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Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates

Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among differe...

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Autores principales: Qian, Changrui, Liu, Hongmao, Cao, Jiawei, Ji, Yongan, Lu, Wei, Lu, Junwan, Li, Aifang, Zhu, Xinyi, Shen, Kai, Xu, Haili, Chen, Qianqian, Zhou, Wangxiao, Lu, Hongyun, Lin, Hailong, Zhang, Xueya, Li, Qiaoling, Lin, Xi, Li, Kewei, Xu, Teng, Zhu, Mei, Bao, Qiyu, Zhang, Hailin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256890/
https://www.ncbi.nlm.nih.gov/pubmed/34235095
http://dx.doi.org/10.3389/fcimb.2021.685068
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author Qian, Changrui
Liu, Hongmao
Cao, Jiawei
Ji, Yongan
Lu, Wei
Lu, Junwan
Li, Aifang
Zhu, Xinyi
Shen, Kai
Xu, Haili
Chen, Qianqian
Zhou, Wangxiao
Lu, Hongyun
Lin, Hailong
Zhang, Xueya
Li, Qiaoling
Lin, Xi
Li, Kewei
Xu, Teng
Zhu, Mei
Bao, Qiyu
Zhang, Hailin
author_facet Qian, Changrui
Liu, Hongmao
Cao, Jiawei
Ji, Yongan
Lu, Wei
Lu, Junwan
Li, Aifang
Zhu, Xinyi
Shen, Kai
Xu, Haili
Chen, Qianqian
Zhou, Wangxiao
Lu, Hongyun
Lin, Hailong
Zhang, Xueya
Li, Qiaoling
Lin, Xi
Li, Kewei
Xu, Teng
Zhu, Mei
Bao, Qiyu
Zhang, Hailin
author_sort Qian, Changrui
collection PubMed
description Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of floR in 430 Pseudomonas aeruginosa isolates from human clinical samples and identified three types of floR genes (designated floR, floR-T1 and floR-T2) in these isolates, with floR-T1 the most prevalent (5.3%, 23/430). FloR-T2 was a novel floR variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, floR-T1 and floR-T2 identified in P. aeruginosa strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn4371-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two floR variants could be induced by florfenicol or chloramphenicol. These results indicated that the two floR variants played an essential role in the host’s resistance to amphenicol and the spreading of these floR variants might be related with the Tn4371 family ICE.
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spelling pubmed-82568902021-07-06 Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates Qian, Changrui Liu, Hongmao Cao, Jiawei Ji, Yongan Lu, Wei Lu, Junwan Li, Aifang Zhu, Xinyi Shen, Kai Xu, Haili Chen, Qianqian Zhou, Wangxiao Lu, Hongyun Lin, Hailong Zhang, Xueya Li, Qiaoling Lin, Xi Li, Kewei Xu, Teng Zhu, Mei Bao, Qiyu Zhang, Hailin Front Cell Infect Microbiol Cellular and Infection Microbiology Florfenicol is widely used to control respiratory diseases and intestinal infections in food animals. However, there are increasing reports about florfenicol resistance of various clinical pathogens. floR is a key resistance gene that mediates resistance to florfenicol and could spread among different bacteria. Here, we investigated the prevalence of floR in 430 Pseudomonas aeruginosa isolates from human clinical samples and identified three types of floR genes (designated floR, floR-T1 and floR-T2) in these isolates, with floR-T1 the most prevalent (5.3%, 23/430). FloR-T2 was a novel floR variant identified in this study, and exhibited less identity with other FloR proteins than FloRv. Moreover, floR-T1 and floR-T2 identified in P. aeruginosa strain TL1285 were functionally active and located on multi-drug resistance region of a novel incomplete Tn4371-like integrative and conjugative elements (ICE) in the chromosome. The expression of the two floR variants could be induced by florfenicol or chloramphenicol. These results indicated that the two floR variants played an essential role in the host’s resistance to amphenicol and the spreading of these floR variants might be related with the Tn4371 family ICE. Frontiers Media S.A. 2021-06-21 /pmc/articles/PMC8256890/ /pubmed/34235095 http://dx.doi.org/10.3389/fcimb.2021.685068 Text en Copyright © 2021 Qian, Liu, Cao, Ji, Lu, Lu, Li, Zhu, Shen, Xu, Chen, Zhou, Lu, Lin, Zhang, Li, Lin, Li, Xu, Zhu, Bao and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Qian, Changrui
Liu, Hongmao
Cao, Jiawei
Ji, Yongan
Lu, Wei
Lu, Junwan
Li, Aifang
Zhu, Xinyi
Shen, Kai
Xu, Haili
Chen, Qianqian
Zhou, Wangxiao
Lu, Hongyun
Lin, Hailong
Zhang, Xueya
Li, Qiaoling
Lin, Xi
Li, Kewei
Xu, Teng
Zhu, Mei
Bao, Qiyu
Zhang, Hailin
Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates
title Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates
title_full Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates
title_fullStr Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates
title_full_unstemmed Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates
title_short Identification of floR Variants Associated With a Novel Tn4371-Like Integrative and Conjugative Element in Clinical Pseudomonas aeruginosa Isolates
title_sort identification of flor variants associated with a novel tn4371-like integrative and conjugative element in clinical pseudomonas aeruginosa isolates
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256890/
https://www.ncbi.nlm.nih.gov/pubmed/34235095
http://dx.doi.org/10.3389/fcimb.2021.685068
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