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Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites

Maize (Zea mays subsp. mays) yield loss from arthropod herbivory is substantial. While the basis of resistance to major insect herbivores has been comparatively well-studied in maize, less is known about resistance to spider mite herbivores, which are distantly related to insects and feed by a diffe...

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Autores principales: Bui, Huyen, Greenhalgh, Robert, Gill, Gunbharpur S., Ji, Meiyuan, Kurlovs, Andre H., Ronnow, Christian, Lee, Sarah, Ramirez, Ricardo A., Clark, Richard M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256171/
https://www.ncbi.nlm.nih.gov/pubmed/34234802
http://dx.doi.org/10.3389/fpls.2021.693088
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author Bui, Huyen
Greenhalgh, Robert
Gill, Gunbharpur S.
Ji, Meiyuan
Kurlovs, Andre H.
Ronnow, Christian
Lee, Sarah
Ramirez, Ricardo A.
Clark, Richard M.
author_facet Bui, Huyen
Greenhalgh, Robert
Gill, Gunbharpur S.
Ji, Meiyuan
Kurlovs, Andre H.
Ronnow, Christian
Lee, Sarah
Ramirez, Ricardo A.
Clark, Richard M.
author_sort Bui, Huyen
collection PubMed
description Maize (Zea mays subsp. mays) yield loss from arthropod herbivory is substantial. While the basis of resistance to major insect herbivores has been comparatively well-studied in maize, less is known about resistance to spider mite herbivores, which are distantly related to insects and feed by a different mechanism. Two spider mites, the generalist Tetranychus urticae, and the grass-specialist Oligonychus pratensis, are notable pests of maize, especially during drought conditions. We assessed resistance (antibiosis) to both mites of 38 highly diverse maize lines, including several previously reported to be resistant to one or the other mite species. We found that line B96, as well as its derivatives B49 and B75, were highly resistant to T. urticae. In contrast, neither these three lines, nor any others included in our study, were notably resistant to the specialist O. pratensis. Quantitative trait locus (QTL) mapping with replicate populations from crosses of B49, B75, and B96 to susceptible B73 identified a QTL in the same genomic interval on chromosome 6 for T. urticae resistance in each of the three resistant lines, and an additional resistance QTL on chromosome 1 was unique to B96. Single-locus genotyping with a marker coincident with the chromosome 6 QTL in crosses of both B49 and B75 to B73 revealed that the respective QTL was large-effect; it explained ∼70% of the variance in resistance, and resistance alleles from B49 and B75 acted recessively as compared to B73. Finally, a genome-wide haplotype analysis using genome sequence data generated for B49, B75, and B96 identified an identical haplotype, likely of initial origin from B96, as the source of T. urticae resistance on chromosome 6 in each of the B49, B75, and B96 lines. Our findings uncover the relationship between intraspecific variation in maize defenses and resistance to its major generalist and specialist spider mite herbivores, and we identified loci for use in breeding programs and for genetic studies of resistance to T. urticae, the most widespread spider mite pest of maize.
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spelling pubmed-82561712021-07-06 Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites Bui, Huyen Greenhalgh, Robert Gill, Gunbharpur S. Ji, Meiyuan Kurlovs, Andre H. Ronnow, Christian Lee, Sarah Ramirez, Ricardo A. Clark, Richard M. Front Plant Sci Plant Science Maize (Zea mays subsp. mays) yield loss from arthropod herbivory is substantial. While the basis of resistance to major insect herbivores has been comparatively well-studied in maize, less is known about resistance to spider mite herbivores, which are distantly related to insects and feed by a different mechanism. Two spider mites, the generalist Tetranychus urticae, and the grass-specialist Oligonychus pratensis, are notable pests of maize, especially during drought conditions. We assessed resistance (antibiosis) to both mites of 38 highly diverse maize lines, including several previously reported to be resistant to one or the other mite species. We found that line B96, as well as its derivatives B49 and B75, were highly resistant to T. urticae. In contrast, neither these three lines, nor any others included in our study, were notably resistant to the specialist O. pratensis. Quantitative trait locus (QTL) mapping with replicate populations from crosses of B49, B75, and B96 to susceptible B73 identified a QTL in the same genomic interval on chromosome 6 for T. urticae resistance in each of the three resistant lines, and an additional resistance QTL on chromosome 1 was unique to B96. Single-locus genotyping with a marker coincident with the chromosome 6 QTL in crosses of both B49 and B75 to B73 revealed that the respective QTL was large-effect; it explained ∼70% of the variance in resistance, and resistance alleles from B49 and B75 acted recessively as compared to B73. Finally, a genome-wide haplotype analysis using genome sequence data generated for B49, B75, and B96 identified an identical haplotype, likely of initial origin from B96, as the source of T. urticae resistance on chromosome 6 in each of the B49, B75, and B96 lines. Our findings uncover the relationship between intraspecific variation in maize defenses and resistance to its major generalist and specialist spider mite herbivores, and we identified loci for use in breeding programs and for genetic studies of resistance to T. urticae, the most widespread spider mite pest of maize. Frontiers Media S.A. 2021-06-21 /pmc/articles/PMC8256171/ /pubmed/34234802 http://dx.doi.org/10.3389/fpls.2021.693088 Text en Copyright © 2021 Bui, Greenhalgh, Gill, Ji, Kurlovs, Ronnow, Lee, Ramirez and Clark. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bui, Huyen
Greenhalgh, Robert
Gill, Gunbharpur S.
Ji, Meiyuan
Kurlovs, Andre H.
Ronnow, Christian
Lee, Sarah
Ramirez, Ricardo A.
Clark, Richard M.
Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites
title Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites
title_full Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites
title_fullStr Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites
title_full_unstemmed Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites
title_short Maize Inbred Line B96 Is the Source of Large-Effect Loci for Resistance to Generalist but Not Specialist Spider Mites
title_sort maize inbred line b96 is the source of large-effect loci for resistance to generalist but not specialist spider mites
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256171/
https://www.ncbi.nlm.nih.gov/pubmed/34234802
http://dx.doi.org/10.3389/fpls.2021.693088
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