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Palindromic Sequence-Targeted (PST) PCR, Version 2: An Advanced Method for High-Throughput Targeted Gene Characterization and Transposon Display

Genome walking (GW), a strategy for capturing previously unsequenced DNA fragments that are in proximity to a known sequence tag, is currently predominantly based on PCR. Recently developed PCR-based methods allow for combining of sequence-specific primers with designed capturing primers capable of...

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Detalles Bibliográficos
Autores principales: Kalendar, Ruslan, Shustov, Alexandr V., Schulman, Alan H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258406/
https://www.ncbi.nlm.nih.gov/pubmed/34239528
http://dx.doi.org/10.3389/fpls.2021.691940