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Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis

Adipogenesis is regulated by genetic interactions, in which post-transcriptional regulation plays an important role. Staufen double-stranded RNA binding protein 1 (Staufen1 or STAU1) plays diverse roles in RNA processing and adipogenesis. Previously, we found that the downregulation of STAU1 affects...

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Autores principales: Liang, Xiaodi, Jiao, Yi, Gong, Xueli, Gu, Hao, Nuermaimaiti, Nuerbiye, Meng, Xuanyu, Liu, Dihui, Guan, Yaqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259723/
https://www.ncbi.nlm.nih.gov/pubmed/34224297
http://dx.doi.org/10.1080/21623945.2021.1948165
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author Liang, Xiaodi
Jiao, Yi
Gong, Xueli
Gu, Hao
Nuermaimaiti, Nuerbiye
Meng, Xuanyu
Liu, Dihui
Guan, Yaqun
author_facet Liang, Xiaodi
Jiao, Yi
Gong, Xueli
Gu, Hao
Nuermaimaiti, Nuerbiye
Meng, Xuanyu
Liu, Dihui
Guan, Yaqun
author_sort Liang, Xiaodi
collection PubMed
description Adipogenesis is regulated by genetic interactions, in which post-transcriptional regulation plays an important role. Staufen double-stranded RNA binding protein 1 (Staufen1 or STAU1) plays diverse roles in RNA processing and adipogenesis. Previously, we found that the downregulation of STAU1 affects the expression of fatty acid-binding protein 4 (FABP4) at the protein level but not at the mRNA level. This study aimed to determine the mechanism underlying the regulation of FABP4 expression by STAU1, explaining the inconsistency between FABP4 mRNA and protein levels. We used RNA interference, photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation, and an adeno-associated virus to examine the functions of STAU1 in adipogenesis. Our results indicate that STAU1 binds to the coding sequences of FABP4, thereby regulating the translation of FABP4 mRNA by unwinding the double-stranded structure. Furthermore, STAU1 mediates adipogenesis by regulating the secretion of free fatty acids. However, STAU1 knockdown decreases the fat weight/body weight ratio but does not affect the plasma triglyceride levels. These findings describe the mechanisms involved in STAU1-mediated regulation of FABP4 expression at the translational level during adipogenesis.
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spelling pubmed-82597232021-07-13 Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis Liang, Xiaodi Jiao, Yi Gong, Xueli Gu, Hao Nuermaimaiti, Nuerbiye Meng, Xuanyu Liu, Dihui Guan, Yaqun Adipocyte Research Paper Adipogenesis is regulated by genetic interactions, in which post-transcriptional regulation plays an important role. Staufen double-stranded RNA binding protein 1 (Staufen1 or STAU1) plays diverse roles in RNA processing and adipogenesis. Previously, we found that the downregulation of STAU1 affects the expression of fatty acid-binding protein 4 (FABP4) at the protein level but not at the mRNA level. This study aimed to determine the mechanism underlying the regulation of FABP4 expression by STAU1, explaining the inconsistency between FABP4 mRNA and protein levels. We used RNA interference, photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation, and an adeno-associated virus to examine the functions of STAU1 in adipogenesis. Our results indicate that STAU1 binds to the coding sequences of FABP4, thereby regulating the translation of FABP4 mRNA by unwinding the double-stranded structure. Furthermore, STAU1 mediates adipogenesis by regulating the secretion of free fatty acids. However, STAU1 knockdown decreases the fat weight/body weight ratio but does not affect the plasma triglyceride levels. These findings describe the mechanisms involved in STAU1-mediated regulation of FABP4 expression at the translational level during adipogenesis. Taylor & Francis 2021-07-05 /pmc/articles/PMC8259723/ /pubmed/34224297 http://dx.doi.org/10.1080/21623945.2021.1948165 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Liang, Xiaodi
Jiao, Yi
Gong, Xueli
Gu, Hao
Nuermaimaiti, Nuerbiye
Meng, Xuanyu
Liu, Dihui
Guan, Yaqun
Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis
title Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis
title_full Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis
title_fullStr Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis
title_full_unstemmed Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis
title_short Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis
title_sort staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mrna during adipogenesis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259723/
https://www.ncbi.nlm.nih.gov/pubmed/34224297
http://dx.doi.org/10.1080/21623945.2021.1948165
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