Cargando…
Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations
Monochamus saltuarius (Coleoptera: Cerambycidae) is an important native pest in the pine forests of northeast China and a dispersing vector of an invasive species Bursaphelenchus xylophilus. To investigate the bacterial gut diversity of M. saltuarius larvae in different host species, and infer the r...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256174/ https://www.ncbi.nlm.nih.gov/pubmed/34234761 http://dx.doi.org/10.3389/fmicb.2021.687211 |
_version_ | 1783718047504138240 |
---|---|
author | Ge, Si-Xun Shi, Feng-Ming Pei, Jia-He Hou, Ze-Hai Zong, Shi-Xiang Ren, Li-Li |
author_facet | Ge, Si-Xun Shi, Feng-Ming Pei, Jia-He Hou, Ze-Hai Zong, Shi-Xiang Ren, Li-Li |
author_sort | Ge, Si-Xun |
collection | PubMed |
description | Monochamus saltuarius (Coleoptera: Cerambycidae) is an important native pest in the pine forests of northeast China and a dispersing vector of an invasive species Bursaphelenchus xylophilus. To investigate the bacterial gut diversity of M. saltuarius larvae in different host species, and infer the role of symbiotic bacteria in host adaptation, we used 16S rRNA gene Illumina sequencing and liquid chromatography-mass spectrometry metabolomics processing to obtain and compare the composition of the bacterial community and metabolites in the midguts of larvae feeding on three host tree species: Pinus koraiensis, Pinus sylvestris var. mongolica, and Pinus tabuliformis. Metabolomics in xylem samples from the three aforementioned hosts were also performed. Proteobacteria and Firmicutes were the predominant bacterial phyla in the larval gut. At the genus level, Klebsiella, unclassified_f__Enterobacteriaceae, Lactococcus, and Burkholderia–Caballeronia–Paraburkholderia were most dominant in P. koraiensis and P. sylvestris var. mongolica feeders, while Burkholderia–Caballeronia–Paraburkholderia, Dyella, Pseudoxanthomonas, and Mycobacterium were most dominant in P. tabuliformis feeders. Bacterial communities were similar in diversity in P. koraiensis and P. sylvestris var. mongolica feeders, while communities were highly diverse in P. tabuliformis feeders. Compared with the other two tree species, P. tabuliformis xylems had more diverse and abundant secondary metabolites, while larvae feeding on these trees had a stronger metabolic capacity for secondary metabolites than the other two host feeders. Correlation analysis of the association of microorganisms with metabolic features showed that dominant bacterial genera in P. tabuliformis feeders were more negatively correlated with plant secondary metabolites than those of other host tree feeders. |
format | Online Article Text |
id | pubmed-8256174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82561742021-07-06 Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations Ge, Si-Xun Shi, Feng-Ming Pei, Jia-He Hou, Ze-Hai Zong, Shi-Xiang Ren, Li-Li Front Microbiol Microbiology Monochamus saltuarius (Coleoptera: Cerambycidae) is an important native pest in the pine forests of northeast China and a dispersing vector of an invasive species Bursaphelenchus xylophilus. To investigate the bacterial gut diversity of M. saltuarius larvae in different host species, and infer the role of symbiotic bacteria in host adaptation, we used 16S rRNA gene Illumina sequencing and liquid chromatography-mass spectrometry metabolomics processing to obtain and compare the composition of the bacterial community and metabolites in the midguts of larvae feeding on three host tree species: Pinus koraiensis, Pinus sylvestris var. mongolica, and Pinus tabuliformis. Metabolomics in xylem samples from the three aforementioned hosts were also performed. Proteobacteria and Firmicutes were the predominant bacterial phyla in the larval gut. At the genus level, Klebsiella, unclassified_f__Enterobacteriaceae, Lactococcus, and Burkholderia–Caballeronia–Paraburkholderia were most dominant in P. koraiensis and P. sylvestris var. mongolica feeders, while Burkholderia–Caballeronia–Paraburkholderia, Dyella, Pseudoxanthomonas, and Mycobacterium were most dominant in P. tabuliformis feeders. Bacterial communities were similar in diversity in P. koraiensis and P. sylvestris var. mongolica feeders, while communities were highly diverse in P. tabuliformis feeders. Compared with the other two tree species, P. tabuliformis xylems had more diverse and abundant secondary metabolites, while larvae feeding on these trees had a stronger metabolic capacity for secondary metabolites than the other two host feeders. Correlation analysis of the association of microorganisms with metabolic features showed that dominant bacterial genera in P. tabuliformis feeders were more negatively correlated with plant secondary metabolites than those of other host tree feeders. Frontiers Media S.A. 2021-06-21 /pmc/articles/PMC8256174/ /pubmed/34234761 http://dx.doi.org/10.3389/fmicb.2021.687211 Text en Copyright © 2021 Ge, Shi, Pei, Hou, Zong and Ren. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Ge, Si-Xun Shi, Feng-Ming Pei, Jia-He Hou, Ze-Hai Zong, Shi-Xiang Ren, Li-Li Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations |
title | Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations |
title_full | Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations |
title_fullStr | Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations |
title_full_unstemmed | Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations |
title_short | Gut Bacteria Associated With Monochamus saltuarius (Coleoptera: Cerambycidae) and Their Possible Roles in Host Plant Adaptations |
title_sort | gut bacteria associated with monochamus saltuarius (coleoptera: cerambycidae) and their possible roles in host plant adaptations |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256174/ https://www.ncbi.nlm.nih.gov/pubmed/34234761 http://dx.doi.org/10.3389/fmicb.2021.687211 |
work_keys_str_mv | AT gesixun gutbacteriaassociatedwithmonochamussaltuariuscoleopteracerambycidaeandtheirpossiblerolesinhostplantadaptations AT shifengming gutbacteriaassociatedwithmonochamussaltuariuscoleopteracerambycidaeandtheirpossiblerolesinhostplantadaptations AT peijiahe gutbacteriaassociatedwithmonochamussaltuariuscoleopteracerambycidaeandtheirpossiblerolesinhostplantadaptations AT houzehai gutbacteriaassociatedwithmonochamussaltuariuscoleopteracerambycidaeandtheirpossiblerolesinhostplantadaptations AT zongshixiang gutbacteriaassociatedwithmonochamussaltuariuscoleopteracerambycidaeandtheirpossiblerolesinhostplantadaptations AT renlili gutbacteriaassociatedwithmonochamussaltuariuscoleopteracerambycidaeandtheirpossiblerolesinhostplantadaptations |