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Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice

The behavioural responses to nicotine involve appetite-regulatory hormones; however, the effects of the anorexigenic hormone amylin on reward-related behaviours induced by nicotine remain to be established. Previous studies have shown that the amylinergic pathway regulates behavioural responses to a...

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Autores principales: Aranäs, Cajsa, Vestlund, Jesper, Witley, Sarah, Edvardsson, Christian E., Kalafateli, Aimilia Lydia, Jerlhag, Elisabet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257032/
https://www.ncbi.nlm.nih.gov/pubmed/34234676
http://dx.doi.org/10.3389/fphar.2021.685631
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author Aranäs, Cajsa
Vestlund, Jesper
Witley, Sarah
Edvardsson, Christian E.
Kalafateli, Aimilia Lydia
Jerlhag, Elisabet
author_facet Aranäs, Cajsa
Vestlund, Jesper
Witley, Sarah
Edvardsson, Christian E.
Kalafateli, Aimilia Lydia
Jerlhag, Elisabet
author_sort Aranäs, Cajsa
collection PubMed
description The behavioural responses to nicotine involve appetite-regulatory hormones; however, the effects of the anorexigenic hormone amylin on reward-related behaviours induced by nicotine remain to be established. Previous studies have shown that the amylinergic pathway regulates behavioural responses to alcohol, amphetamine and cocaine. Here, we evaluated the effects of salmon calcitonin (sCT), an amylin and calcitonin receptor (CTR) agonist, on nicotine-induced locomotor stimulation and sensitisation as well as dopamine release in the nucleus accumbens (NAc) shell. Moreover, we investigated the effects of sCT on the acquisition and expression of nicotine-induced reward in the conditioned place preference (CPP) paradigm. Finally, we performed Western Blot experiments in an attempt to identify the levels of the amylin receptor components CTRa, CTRb, and RAMP1 in reward-related areas of mice responding differently to repeated injections of sCT and nicotine in the locomotor sensitisation test. We found that sCT blocked nicotine’s stimulatory and dopamine-releasing effects and prevented its ability to cause locomotor sensitisation. On the other hand, sCT did not alter nicotine-induced acquisition and expression of CPP. Lastly, sCT-nicotine treated mice from the locomotor sensitisation experiment displayed higher levels of total CTR, i.e. CTRa and CTRb together, in the reward-processing laterodorsal tegmental area (LDTg) of the brain compared to mice treated with vehicle-nicotine. Overall, the present data reveal that activation of CTR or/and amylin receptors attenuates certain nicotine-induced behaviours in male mice, further contributing to the understanding of appetite-regulatory peptides in reward regulation.
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spelling pubmed-82570322021-07-06 Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice Aranäs, Cajsa Vestlund, Jesper Witley, Sarah Edvardsson, Christian E. Kalafateli, Aimilia Lydia Jerlhag, Elisabet Front Pharmacol Pharmacology The behavioural responses to nicotine involve appetite-regulatory hormones; however, the effects of the anorexigenic hormone amylin on reward-related behaviours induced by nicotine remain to be established. Previous studies have shown that the amylinergic pathway regulates behavioural responses to alcohol, amphetamine and cocaine. Here, we evaluated the effects of salmon calcitonin (sCT), an amylin and calcitonin receptor (CTR) agonist, on nicotine-induced locomotor stimulation and sensitisation as well as dopamine release in the nucleus accumbens (NAc) shell. Moreover, we investigated the effects of sCT on the acquisition and expression of nicotine-induced reward in the conditioned place preference (CPP) paradigm. Finally, we performed Western Blot experiments in an attempt to identify the levels of the amylin receptor components CTRa, CTRb, and RAMP1 in reward-related areas of mice responding differently to repeated injections of sCT and nicotine in the locomotor sensitisation test. We found that sCT blocked nicotine’s stimulatory and dopamine-releasing effects and prevented its ability to cause locomotor sensitisation. On the other hand, sCT did not alter nicotine-induced acquisition and expression of CPP. Lastly, sCT-nicotine treated mice from the locomotor sensitisation experiment displayed higher levels of total CTR, i.e. CTRa and CTRb together, in the reward-processing laterodorsal tegmental area (LDTg) of the brain compared to mice treated with vehicle-nicotine. Overall, the present data reveal that activation of CTR or/and amylin receptors attenuates certain nicotine-induced behaviours in male mice, further contributing to the understanding of appetite-regulatory peptides in reward regulation. Frontiers Media S.A. 2021-06-21 /pmc/articles/PMC8257032/ /pubmed/34234676 http://dx.doi.org/10.3389/fphar.2021.685631 Text en Copyright © 2021 Aranäs, Vestlund, Witley, Edvardsson, Kalafateli and Jerlhag. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Aranäs, Cajsa
Vestlund, Jesper
Witley, Sarah
Edvardsson, Christian E.
Kalafateli, Aimilia Lydia
Jerlhag, Elisabet
Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice
title Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice
title_full Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice
title_fullStr Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice
title_full_unstemmed Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice
title_short Salmon Calcitonin Attenuates Some Behavioural Responses to Nicotine in Male Mice
title_sort salmon calcitonin attenuates some behavioural responses to nicotine in male mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257032/
https://www.ncbi.nlm.nih.gov/pubmed/34234676
http://dx.doi.org/10.3389/fphar.2021.685631
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