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A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1
Myogenesis, the process of skeletal muscle formation, is a highly coordinated multistep biological process. Accumulating evidence suggests that long non‐coding RNAs (lncRNAs) are emerging as a gatekeeper in myogenesis. Up to now, most studies on muscle development‐related lncRNAs are mainly focussed...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256363/ https://www.ncbi.nlm.nih.gov/pubmed/33942976 http://dx.doi.org/10.1111/jcmm.16427 |
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author | Chen, Mingming Zhang, Linlin Guo, Yiwen Liu, Xinfeng Song, Yingshen Li, Xin Ding, Xiangbin Guo, Hong |
author_facet | Chen, Mingming Zhang, Linlin Guo, Yiwen Liu, Xinfeng Song, Yingshen Li, Xin Ding, Xiangbin Guo, Hong |
author_sort | Chen, Mingming |
collection | PubMed |
description | Myogenesis, the process of skeletal muscle formation, is a highly coordinated multistep biological process. Accumulating evidence suggests that long non‐coding RNAs (lncRNAs) are emerging as a gatekeeper in myogenesis. Up to now, most studies on muscle development‐related lncRNAs are mainly focussed on humans and mice. In this study, a novel muscle highly expressed lncRNA, named lnc23, localized in nucleus, was found differentially expressed in different stages of embryonic development and myogenic differentiation. The knockdown and over‐expression experiments showed that lnc23 positively regulated the myogenic differentiation of bovine skeletal muscle satellite cells. Then, TMT 10‐plex labelling quantitative proteomics was performed to screen the potentially regulatory proteins of lnc23. Results indicated that lnc23 was involved in the key processes of myogenic differentiation such as cell fusion, further demonstrated that down‐regulation of lnc23 may inhibit myogenic differentiation by reducing signal transduction and cell fusion among cells. Furthermore, RNA pulldown/LC‐MS and RIP experiment illustrated that PFN1 was a binding protein of lnc23. Further, we also found that lnc23 positively regulated the protein expression of RhoA and Rac1, and PFN1 may negatively regulate myogenic differentiation and the expression of its interacting proteins RhoA and Rac1. Hence, we support that lnc23 may reduce the inhibiting effect of PFN1 on RhoA and Rac1 by binding to PFN1, thereby promoting myogenic differentiation. In short, the novel identified lnc23 promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1. |
format | Online Article Text |
id | pubmed-8256363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82563632021-07-12 A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1 Chen, Mingming Zhang, Linlin Guo, Yiwen Liu, Xinfeng Song, Yingshen Li, Xin Ding, Xiangbin Guo, Hong J Cell Mol Med Original Articles Myogenesis, the process of skeletal muscle formation, is a highly coordinated multistep biological process. Accumulating evidence suggests that long non‐coding RNAs (lncRNAs) are emerging as a gatekeeper in myogenesis. Up to now, most studies on muscle development‐related lncRNAs are mainly focussed on humans and mice. In this study, a novel muscle highly expressed lncRNA, named lnc23, localized in nucleus, was found differentially expressed in different stages of embryonic development and myogenic differentiation. The knockdown and over‐expression experiments showed that lnc23 positively regulated the myogenic differentiation of bovine skeletal muscle satellite cells. Then, TMT 10‐plex labelling quantitative proteomics was performed to screen the potentially regulatory proteins of lnc23. Results indicated that lnc23 was involved in the key processes of myogenic differentiation such as cell fusion, further demonstrated that down‐regulation of lnc23 may inhibit myogenic differentiation by reducing signal transduction and cell fusion among cells. Furthermore, RNA pulldown/LC‐MS and RIP experiment illustrated that PFN1 was a binding protein of lnc23. Further, we also found that lnc23 positively regulated the protein expression of RhoA and Rac1, and PFN1 may negatively regulate myogenic differentiation and the expression of its interacting proteins RhoA and Rac1. Hence, we support that lnc23 may reduce the inhibiting effect of PFN1 on RhoA and Rac1 by binding to PFN1, thereby promoting myogenic differentiation. In short, the novel identified lnc23 promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1. John Wiley and Sons Inc. 2021-05-04 2021-07 /pmc/articles/PMC8256363/ /pubmed/33942976 http://dx.doi.org/10.1111/jcmm.16427 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Chen, Mingming Zhang, Linlin Guo, Yiwen Liu, Xinfeng Song, Yingshen Li, Xin Ding, Xiangbin Guo, Hong A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1 |
title | A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1 |
title_full | A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1 |
title_fullStr | A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1 |
title_full_unstemmed | A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1 |
title_short | A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1‐RhoA/Rac1 |
title_sort | novel lncrna promotes myogenesis of bovine skeletal muscle satellite cells via pfn1‐rhoa/rac1 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256363/ https://www.ncbi.nlm.nih.gov/pubmed/33942976 http://dx.doi.org/10.1111/jcmm.16427 |
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