Cargando…

Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding

Time-restricted feeding (TRF) mode is a potential strategy in improving the health and production of farm animals. However, the effect of TRF on microbiota and their metabolism in the large intestine of the host remains unclear. Therefore, the present study aimed to investigate the responses of micr...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Hongyu, Xia, Pengke, Lu, Zhiyang, Su, Yong, Zhu, Weiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258120/
https://www.ncbi.nlm.nih.gov/pubmed/34239912
http://dx.doi.org/10.3389/fvets.2021.681202
_version_ 1783718441670148096
author Wang, Hongyu
Xia, Pengke
Lu, Zhiyang
Su, Yong
Zhu, Weiyun
author_facet Wang, Hongyu
Xia, Pengke
Lu, Zhiyang
Su, Yong
Zhu, Weiyun
author_sort Wang, Hongyu
collection PubMed
description Time-restricted feeding (TRF) mode is a potential strategy in improving the health and production of farm animals. However, the effect of TRF on microbiota and their metabolism in the large intestine of the host remains unclear. Therefore, the present study aimed to investigate the responses of microbiome and metabolome induced by TRF based on a growing-pig model. Twelve crossbred growing barrows were randomly allotted into two groups with six replicates (1 pig/pen), namely, the free-access feeding group (FA) and TRF group. Pigs in the FA group were fed free access while the TRF group were fed free access within a regular time three times per day at 07:00–08:00, 12:00–13:00, and 18:00–19:00, respectively. Results showed that the concentrations of NH4-N, putrescine, cadaverine, spermidine, spermine, total biogenic amines, isobutyrate, butyrate, isovalerate, total SCFA, and lactate were increased while the pH value in the colonic digesta and the concentration of acetate was decreased in the TRF group. The Shannon index was significantly increased in the TRF group; however, no significant effects were found in the Fisher index, Simpson index, ACE index, Chao1 index, and observed species between the two groups. In the TRF group, the relative abundances of Prevotella 1 and Eubacterium ruminantium group were significantly increased while the relative abundances of Clostridium sensu sticto 1, Lactobacillus, and Eubacterium coprostanoligenes group were decreased compared with the FA group. PLS-DA analysis revealed an obvious and regular variation between the FA and TRF groups, further pathway enrichment analysis showed that these differential features were mainly enriched in pyrimidine metabolism, nicotinate and nicotinamide metabolism, glycerolipid metabolism, and fructose and mannose metabolism. In addition, Pearson's correlation analysis indicated that the changes in the microbial genera were correlated with the colonic metabolites. In conclusion, these results together indicated that although the overall microbial composition in the colon was not changed, TRF induced the gradient changes of the nutrients and metabolites which were correlated with certain microbial genera including Lactobacillus, Eubacterium_ruminantium group, Eubacterium coprostanoligenes group, Prevotella 1, and Clostridium sensu sticto 1. However, more studies are needed to understand the impacts of TRF on the health and metabolism of growing pigs.
format Online
Article
Text
id pubmed-8258120
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82581202021-07-07 Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding Wang, Hongyu Xia, Pengke Lu, Zhiyang Su, Yong Zhu, Weiyun Front Vet Sci Veterinary Science Time-restricted feeding (TRF) mode is a potential strategy in improving the health and production of farm animals. However, the effect of TRF on microbiota and their metabolism in the large intestine of the host remains unclear. Therefore, the present study aimed to investigate the responses of microbiome and metabolome induced by TRF based on a growing-pig model. Twelve crossbred growing barrows were randomly allotted into two groups with six replicates (1 pig/pen), namely, the free-access feeding group (FA) and TRF group. Pigs in the FA group were fed free access while the TRF group were fed free access within a regular time three times per day at 07:00–08:00, 12:00–13:00, and 18:00–19:00, respectively. Results showed that the concentrations of NH4-N, putrescine, cadaverine, spermidine, spermine, total biogenic amines, isobutyrate, butyrate, isovalerate, total SCFA, and lactate were increased while the pH value in the colonic digesta and the concentration of acetate was decreased in the TRF group. The Shannon index was significantly increased in the TRF group; however, no significant effects were found in the Fisher index, Simpson index, ACE index, Chao1 index, and observed species between the two groups. In the TRF group, the relative abundances of Prevotella 1 and Eubacterium ruminantium group were significantly increased while the relative abundances of Clostridium sensu sticto 1, Lactobacillus, and Eubacterium coprostanoligenes group were decreased compared with the FA group. PLS-DA analysis revealed an obvious and regular variation between the FA and TRF groups, further pathway enrichment analysis showed that these differential features were mainly enriched in pyrimidine metabolism, nicotinate and nicotinamide metabolism, glycerolipid metabolism, and fructose and mannose metabolism. In addition, Pearson's correlation analysis indicated that the changes in the microbial genera were correlated with the colonic metabolites. In conclusion, these results together indicated that although the overall microbial composition in the colon was not changed, TRF induced the gradient changes of the nutrients and metabolites which were correlated with certain microbial genera including Lactobacillus, Eubacterium_ruminantium group, Eubacterium coprostanoligenes group, Prevotella 1, and Clostridium sensu sticto 1. However, more studies are needed to understand the impacts of TRF on the health and metabolism of growing pigs. Frontiers Media S.A. 2021-06-22 /pmc/articles/PMC8258120/ /pubmed/34239912 http://dx.doi.org/10.3389/fvets.2021.681202 Text en Copyright © 2021 Wang, Xia, Lu, Su and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Wang, Hongyu
Xia, Pengke
Lu, Zhiyang
Su, Yong
Zhu, Weiyun
Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding
title Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding
title_full Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding
title_fullStr Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding
title_full_unstemmed Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding
title_short Metabolome-Microbiome Responses of Growing Pigs Induced by Time-Restricted Feeding
title_sort metabolome-microbiome responses of growing pigs induced by time-restricted feeding
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258120/
https://www.ncbi.nlm.nih.gov/pubmed/34239912
http://dx.doi.org/10.3389/fvets.2021.681202
work_keys_str_mv AT wanghongyu metabolomemicrobiomeresponsesofgrowingpigsinducedbytimerestrictedfeeding
AT xiapengke metabolomemicrobiomeresponsesofgrowingpigsinducedbytimerestrictedfeeding
AT luzhiyang metabolomemicrobiomeresponsesofgrowingpigsinducedbytimerestrictedfeeding
AT suyong metabolomemicrobiomeresponsesofgrowingpigsinducedbytimerestrictedfeeding
AT zhuweiyun metabolomemicrobiomeresponsesofgrowingpigsinducedbytimerestrictedfeeding