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Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds

Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norov...

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Autores principales: Van Dycke, Jana, Rymenants, Jasper, Neyts, Johan, Rocha-Pereira, Joana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256251/
https://www.ncbi.nlm.nih.gov/pubmed/34187186
http://dx.doi.org/10.1177/20402066211026852
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author Van Dycke, Jana
Rymenants, Jasper
Neyts, Johan
Rocha-Pereira, Joana
author_facet Van Dycke, Jana
Rymenants, Jasper
Neyts, Johan
Rocha-Pereira, Joana
author_sort Van Dycke, Jana
collection PubMed
description Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norovirus-targeting small molecules. We have previously established antiviral assays using this system, identified novel inhibitors and performed additional studies in order to have a first insight into their mechanism of action. As potent and safe anti-norovirus small molecules are urgently needed, we here describe the detailed protocol for a set of assays that will allow the identification of novel norovirus inhibitors.
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spelling pubmed-82562512021-07-16 Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds Van Dycke, Jana Rymenants, Jasper Neyts, Johan Rocha-Pereira, Joana Antivir Chem Chemother Protocol Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norovirus-targeting small molecules. We have previously established antiviral assays using this system, identified novel inhibitors and performed additional studies in order to have a first insight into their mechanism of action. As potent and safe anti-norovirus small molecules are urgently needed, we here describe the detailed protocol for a set of assays that will allow the identification of novel norovirus inhibitors. SAGE Publications 2021-06-29 /pmc/articles/PMC8256251/ /pubmed/34187186 http://dx.doi.org/10.1177/20402066211026852 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Protocol
Van Dycke, Jana
Rymenants, Jasper
Neyts, Johan
Rocha-Pereira, Joana
Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds
title Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds
title_full Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds
title_fullStr Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds
title_full_unstemmed Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds
title_short Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds
title_sort assessment of the anti-norovirus activity in cell culture using the mouse norovirus: identification of active compounds
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256251/
https://www.ncbi.nlm.nih.gov/pubmed/34187186
http://dx.doi.org/10.1177/20402066211026852
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