were able to reduce significantly the microorganisms. Based on these parameters and results, the in vivo experiments were conducted in mice, evaluating this treatment on wounds contaminated with S. aureus. The viability test showed that C-DOTS–mediated photodynamic inactivation reduced 10(4) log of the bacteria present on the skin lesions. These results, altogether, showed that antibacterial photodynamic therapy using C-DOTS is a promising and viable treatment for Gram-positive bacteria-infected wounds.
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spelling pubmed-82557952021-07-06 One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications Romero, María Paulina Alves, Fernanda Stringasci, Mirian Denise Buzzá, Hilde Harb Ciol, Heloísa Inada, Natalia Mayumi Bagnato, Vanderlei Salvador Front Microbiol Microbiology Carbon-based photosensitizers are more attractive than the other ones based on their low cost, high stability, broadband of light absorption, tunable emission spectra, high quantum yield, water solubility, high resistance to metabolic degradation, and selective delivery. These properties allow multiple applications in the field of biology and medicine. The present study evaluated in vitro and in vivo the antimicrobial photodynamic effect of a one-pot microwave produced C-DOTS based on citric acid. The in vitro assays assessed the effectiveness of illuminated C-DOTS (C-DOTS + light) against Staphylococcus aureus suspension and biofilm. The concentrations of 6.9 and 13.8 mg/mL of C-DOTS and light doses of 20 and 40 J/cm(2) were able to reduce significantly the microorganisms. Based on these parameters and results, the in vivo experiments were conducted in mice, evaluating this treatment on wounds contaminated with S. aureus. The viability test showed that C-DOTS–mediated photodynamic inactivation reduced 10(4) log of the bacteria present on the skin lesions. These results, altogether, showed that antibacterial photodynamic therapy using C-DOTS is a promising and viable treatment for Gram-positive bacteria-infected wounds. Frontiers Media S.A. 2021-06-21 /pmc/articles/PMC8255795/ /pubmed/34234756 http://dx.doi.org/10.3389/fmicb.2021.662149 Text en Copyright © 2021 Romero, Alves, Stringasci, Buzzá, Ciol, Inada and Bagnato. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Romero, María Paulina
Alves, Fernanda
Stringasci, Mirian Denise
Buzzá, Hilde Harb
Ciol, Heloísa
Inada, Natalia Mayumi
Bagnato, Vanderlei Salvador
One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications
title One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications
title_full One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications
title_fullStr One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications
title_full_unstemmed One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications
title_short One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications
title_sort one-pot microwave-assisted synthesis of carbon dots and in vivo and in vitro antimicrobial photodynamic applications
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255795/
https://www.ncbi.nlm.nih.gov/pubmed/34234756
http://dx.doi.org/10.3389/fmicb.2021.662149
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