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Protocol for in vitro BCR-mediated plasma cell differentiation and purification of chromatin-associated proteins

Molecular-level understanding of plasma cell (PC) differentiation has been modeled using lipopolysaccharide (LPS) stimulation in vitro. However, this system does not involve the B-cell receptor (BCR)—a critical component of B cell biology. Here, we present a protocol for in vitro PC differentiation...

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Detalles Bibliográficos
Autores principales: Ochiai, Kyoko, Shima, Hiroki, Ikura, Tsuyoshi, Franke, Marissa C., Sievert, Evelyn P., Sciammas, Roger, Igarashi, Kazuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255920/
https://www.ncbi.nlm.nih.gov/pubmed/34258594
http://dx.doi.org/10.1016/j.xpro.2021.100633