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Recombinant SARS-CoV-2 envelope protein traffics to the trans-Golgi network following amphipol-mediated delivery into human cells
The severe acute respiratory syndrome coronavirus 2 envelope protein (S2-E) is a conserved membrane protein that is important for coronavirus (CoV) assembly and budding. Here, we describe the recombinant expression and purification of S2-E in amphipol-class amphipathic polymer solutions, which solub...
Autores principales: | Hutchison, James M., Capone, Ricardo, Luu, Dustin D., Shah, Karan H., Hadziselimovic, Arina, Van Horn, Wade D., Sanders, Charles R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256659/ https://www.ncbi.nlm.nih.gov/pubmed/34237302 http://dx.doi.org/10.1016/j.jbc.2021.100940 |
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