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Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study

INTRODUCTION: Angiotensin-converting-enzyme-2 (ACE-2) was demonstrated to be the receptor for cellular entry of SARS-CoV-2. ACE-2 mRNA was identified in several human tissues and recently also in thyroid cells in vitro. PURPOSE: Aim of the present study was to investigate the effect of pro-inflammat...

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Autores principales: Coperchini, Francesca, Ricci, Gianluca, Croce, Laura, Denegri, Marco, Ruggiero, Rubina, Villani, Laura, Magri, Flavia, Chiovato, Luca, Rotondi, Mario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256224/
https://www.ncbi.nlm.nih.gov/pubmed/34224085
http://dx.doi.org/10.1007/s12020-021-02807-w
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author Coperchini, Francesca
Ricci, Gianluca
Croce, Laura
Denegri, Marco
Ruggiero, Rubina
Villani, Laura
Magri, Flavia
Chiovato, Luca
Rotondi, Mario
author_facet Coperchini, Francesca
Ricci, Gianluca
Croce, Laura
Denegri, Marco
Ruggiero, Rubina
Villani, Laura
Magri, Flavia
Chiovato, Luca
Rotondi, Mario
author_sort Coperchini, Francesca
collection PubMed
description INTRODUCTION: Angiotensin-converting-enzyme-2 (ACE-2) was demonstrated to be the receptor for cellular entry of SARS-CoV-2. ACE-2 mRNA was identified in several human tissues and recently also in thyroid cells in vitro. PURPOSE: Aim of the present study was to investigate the effect of pro-inflammatory cytokines on the ACE-2 mRNA levels in human thyroid cells in primary cultures. METHODS: Primary thyroid cell cultures were treated with IFN-γ and TNF-α alone or in combination for 24 h. ACE-2 mRNA levels were measured by RT-PCR. As a control, the levels of IFN-γ inducible chemokine (CXCL10) were measured in the respective cell culture supernatants. RESULTS: The mean levels of ACE-2 mRNA increased after treatment with IFN-γ and TNF-α in all the thyroid cell preparations, while the combination treatment did not consistently synergically increase ACE-2-mRNA. At difference, CXCL10 was consistently increased by IFN-γ and synergically further increased by the combination treatment with IFN-γ + TNF-α, with respect to IFN-γ alone. CONCLUSIONS: The results of the present study show that IFN-γ and, to a lesser extent TNF-α consistently increase ACE-2 mRNA levels in NHT primary cultures. More interestingly, the combined stimulation (proven to be effective according to the synergic effect registered for CXCL10) produces different responses in terms of ACE-2 mRNA modulation. These results would suggest that elevated levels of pro-inflammatory cytokines could facilitate the entering of the virus in cells by further increasing ACE-2 expression and/or account for the different degree of severity of SARS-COV-2 infection. This hypothesis deserves to be confirmed by further specific studies.
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spelling pubmed-82562242021-07-06 Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study Coperchini, Francesca Ricci, Gianluca Croce, Laura Denegri, Marco Ruggiero, Rubina Villani, Laura Magri, Flavia Chiovato, Luca Rotondi, Mario Endocrine Original Article INTRODUCTION: Angiotensin-converting-enzyme-2 (ACE-2) was demonstrated to be the receptor for cellular entry of SARS-CoV-2. ACE-2 mRNA was identified in several human tissues and recently also in thyroid cells in vitro. PURPOSE: Aim of the present study was to investigate the effect of pro-inflammatory cytokines on the ACE-2 mRNA levels in human thyroid cells in primary cultures. METHODS: Primary thyroid cell cultures were treated with IFN-γ and TNF-α alone or in combination for 24 h. ACE-2 mRNA levels were measured by RT-PCR. As a control, the levels of IFN-γ inducible chemokine (CXCL10) were measured in the respective cell culture supernatants. RESULTS: The mean levels of ACE-2 mRNA increased after treatment with IFN-γ and TNF-α in all the thyroid cell preparations, while the combination treatment did not consistently synergically increase ACE-2-mRNA. At difference, CXCL10 was consistently increased by IFN-γ and synergically further increased by the combination treatment with IFN-γ + TNF-α, with respect to IFN-γ alone. CONCLUSIONS: The results of the present study show that IFN-γ and, to a lesser extent TNF-α consistently increase ACE-2 mRNA levels in NHT primary cultures. More interestingly, the combined stimulation (proven to be effective according to the synergic effect registered for CXCL10) produces different responses in terms of ACE-2 mRNA modulation. These results would suggest that elevated levels of pro-inflammatory cytokines could facilitate the entering of the virus in cells by further increasing ACE-2 expression and/or account for the different degree of severity of SARS-COV-2 infection. This hypothesis deserves to be confirmed by further specific studies. Springer US 2021-07-05 2021 /pmc/articles/PMC8256224/ /pubmed/34224085 http://dx.doi.org/10.1007/s12020-021-02807-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Coperchini, Francesca
Ricci, Gianluca
Croce, Laura
Denegri, Marco
Ruggiero, Rubina
Villani, Laura
Magri, Flavia
Chiovato, Luca
Rotondi, Mario
Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study
title Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study
title_full Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study
title_fullStr Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study
title_full_unstemmed Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study
title_short Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study
title_sort modulation of ace-2 mrna by inflammatory cytokines in human thyroid cells: a pilot study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256224/
https://www.ncbi.nlm.nih.gov/pubmed/34224085
http://dx.doi.org/10.1007/s12020-021-02807-w
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