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Oocyte specific lncRNA variant Rose influences oocyte and embryo development

Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expandi...

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Autores principales: Iyyappan, Rajan, Aleshkina, Daria, Zhu, Linkai, Jiang, Zongliang, Kinterova, Veronika, Susor, Andrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258604/
https://www.ncbi.nlm.nih.gov/pubmed/34278057
http://dx.doi.org/10.1016/j.ncrna.2021.06.001
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author Iyyappan, Rajan
Aleshkina, Daria
Zhu, Linkai
Jiang, Zongliang
Kinterova, Veronika
Susor, Andrej
author_facet Iyyappan, Rajan
Aleshkina, Daria
Zhu, Linkai
Jiang, Zongliang
Kinterova, Veronika
Susor, Andrej
author_sort Iyyappan, Rajan
collection PubMed
description Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expanding number of studies related to lncRNAs, their influence on oocyte physiology remains enigmatic. We found an oocyte specific antisense, long non-coding RNA, “Rose” (lncRNA in Oocyte Specifically Expressed) expressed in two variants containing two and three non-coding exons, respectively. Rose is localized in the nucleus of transcriptionally active oocyte and in embryo with polysomal occupancy in the cytoplasm. Experimental overexpression of Rose in fully grown oocyte did not show any differences in meiotic maturation. However, knocking down Rose resulted in abnormalities in oocyte cytokinesis and impaired preimplantation embryo development. In conclusion, we have identified an oocyte-specific maternal lncRNA that is essential for successful mammalian oocyte and embryo development.
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spelling pubmed-82586042021-07-16 Oocyte specific lncRNA variant Rose influences oocyte and embryo development Iyyappan, Rajan Aleshkina, Daria Zhu, Linkai Jiang, Zongliang Kinterova, Veronika Susor, Andrej Noncoding RNA Res Article Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expanding number of studies related to lncRNAs, their influence on oocyte physiology remains enigmatic. We found an oocyte specific antisense, long non-coding RNA, “Rose” (lncRNA in Oocyte Specifically Expressed) expressed in two variants containing two and three non-coding exons, respectively. Rose is localized in the nucleus of transcriptionally active oocyte and in embryo with polysomal occupancy in the cytoplasm. Experimental overexpression of Rose in fully grown oocyte did not show any differences in meiotic maturation. However, knocking down Rose resulted in abnormalities in oocyte cytokinesis and impaired preimplantation embryo development. In conclusion, we have identified an oocyte-specific maternal lncRNA that is essential for successful mammalian oocyte and embryo development. KeAi Publishing 2021-06-26 /pmc/articles/PMC8258604/ /pubmed/34278057 http://dx.doi.org/10.1016/j.ncrna.2021.06.001 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Iyyappan, Rajan
Aleshkina, Daria
Zhu, Linkai
Jiang, Zongliang
Kinterova, Veronika
Susor, Andrej
Oocyte specific lncRNA variant Rose influences oocyte and embryo development
title Oocyte specific lncRNA variant Rose influences oocyte and embryo development
title_full Oocyte specific lncRNA variant Rose influences oocyte and embryo development
title_fullStr Oocyte specific lncRNA variant Rose influences oocyte and embryo development
title_full_unstemmed Oocyte specific lncRNA variant Rose influences oocyte and embryo development
title_short Oocyte specific lncRNA variant Rose influences oocyte and embryo development
title_sort oocyte specific lncrna variant rose influences oocyte and embryo development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258604/
https://www.ncbi.nlm.nih.gov/pubmed/34278057
http://dx.doi.org/10.1016/j.ncrna.2021.06.001
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