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Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents
INTRODUCTION: Recombinant bovine interferon alpha (rBoIFN-α) has been demonstrated to have antiviral activity. However, no conduct of acute or chronic toxicity tests has been reported. MATERIAL AND METHODS: Specific pathogen-free Sprague Dawley rats were administered doses at different concentration...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sciendo
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256469/ https://www.ncbi.nlm.nih.gov/pubmed/34250303 http://dx.doi.org/10.2478/jvetres-2021-0023 |
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author | Yu, Hai-Yang Gao, Dong-Mei Zhou, Wei Xia, Bing-Bing He, Zhi-Yuan Wu, Bo Jiang, Min-Zhi Wang, Ming-Li Zhao, Jun |
author_facet | Yu, Hai-Yang Gao, Dong-Mei Zhou, Wei Xia, Bing-Bing He, Zhi-Yuan Wu, Bo Jiang, Min-Zhi Wang, Ming-Li Zhao, Jun |
author_sort | Yu, Hai-Yang |
collection | PubMed |
description | INTRODUCTION: Recombinant bovine interferon alpha (rBoIFN-α) has been demonstrated to have antiviral activity. However, no conduct of acute or chronic toxicity tests has been reported. MATERIAL AND METHODS: Specific pathogen-free Sprague Dawley rats were administered doses at different concentrations through intraperitoneal or intravenous injection. After the administration (single for an acute toxicity test over 14 days or daily for a sub-chronic toxicity test over 30 days), the rats’ behaviour and other indicators and the degree of toxic reaction were continuously monitored. Blood was collected for haematological and serum biochemical examinations. At the end of the experiments, the rats were sacrificed for necropsy and histopathological tissue analysis. RESULTS: The external performance, behaviour characteristics, and changes in body temperature and body weight of the rats in each subgroup were comparable to the normal control subgroup. Except for a few cases, there were no lesions in the viscera’s pathological structures, and the blood parameters and biochemical indicators were not noticeably different from those of the control subgroup. CONCLUSION: This study suggests that rBoIFN-α seems to be safe for rats, and its use may foster the development of the cattle industry in China by protecting livestock health. |
format | Online Article Text |
id | pubmed-8256469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-82564692021-07-09 Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents Yu, Hai-Yang Gao, Dong-Mei Zhou, Wei Xia, Bing-Bing He, Zhi-Yuan Wu, Bo Jiang, Min-Zhi Wang, Ming-Li Zhao, Jun J Vet Res Research Article INTRODUCTION: Recombinant bovine interferon alpha (rBoIFN-α) has been demonstrated to have antiviral activity. However, no conduct of acute or chronic toxicity tests has been reported. MATERIAL AND METHODS: Specific pathogen-free Sprague Dawley rats were administered doses at different concentrations through intraperitoneal or intravenous injection. After the administration (single for an acute toxicity test over 14 days or daily for a sub-chronic toxicity test over 30 days), the rats’ behaviour and other indicators and the degree of toxic reaction were continuously monitored. Blood was collected for haematological and serum biochemical examinations. At the end of the experiments, the rats were sacrificed for necropsy and histopathological tissue analysis. RESULTS: The external performance, behaviour characteristics, and changes in body temperature and body weight of the rats in each subgroup were comparable to the normal control subgroup. Except for a few cases, there were no lesions in the viscera’s pathological structures, and the blood parameters and biochemical indicators were not noticeably different from those of the control subgroup. CONCLUSION: This study suggests that rBoIFN-α seems to be safe for rats, and its use may foster the development of the cattle industry in China by protecting livestock health. Sciendo 2021-05-16 /pmc/articles/PMC8256469/ /pubmed/34250303 http://dx.doi.org/10.2478/jvetres-2021-0023 Text en © 2021 H.Y. Yu et al. published by Sciendo https://creativecommons.org/licenses/by-nc-nd/3.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. |
spellingShingle | Research Article Yu, Hai-Yang Gao, Dong-Mei Zhou, Wei Xia, Bing-Bing He, Zhi-Yuan Wu, Bo Jiang, Min-Zhi Wang, Ming-Li Zhao, Jun Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents |
title | Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents |
title_full | Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents |
title_fullStr | Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents |
title_full_unstemmed | Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents |
title_short | Acute and Sub-chronic Toxicity Study of Recombinant Bovine Interferon Alpha in Rodents |
title_sort | acute and sub-chronic toxicity study of recombinant bovine interferon alpha in rodents |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256469/ https://www.ncbi.nlm.nih.gov/pubmed/34250303 http://dx.doi.org/10.2478/jvetres-2021-0023 |
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