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Impact of Dietary Protein Content on Soil Bacterial and Fungal Communities in a Rice–Crab Co-culture System

Although co-culture of paddy fields with aquatic animals is lucrative, the ecological impacts of high-protein content entering the agricultural soil via animal pellet feed and feces have not been well studied. Moreover, the effects of dietary protein on soils and soil microbes remain unclear. To elu...

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Autores principales: Li, Yingdong, Li, Lisong, Yu, Yilin, Hu, Qingbiao, Li, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256891/
https://www.ncbi.nlm.nih.gov/pubmed/34234767
http://dx.doi.org/10.3389/fmicb.2021.696427
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author Li, Yingdong
Li, Lisong
Yu, Yilin
Hu, Qingbiao
Li, Xiaodong
author_facet Li, Yingdong
Li, Lisong
Yu, Yilin
Hu, Qingbiao
Li, Xiaodong
author_sort Li, Yingdong
collection PubMed
description Although co-culture of paddy fields with aquatic animals is lucrative, the ecological impacts of high-protein content entering the agricultural soil via animal pellet feed and feces have not been well studied. Moreover, the effects of dietary protein on soils and soil microbes remain unclear. To elucidate this, we examined soil bacterial and fungal community composition and temporal changes in paddy fields subjected to different protein-content diets via 16S/18S rRNA gene amplicon sequencing analysis with a high-throughput next-generation sequencer. MiSeq sequencing revealed that protein content significantly impacted fungal community structure. High-protein diets reduced bacterial community diversity and relative abundance in both July and October. The phylum-level bacterial taxonomic composition was not affected by diet treatment, while in fungi, a major phylum-level shift was evident. Hierarchically clustered analysis showed that high-protein diets significantly reduced the relative abundance of Brevundimonas in both July and October. Saprotrophic macrofungal diversity was negatively related to dietary protein content. Considering microbial community structure and environmental factors, ca. 15% protein content is appropriate for the rice-crab co-culture system that we studied.
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spelling pubmed-82568912021-07-06 Impact of Dietary Protein Content on Soil Bacterial and Fungal Communities in a Rice–Crab Co-culture System Li, Yingdong Li, Lisong Yu, Yilin Hu, Qingbiao Li, Xiaodong Front Microbiol Microbiology Although co-culture of paddy fields with aquatic animals is lucrative, the ecological impacts of high-protein content entering the agricultural soil via animal pellet feed and feces have not been well studied. Moreover, the effects of dietary protein on soils and soil microbes remain unclear. To elucidate this, we examined soil bacterial and fungal community composition and temporal changes in paddy fields subjected to different protein-content diets via 16S/18S rRNA gene amplicon sequencing analysis with a high-throughput next-generation sequencer. MiSeq sequencing revealed that protein content significantly impacted fungal community structure. High-protein diets reduced bacterial community diversity and relative abundance in both July and October. The phylum-level bacterial taxonomic composition was not affected by diet treatment, while in fungi, a major phylum-level shift was evident. Hierarchically clustered analysis showed that high-protein diets significantly reduced the relative abundance of Brevundimonas in both July and October. Saprotrophic macrofungal diversity was negatively related to dietary protein content. Considering microbial community structure and environmental factors, ca. 15% protein content is appropriate for the rice-crab co-culture system that we studied. Frontiers Media S.A. 2021-06-21 /pmc/articles/PMC8256891/ /pubmed/34234767 http://dx.doi.org/10.3389/fmicb.2021.696427 Text en Copyright © 2021 Li, Li, Yu, Hu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Yingdong
Li, Lisong
Yu, Yilin
Hu, Qingbiao
Li, Xiaodong
Impact of Dietary Protein Content on Soil Bacterial and Fungal Communities in a Rice–Crab Co-culture System
title Impact of Dietary Protein Content on Soil Bacterial and Fungal Communities in a Rice–Crab Co-culture System
title_full Impact of Dietary Protein Content on Soil Bacterial and Fungal Communities in a Rice–Crab Co-culture System
title_fullStr Impact of Dietary Protein Content on Soil Bacterial and Fungal Communities in a Rice–Crab Co-culture System
title_full_unstemmed Impact of Dietary Protein Content on Soil Bacterial and Fungal Communities in a Rice–Crab Co-culture System
title_short Impact of Dietary Protein Content on Soil Bacterial and Fungal Communities in a Rice–Crab Co-culture System
title_sort impact of dietary protein content on soil bacterial and fungal communities in a rice–crab co-culture system
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256891/
https://www.ncbi.nlm.nih.gov/pubmed/34234767
http://dx.doi.org/10.3389/fmicb.2021.696427
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