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The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ
Muscle segment homeobox 2 (MSX2) has been confirmed to be involved in the regulation of early tooth development. However, the role of MSX2 has not been fully elucidated in enamel development. To research the functions of MSX2 in enamel formation, we used a Msx2 (−/−) (KO) mouse model with no full Ms...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256348/ https://www.ncbi.nlm.nih.gov/pubmed/34041852 http://dx.doi.org/10.1111/jcmm.16646 |
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author | Zhang, Juanjuan Xu, Ying Zhao, Ying Bai, Jingkun Xu, Mengge Li, Chuanji Li, Jinyue Ren, Yong Xu, Chang Gao, Yuguang Sun, Yan Liu, Xiaoying |
author_facet | Zhang, Juanjuan Xu, Ying Zhao, Ying Bai, Jingkun Xu, Mengge Li, Chuanji Li, Jinyue Ren, Yong Xu, Chang Gao, Yuguang Sun, Yan Liu, Xiaoying |
author_sort | Zhang, Juanjuan |
collection | PubMed |
description | Muscle segment homeobox 2 (MSX2) has been confirmed to be involved in the regulation of early tooth development. However, the role of MSX2 has not been fully elucidated in enamel development. To research the functions of MSX2 in enamel formation, we used a Msx2 (−/−) (KO) mouse model with no full Msx2 gene. In the present study, the dental appearance and enamel microstructure were detected by scanning electron microscopy and micro‐computed tomography. The results showed that the absence of Msx2 resulted in enamel defects, leading to severe tooth wear in KO mice. To further investigate the mechanism behind the phenotype, we performed detailed histological analyses of the enamel organ in KO mice. We discovered that ameloblasts without Msx2 could secrete a small amount of enamel matrix protein in the early stage. However, the enamel epithelium occurred squamous epithelial hyperplasia and partial keratinization in the enamel organ during subsequent developmental stages. Ameloblasts depolarized and underwent pyroptosis. Overall, during the development of enamel, MSX2 affects the formation of enamel by regulating the function of epithelial cells in the enamel organ. |
format | Online Article Text |
id | pubmed-8256348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82563482021-07-12 The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ Zhang, Juanjuan Xu, Ying Zhao, Ying Bai, Jingkun Xu, Mengge Li, Chuanji Li, Jinyue Ren, Yong Xu, Chang Gao, Yuguang Sun, Yan Liu, Xiaoying J Cell Mol Med Original Articles Muscle segment homeobox 2 (MSX2) has been confirmed to be involved in the regulation of early tooth development. However, the role of MSX2 has not been fully elucidated in enamel development. To research the functions of MSX2 in enamel formation, we used a Msx2 (−/−) (KO) mouse model with no full Msx2 gene. In the present study, the dental appearance and enamel microstructure were detected by scanning electron microscopy and micro‐computed tomography. The results showed that the absence of Msx2 resulted in enamel defects, leading to severe tooth wear in KO mice. To further investigate the mechanism behind the phenotype, we performed detailed histological analyses of the enamel organ in KO mice. We discovered that ameloblasts without Msx2 could secrete a small amount of enamel matrix protein in the early stage. However, the enamel epithelium occurred squamous epithelial hyperplasia and partial keratinization in the enamel organ during subsequent developmental stages. Ameloblasts depolarized and underwent pyroptosis. Overall, during the development of enamel, MSX2 affects the formation of enamel by regulating the function of epithelial cells in the enamel organ. John Wiley and Sons Inc. 2021-05-26 2021-07 /pmc/articles/PMC8256348/ /pubmed/34041852 http://dx.doi.org/10.1111/jcmm.16646 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Zhang, Juanjuan Xu, Ying Zhao, Ying Bai, Jingkun Xu, Mengge Li, Chuanji Li, Jinyue Ren, Yong Xu, Chang Gao, Yuguang Sun, Yan Liu, Xiaoying The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ |
title | The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ |
title_full | The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ |
title_fullStr | The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ |
title_full_unstemmed | The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ |
title_short | The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ |
title_sort | absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256348/ https://www.ncbi.nlm.nih.gov/pubmed/34041852 http://dx.doi.org/10.1111/jcmm.16646 |
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