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Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus

Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato...

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Autores principales: Yue, Hao, Huang, Li-Ping, Lu, Ding-Yi-Hui, Zhang, Zhan-Hong, Zhang, Zhuo, Zhang, De-Yong, Zheng, Li-Min, Gao, Yang, Tan, Xin-Qiu, Zhou, Xu-Guo, Shi, Xiao-Bin, Liu, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258350/
https://www.ncbi.nlm.nih.gov/pubmed/34239512
http://dx.doi.org/10.3389/fmicb.2021.693574
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author Yue, Hao
Huang, Li-Ping
Lu, Ding-Yi-Hui
Zhang, Zhan-Hong
Zhang, Zhuo
Zhang, De-Yong
Zheng, Li-Min
Gao, Yang
Tan, Xin-Qiu
Zhou, Xu-Guo
Shi, Xiao-Bin
Liu, Yong
author_facet Yue, Hao
Huang, Li-Ping
Lu, Ding-Yi-Hui
Zhang, Zhan-Hong
Zhang, Zhuo
Zhang, De-Yong
Zheng, Li-Min
Gao, Yang
Tan, Xin-Qiu
Zhou, Xu-Guo
Shi, Xiao-Bin
Liu, Yong
author_sort Yue, Hao
collection PubMed
description Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of B. tabaci and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and B. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants.
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spelling pubmed-82583502021-07-07 Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus Yue, Hao Huang, Li-Ping Lu, Ding-Yi-Hui Zhang, Zhan-Hong Zhang, Zhuo Zhang, De-Yong Zheng, Li-Min Gao, Yang Tan, Xin-Qiu Zhou, Xu-Guo Shi, Xiao-Bin Liu, Yong Front Microbiol Microbiology Tomato chlorosis virus (ToCV), is one of the most devastating cultivated tomato viruses, seriously threatened the growth of crops worldwide. As the vector of ToCV, the whitefly Bemisia tabaci Mediterranean (MED) is mainly responsible for the rapid spread of ToCV. The current understanding of tomato plant responses to this virus and B. tabaci is very limited. To understand the molecular mechanism of the interaction between tomato, ToCV and B. tabaci, we adopted a next-generation sequencing approach to decipher miRNAs and mRNAs that are differentially expressed under the infection of B. tabaci and ToCV in tomato plants. Our data revealed that 6199 mRNAs were significantly regulated, and the differentially expressed genes were most significantly associated with the plant-pathogen interaction, the MAPK signaling pathway, the glyoxylate, and the carbon fixation in photosynthetic organisms and photosynthesis related proteins. Concomitantly, 242 differentially expressed miRNAs were detected, including novel putative miRNAs. Sly-miR159, sly-miR9471b-3p, and sly-miR162 were the most expressed miRNAs in each sample compare to control group. Moreover, we compared the similarities and differences of gene expression in tomato plant caused by infection or co-infection of B. tabaci and ToCV. Taken together, the analysis reported in this article lays a solid foundation for further research on the interaction between tomato, ToCV and B. tabaci, and provide evidence for the identification of potential key genes that influences virus transmission in tomato plants. Frontiers Media S.A. 2021-06-22 /pmc/articles/PMC8258350/ /pubmed/34239512 http://dx.doi.org/10.3389/fmicb.2021.693574 Text en Copyright © 2021 Yue, Huang, Lu, Zhang, Zhang, Zhang, Zheng, Gao, Tan, Zhou, Shi and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yue, Hao
Huang, Li-Ping
Lu, Ding-Yi-Hui
Zhang, Zhan-Hong
Zhang, Zhuo
Zhang, De-Yong
Zheng, Li-Min
Gao, Yang
Tan, Xin-Qiu
Zhou, Xu-Guo
Shi, Xiao-Bin
Liu, Yong
Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus
title Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus
title_full Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus
title_fullStr Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus
title_full_unstemmed Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus
title_short Integrated Analysis of microRNA and mRNA Transcriptome Reveals the Molecular Mechanism of Solanum lycopersicum Response to Bemisia tabaci and Tomato chlorosis virus
title_sort integrated analysis of microrna and mrna transcriptome reveals the molecular mechanism of solanum lycopersicum response to bemisia tabaci and tomato chlorosis virus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258350/
https://www.ncbi.nlm.nih.gov/pubmed/34239512
http://dx.doi.org/10.3389/fmicb.2021.693574
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