Cargando…
Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris
Cordyceps militaris is an entomopathogenic fungus producing a variety of bioactive compounds. To meet the huge demand for medicinal and edible products, industrialized fermentation of mycelia and cultivation of stromata have been widely developed in China. The content of bioactive metabolites of C....
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258390/ https://www.ncbi.nlm.nih.gov/pubmed/34239513 http://dx.doi.org/10.3389/fmicb.2021.698436 |
_version_ | 1783718496201342976 |
---|---|
author | Wang, Ying Yang, Zhanshan Bao, Dapeng Li, Bo Yin, Xin Wu, Yingying Chen, Hongyu Tang, Guirong Li, Nanyi Zou, Gen |
author_facet | Wang, Ying Yang, Zhanshan Bao, Dapeng Li, Bo Yin, Xin Wu, Yingying Chen, Hongyu Tang, Guirong Li, Nanyi Zou, Gen |
author_sort | Wang, Ying |
collection | PubMed |
description | Cordyceps militaris is an entomopathogenic fungus producing a variety of bioactive compounds. To meet the huge demand for medicinal and edible products, industrialized fermentation of mycelia and cultivation of stromata have been widely developed in China. The content of bioactive metabolites of C. militaris, such as cordycepin, is higher when cultivated on silkworm pupae than on rice or in broth. However, compared with other cultivation methods, C. militaris grows more slowly and accumulates less biomass. The hypoxic environment in pupa hemocoel is one of environmental factor which is not existed in other cultivation methods. It is suggested that hypoxia plays an important role on the growth and the synthesis of bioactive compounds in C. militaris. Here, we demonstrated that the distinct effects on the growth and synthesis of bioactive compounds employing different strategies of improving hypoxia adaption. The introduction of Vitreoscilla hemoglobin enhanced growth, biomass accumulation, and crude polysaccharides content of C. militaris. However, cordycepin production was decreased to 9–15% of the control group. Meanwhile, the yield of adenosine was increased significantly. Nonetheless, when the predicted bHLH transcription factor of sterol regulatory element binding proteins (SREBPs) was overexpressed in C. militaris to improve the hypoxia adaption of fungal cells, cordycepin content was significantly increased more than two-fold. These findings reveal the role of SREBPs on growth and bioactive compounds synthesis. And it also provides a scientific basis for rationally engineering strains and optimization strategies of air supply in cultivation and fermentation. |
format | Online Article Text |
id | pubmed-8258390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82583902021-07-07 Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris Wang, Ying Yang, Zhanshan Bao, Dapeng Li, Bo Yin, Xin Wu, Yingying Chen, Hongyu Tang, Guirong Li, Nanyi Zou, Gen Front Microbiol Microbiology Cordyceps militaris is an entomopathogenic fungus producing a variety of bioactive compounds. To meet the huge demand for medicinal and edible products, industrialized fermentation of mycelia and cultivation of stromata have been widely developed in China. The content of bioactive metabolites of C. militaris, such as cordycepin, is higher when cultivated on silkworm pupae than on rice or in broth. However, compared with other cultivation methods, C. militaris grows more slowly and accumulates less biomass. The hypoxic environment in pupa hemocoel is one of environmental factor which is not existed in other cultivation methods. It is suggested that hypoxia plays an important role on the growth and the synthesis of bioactive compounds in C. militaris. Here, we demonstrated that the distinct effects on the growth and synthesis of bioactive compounds employing different strategies of improving hypoxia adaption. The introduction of Vitreoscilla hemoglobin enhanced growth, biomass accumulation, and crude polysaccharides content of C. militaris. However, cordycepin production was decreased to 9–15% of the control group. Meanwhile, the yield of adenosine was increased significantly. Nonetheless, when the predicted bHLH transcription factor of sterol regulatory element binding proteins (SREBPs) was overexpressed in C. militaris to improve the hypoxia adaption of fungal cells, cordycepin content was significantly increased more than two-fold. These findings reveal the role of SREBPs on growth and bioactive compounds synthesis. And it also provides a scientific basis for rationally engineering strains and optimization strategies of air supply in cultivation and fermentation. Frontiers Media S.A. 2021-06-22 /pmc/articles/PMC8258390/ /pubmed/34239513 http://dx.doi.org/10.3389/fmicb.2021.698436 Text en Copyright © 2021 Wang, Yang, Bao, Li, Yin, Wu, Chen, Tang, Li and Zou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wang, Ying Yang, Zhanshan Bao, Dapeng Li, Bo Yin, Xin Wu, Yingying Chen, Hongyu Tang, Guirong Li, Nanyi Zou, Gen Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris |
title | Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris |
title_full | Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris |
title_fullStr | Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris |
title_full_unstemmed | Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris |
title_short | Improving Hypoxia Adaption Causes Distinct Effects on Growth and Bioactive Compounds Synthesis in an Entomopathogenic Fungus Cordyceps militaris |
title_sort | improving hypoxia adaption causes distinct effects on growth and bioactive compounds synthesis in an entomopathogenic fungus cordyceps militaris |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258390/ https://www.ncbi.nlm.nih.gov/pubmed/34239513 http://dx.doi.org/10.3389/fmicb.2021.698436 |
work_keys_str_mv | AT wangying improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT yangzhanshan improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT baodapeng improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT libo improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT yinxin improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT wuyingying improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT chenhongyu improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT tangguirong improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT linanyi improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris AT zougen improvinghypoxiaadaptioncausesdistincteffectsongrowthandbioactivecompoundssynthesisinanentomopathogenicfunguscordycepsmilitaris |