Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA

Bisulfite sequencing detects 5mC and 5hmC at single-base resolution. However, bisulfite treatment damages DNA, which results in fragmentation, DNA loss, and biased sequencing data. To overcome these problems, enzymatic methyl-seq (EM-seq) was developed. This method detects 5mC and 5hmC using two set...

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Detalles Bibliográficos
Autores principales: Vaisvila, Romualdas, Ponnaluri, V.K. Chaithanya, Sun, Zhiyi, Langhorst, Bradley W., Saleh, Lana, Guan, Shengxi, Dai, Nan, Campbell, Matthew A., Sexton, Brittany S., Marks, Katherine, Samaranayake, Mala, Samuelson, James C., Church, Heidi E., Tamanaha, Esta, Corrêa, Ivan R., Pradhan, Sriharsa, Dimalanta, Eileen T., Evans, Thomas C., Williams, Louise, Davis, Theodore B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256858/
https://www.ncbi.nlm.nih.gov/pubmed/34140313
http://dx.doi.org/10.1101/gr.266551.120

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