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Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds
Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norov...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256251/ https://www.ncbi.nlm.nih.gov/pubmed/34187186 http://dx.doi.org/10.1177/20402066211026852 |
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author | Van Dycke, Jana Rymenants, Jasper Neyts, Johan Rocha-Pereira, Joana |
author_facet | Van Dycke, Jana Rymenants, Jasper Neyts, Johan Rocha-Pereira, Joana |
author_sort | Van Dycke, Jana |
collection | PubMed |
description | Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norovirus-targeting small molecules. We have previously established antiviral assays using this system, identified novel inhibitors and performed additional studies in order to have a first insight into their mechanism of action. As potent and safe anti-norovirus small molecules are urgently needed, we here describe the detailed protocol for a set of assays that will allow the identification of novel norovirus inhibitors. |
format | Online Article Text |
id | pubmed-8256251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-82562512021-07-16 Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds Van Dycke, Jana Rymenants, Jasper Neyts, Johan Rocha-Pereira, Joana Antivir Chem Chemother Protocol Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norovirus-targeting small molecules. We have previously established antiviral assays using this system, identified novel inhibitors and performed additional studies in order to have a first insight into their mechanism of action. As potent and safe anti-norovirus small molecules are urgently needed, we here describe the detailed protocol for a set of assays that will allow the identification of novel norovirus inhibitors. SAGE Publications 2021-06-29 /pmc/articles/PMC8256251/ /pubmed/34187186 http://dx.doi.org/10.1177/20402066211026852 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Protocol Van Dycke, Jana Rymenants, Jasper Neyts, Johan Rocha-Pereira, Joana Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds |
title | Assessment of the anti-norovirus activity in cell culture using the
mouse norovirus: Identification of active compounds |
title_full | Assessment of the anti-norovirus activity in cell culture using the
mouse norovirus: Identification of active compounds |
title_fullStr | Assessment of the anti-norovirus activity in cell culture using the
mouse norovirus: Identification of active compounds |
title_full_unstemmed | Assessment of the anti-norovirus activity in cell culture using the
mouse norovirus: Identification of active compounds |
title_short | Assessment of the anti-norovirus activity in cell culture using the
mouse norovirus: Identification of active compounds |
title_sort | assessment of the anti-norovirus activity in cell culture using the
mouse norovirus: identification of active compounds |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256251/ https://www.ncbi.nlm.nih.gov/pubmed/34187186 http://dx.doi.org/10.1177/20402066211026852 |
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