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Recombinant SARS-CoV-2 envelope protein traffics to the trans-Golgi network following amphipol-mediated delivery into human cells

The severe acute respiratory syndrome coronavirus 2 envelope protein (S2-E) is a conserved membrane protein that is important for coronavirus (CoV) assembly and budding. Here, we describe the recombinant expression and purification of S2-E in amphipol-class amphipathic polymer solutions, which solub...

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Detalles Bibliográficos
Autores principales: Hutchison, James M., Capone, Ricardo, Luu, Dustin D., Shah, Karan H., Hadziselimovic, Arina, Van Horn, Wade D., Sanders, Charles R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256659/
https://www.ncbi.nlm.nih.gov/pubmed/34237302
http://dx.doi.org/10.1016/j.jbc.2021.100940