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Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates
Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among differe...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256890/ https://www.ncbi.nlm.nih.gov/pubmed/34235095 http://dx.doi.org/10.3389/fcimb.2021.685068 |
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author | Qian, Changrui Liu, Hongmao Cao, Jiawei Ji, Yongan Lu, Wei Lu, Junwan Li, Aifang Zhu, Xinyi Shen, Kai Xu, Haili Chen, Qianqian Zhou, Wangxiao Lu, Hongyun Lin, Hailong Zhang, Xueya Li, Qiaoling Lin, Xi Li, Kewei Xu, Teng Zhu, Mei Bao, Qiyu Zhang, Hailin |
author_facet | Qian, Changrui Liu, Hongmao Cao, Jiawei Ji, Yongan Lu, Wei Lu, Junwan Li, Aifang Zhu, Xinyi Shen, Kai Xu, Haili Chen, Qianqian Zhou, Wangxiao Lu, Hongyun Lin, Hailong Zhang, Xueya Li, Qiaoling Lin, Xi Li, Kewei Xu, Teng Zhu, Mei Bao, Qiyu Zhang, Hailin |
author_sort | Qian, Changrui |
collection | PubMed |
description | Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of floR in 430 Pseudomonas aeruginosa isolates from human clinical samples and identified three types of floR genes (designated floR, floR-T1 and floR-T2) in these isolates, with floR-T1 the most prevalent (5.3%, 23/430). FloR-T2 was a novel floR variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, floR-T1 and floR-T2 identified in P. aeruginosa strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn4371-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two floR variants could be induced by florfenicol or chloramphenicol. These results indicated that the two floR variants played an essential role in the host’s resistance to amphenicol and the spreading of these floR variants might be related with the Tn4371 family ICE. |
format | Online Article Text |
id | pubmed-8256890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82568902021-07-06 Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates Qian, Changrui Liu, Hongmao Cao, Jiawei Ji, Yongan Lu, Wei Lu, Junwan Li, Aifang Zhu, Xinyi Shen, Kai Xu, Haili Chen, Qianqian Zhou, Wangxiao Lu, Hongyun Lin, Hailong Zhang, Xueya Li, Qiaoling Lin, Xi Li, Kewei Xu, Teng Zhu, Mei Bao, Qiyu Zhang, Hailin Front Cell Infect Microbiol Cellular and Infection Microbiology Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of floR in 430 Pseudomonas aeruginosa isolates from human clinical samples and identified three types of floR genes (designated floR, floR-T1 and floR-T2) in these isolates, with floR-T1 the most prevalent (5.3%, 23/430). FloR-T2 was a novel floR variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, floR-T1 and floR-T2 identified in P. aeruginosa strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn4371-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two floR variants could be induced by florfenicol or chloramphenicol. These results indicated that the two floR variants played an essential role in the host’s resistance to amphenicol and the spreading of these floR variants might be related with the Tn4371 family ICE. Frontiers Media S.A. 2021-06-21 /pmc/articles/PMC8256890/ /pubmed/34235095 http://dx.doi.org/10.3389/fcimb.2021.685068 Text en Copyright © 2021 Qian, Liu, Cao, Ji, Lu, Lu, Li, Zhu, Shen, Xu, Chen, Zhou, Lu, Lin, Zhang, Li, Lin, Li, Xu, Zhu, Bao and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Qian, Changrui Liu, Hongmao Cao, Jiawei Ji, Yongan Lu, Wei Lu, Junwan Li, Aifang Zhu, Xinyi Shen, Kai Xu, Haili Chen, Qianqian Zhou, Wangxiao Lu, Hongyun Lin, Hailong Zhang, Xueya Li, Qiaoling Lin, Xi Li, Kewei Xu, Teng Zhu, Mei Bao, Qiyu Zhang, Hailin Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
title | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
title_full | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
title_fullStr | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
title_full_unstemmed | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
title_short | Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates |
title_sort | identification of flor variants associated with a novel tn4371-like integrative and conjugative element in clinical pseudomonas aeruginosa isolates |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256890/ https://www.ncbi.nlm.nih.gov/pubmed/34235095 http://dx.doi.org/10.3389/fcimb.2021.685068 |
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