Cargando…
Oocyte specific lncRNA variant Rose influences oocyte and embryo development
Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expandi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258604/ https://www.ncbi.nlm.nih.gov/pubmed/34278057 http://dx.doi.org/10.1016/j.ncrna.2021.06.001 |
_version_ | 1783718530153185280 |
---|---|
author | Iyyappan, Rajan Aleshkina, Daria Zhu, Linkai Jiang, Zongliang Kinterova, Veronika Susor, Andrej |
author_facet | Iyyappan, Rajan Aleshkina, Daria Zhu, Linkai Jiang, Zongliang Kinterova, Veronika Susor, Andrej |
author_sort | Iyyappan, Rajan |
collection | PubMed |
description | Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expanding number of studies related to lncRNAs, their influence on oocyte physiology remains enigmatic. We found an oocyte specific antisense, long non-coding RNA, “Rose” (lncRNA in Oocyte Specifically Expressed) expressed in two variants containing two and three non-coding exons, respectively. Rose is localized in the nucleus of transcriptionally active oocyte and in embryo with polysomal occupancy in the cytoplasm. Experimental overexpression of Rose in fully grown oocyte did not show any differences in meiotic maturation. However, knocking down Rose resulted in abnormalities in oocyte cytokinesis and impaired preimplantation embryo development. In conclusion, we have identified an oocyte-specific maternal lncRNA that is essential for successful mammalian oocyte and embryo development. |
format | Online Article Text |
id | pubmed-8258604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-82586042021-07-16 Oocyte specific lncRNA variant Rose influences oocyte and embryo development Iyyappan, Rajan Aleshkina, Daria Zhu, Linkai Jiang, Zongliang Kinterova, Veronika Susor, Andrej Noncoding RNA Res Article Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expanding number of studies related to lncRNAs, their influence on oocyte physiology remains enigmatic. We found an oocyte specific antisense, long non-coding RNA, “Rose” (lncRNA in Oocyte Specifically Expressed) expressed in two variants containing two and three non-coding exons, respectively. Rose is localized in the nucleus of transcriptionally active oocyte and in embryo with polysomal occupancy in the cytoplasm. Experimental overexpression of Rose in fully grown oocyte did not show any differences in meiotic maturation. However, knocking down Rose resulted in abnormalities in oocyte cytokinesis and impaired preimplantation embryo development. In conclusion, we have identified an oocyte-specific maternal lncRNA that is essential for successful mammalian oocyte and embryo development. KeAi Publishing 2021-06-26 /pmc/articles/PMC8258604/ /pubmed/34278057 http://dx.doi.org/10.1016/j.ncrna.2021.06.001 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Iyyappan, Rajan Aleshkina, Daria Zhu, Linkai Jiang, Zongliang Kinterova, Veronika Susor, Andrej Oocyte specific lncRNA variant Rose influences oocyte and embryo development |
title | Oocyte specific lncRNA variant Rose influences oocyte and embryo development |
title_full | Oocyte specific lncRNA variant Rose influences oocyte and embryo development |
title_fullStr | Oocyte specific lncRNA variant Rose influences oocyte and embryo development |
title_full_unstemmed | Oocyte specific lncRNA variant Rose influences oocyte and embryo development |
title_short | Oocyte specific lncRNA variant Rose influences oocyte and embryo development |
title_sort | oocyte specific lncrna variant rose influences oocyte and embryo development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258604/ https://www.ncbi.nlm.nih.gov/pubmed/34278057 http://dx.doi.org/10.1016/j.ncrna.2021.06.001 |
work_keys_str_mv | AT iyyappanrajan oocytespecificlncrnavariantroseinfluencesoocyteandembryodevelopment AT aleshkinadaria oocytespecificlncrnavariantroseinfluencesoocyteandembryodevelopment AT zhulinkai oocytespecificlncrnavariantroseinfluencesoocyteandembryodevelopment AT jiangzongliang oocytespecificlncrnavariantroseinfluencesoocyteandembryodevelopment AT kinterovaveronika oocytespecificlncrnavariantroseinfluencesoocyteandembryodevelopment AT susorandrej oocytespecificlncrnavariantroseinfluencesoocyteandembryodevelopment |