Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium

Patients with chronic lung disease are vulnerable to getting severe diseases associated with SARS-CoV-2 infection. Here, we describe protocols for subculturing and differentiating primary normal human bronchial epithelial (NHBE) cells of patients with chronic obstructive lung disease. The differenti...

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Detalles Bibliográficos
Autores principales: Osan, Jaspreet Kaur, DeMontigny, Beth Ann, Mehedi, Masfique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259228/
https://www.ncbi.nlm.nih.gov/pubmed/34250510
http://dx.doi.org/10.1016/j.xpro.2021.100663
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author Osan, Jaspreet Kaur
DeMontigny, Beth Ann
Mehedi, Masfique
author_facet Osan, Jaspreet Kaur
DeMontigny, Beth Ann
Mehedi, Masfique
author_sort Osan, Jaspreet Kaur
collection PubMed
description Patients with chronic lung disease are vulnerable to getting severe diseases associated with SARS-CoV-2 infection. Here, we describe protocols for subculturing and differentiating primary normal human bronchial epithelial (NHBE) cells of patients with chronic obstructive lung disease. The differentiation of NHBE cells in air-liquid interface mimics an in vivo airway and provides an in vitro model for studying SARS-CoV-2 infection. We also describe a protocol for detecting proteins in the sectioned epithelium for detailing SARS-CoV-2 infection-induced pathobiology with a vertical view.
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spelling pubmed-82592282021-07-06 Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium Osan, Jaspreet Kaur DeMontigny, Beth Ann Mehedi, Masfique STAR Protoc Protocol Patients with chronic lung disease are vulnerable to getting severe diseases associated with SARS-CoV-2 infection. Here, we describe protocols for subculturing and differentiating primary normal human bronchial epithelial (NHBE) cells of patients with chronic obstructive lung disease. The differentiation of NHBE cells in air-liquid interface mimics an in vivo airway and provides an in vitro model for studying SARS-CoV-2 infection. We also describe a protocol for detecting proteins in the sectioned epithelium for detailing SARS-CoV-2 infection-induced pathobiology with a vertical view. Elsevier 2021-06-18 /pmc/articles/PMC8259228/ /pubmed/34250510 http://dx.doi.org/10.1016/j.xpro.2021.100663 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Osan, Jaspreet Kaur
DeMontigny, Beth Ann
Mehedi, Masfique
Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium
title Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium
title_full Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium
title_fullStr Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium
title_full_unstemmed Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium
title_short Immunohistochemistry for protein detection in PFA-fixed paraffin-embedded SARS-CoV-2-infected COPD airway epithelium
title_sort immunohistochemistry for protein detection in pfa-fixed paraffin-embedded sars-cov-2-infected copd airway epithelium
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259228/
https://www.ncbi.nlm.nih.gov/pubmed/34250510
http://dx.doi.org/10.1016/j.xpro.2021.100663
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AT mehedimasfique immunohistochemistryforproteindetectioninpfafixedparaffinembeddedsarscov2infectedcopdairwayepithelium

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