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KIN17 promotes tumor metastasis by activating EMT signaling in luminal‐A breast cancer

BACKGROUND: Breast cancer (BC), the most common cause of cancer death in women, overtook lung cancer as the leading cause of cancer worldwide in 2020. Although many studies have proposed KIN17 as a biomarker of tumorigenesis in different cancer types, its role in tumor metastasis, particularly in BC...

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Autores principales: Huang, Qiyuan, Zahid, Kashif Rafiq, Chen, Jinsi, Pang, Xiangxiong, Zhong, Meifeng, Huang, Hongling, Pan, Weifeng, Yin, Jingxin, Raza, Umar, Zeng, Jiamin, Zhu, Xinhong, Zeng, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons Australia, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258367/
https://www.ncbi.nlm.nih.gov/pubmed/34008927
http://dx.doi.org/10.1111/1759-7714.14004
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author Huang, Qiyuan
Zahid, Kashif Rafiq
Chen, Jinsi
Pang, Xiangxiong
Zhong, Meifeng
Huang, Hongling
Pan, Weifeng
Yin, Jingxin
Raza, Umar
Zeng, Jiamin
Zhu, Xinhong
Zeng, Tao
author_facet Huang, Qiyuan
Zahid, Kashif Rafiq
Chen, Jinsi
Pang, Xiangxiong
Zhong, Meifeng
Huang, Hongling
Pan, Weifeng
Yin, Jingxin
Raza, Umar
Zeng, Jiamin
Zhu, Xinhong
Zeng, Tao
author_sort Huang, Qiyuan
collection PubMed
description BACKGROUND: Breast cancer (BC), the most common cause of cancer death in women, overtook lung cancer as the leading cause of cancer worldwide in 2020. Although many studies have proposed KIN17 as a biomarker of tumorigenesis in different cancer types, its role in tumor metastasis, particularly in BC metastasis, has been underexplored. This study aimed to explore the role of KIN17 in BC metastasis. METHODS: Survival analyses was performed to identify the association between KIN17 expression and BC patient survival in silico. Using lentivirus constructs, we developed bidirectional KIN17 expression (KD, knockdown; OE, overexpression) cellular models of luminal‐A (Lum‐A) breast cancer MCF‐7 cells. We performed in vitro wound healing, transwell with and without Matrigel assays, and in vivo tail‐vein metastasis assay to evaluate the migration and invasion abilities of MCF‐7 with stable KIN17 knockdown or overexpression. Western blotting was performed to compare the changes in protein expression. RESULTS: We found that KIN17 expression was associated with poor overall survival (OS), relapse‐free survival (RFS), distant metastasis‐free survival (DMFS) and post‐progression survival (PPS), particularly in Lum‐A breast cancer patients. Later, we found that KIN17 knockdown inhibited migration and invasion of MCF‐7 cells via regulating EMT‐associated signaling pathways in vitro and decreases metastatic spread of the disease in vivo. In contrast, KIN17 overexpression promoted migration and invasion of MCF‐7 cells in vitro and increased the metastatic spread of the disease in vivo. CONCLUSIONS: Overall, our findings provide preliminary data which suggests KIN17 of importance to target in metastatic Lum‐A patients.
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spelling pubmed-82583672021-07-12 KIN17 promotes tumor metastasis by activating EMT signaling in luminal‐A breast cancer Huang, Qiyuan Zahid, Kashif Rafiq Chen, Jinsi Pang, Xiangxiong Zhong, Meifeng Huang, Hongling Pan, Weifeng Yin, Jingxin Raza, Umar Zeng, Jiamin Zhu, Xinhong Zeng, Tao Thorac Cancer Original Articles BACKGROUND: Breast cancer (BC), the most common cause of cancer death in women, overtook lung cancer as the leading cause of cancer worldwide in 2020. Although many studies have proposed KIN17 as a biomarker of tumorigenesis in different cancer types, its role in tumor metastasis, particularly in BC metastasis, has been underexplored. This study aimed to explore the role of KIN17 in BC metastasis. METHODS: Survival analyses was performed to identify the association between KIN17 expression and BC patient survival in silico. Using lentivirus constructs, we developed bidirectional KIN17 expression (KD, knockdown; OE, overexpression) cellular models of luminal‐A (Lum‐A) breast cancer MCF‐7 cells. We performed in vitro wound healing, transwell with and without Matrigel assays, and in vivo tail‐vein metastasis assay to evaluate the migration and invasion abilities of MCF‐7 with stable KIN17 knockdown or overexpression. Western blotting was performed to compare the changes in protein expression. RESULTS: We found that KIN17 expression was associated with poor overall survival (OS), relapse‐free survival (RFS), distant metastasis‐free survival (DMFS) and post‐progression survival (PPS), particularly in Lum‐A breast cancer patients. Later, we found that KIN17 knockdown inhibited migration and invasion of MCF‐7 cells via regulating EMT‐associated signaling pathways in vitro and decreases metastatic spread of the disease in vivo. In contrast, KIN17 overexpression promoted migration and invasion of MCF‐7 cells in vitro and increased the metastatic spread of the disease in vivo. CONCLUSIONS: Overall, our findings provide preliminary data which suggests KIN17 of importance to target in metastatic Lum‐A patients. John Wiley & Sons Australia, Ltd 2021-05-19 2021-07 /pmc/articles/PMC8258367/ /pubmed/34008927 http://dx.doi.org/10.1111/1759-7714.14004 Text en © 2021 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Huang, Qiyuan
Zahid, Kashif Rafiq
Chen, Jinsi
Pang, Xiangxiong
Zhong, Meifeng
Huang, Hongling
Pan, Weifeng
Yin, Jingxin
Raza, Umar
Zeng, Jiamin
Zhu, Xinhong
Zeng, Tao
KIN17 promotes tumor metastasis by activating EMT signaling in luminal‐A breast cancer
title KIN17 promotes tumor metastasis by activating EMT signaling in luminal‐A breast cancer
title_full KIN17 promotes tumor metastasis by activating EMT signaling in luminal‐A breast cancer
title_fullStr KIN17 promotes tumor metastasis by activating EMT signaling in luminal‐A breast cancer
title_full_unstemmed KIN17 promotes tumor metastasis by activating EMT signaling in luminal‐A breast cancer
title_short KIN17 promotes tumor metastasis by activating EMT signaling in luminal‐A breast cancer
title_sort kin17 promotes tumor metastasis by activating emt signaling in luminal‐a breast cancer
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258367/
https://www.ncbi.nlm.nih.gov/pubmed/34008927
http://dx.doi.org/10.1111/1759-7714.14004
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