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Method development and characterisation of the low-molecular-weight peptidome of human wound fluids
The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260221/ https://www.ncbi.nlm.nih.gov/pubmed/34227939 http://dx.doi.org/10.7554/eLife.66876 |
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author | van der Plas, Mariena JA Cai, Jun Petrlova, Jitka Saleh, Karim Kjellström, Sven Schmidtchen, Artur |
author_facet | van der Plas, Mariena JA Cai, Jun Petrlova, Jitka Saleh, Karim Kjellström, Sven Schmidtchen, Artur |
author_sort | van der Plas, Mariena JA |
collection | PubMed |
description | The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection. |
format | Online Article Text |
id | pubmed-8260221 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-82602212021-07-07 Method development and characterisation of the low-molecular-weight peptidome of human wound fluids van der Plas, Mariena JA Cai, Jun Petrlova, Jitka Saleh, Karim Kjellström, Sven Schmidtchen, Artur eLife Biochemistry and Chemical Biology The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection. eLife Sciences Publications, Ltd 2021-07-06 /pmc/articles/PMC8260221/ /pubmed/34227939 http://dx.doi.org/10.7554/eLife.66876 Text en © 2021, van der Plas et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biochemistry and Chemical Biology van der Plas, Mariena JA Cai, Jun Petrlova, Jitka Saleh, Karim Kjellström, Sven Schmidtchen, Artur Method development and characterisation of the low-molecular-weight peptidome of human wound fluids |
title | Method development and characterisation of the low-molecular-weight peptidome of human wound fluids |
title_full | Method development and characterisation of the low-molecular-weight peptidome of human wound fluids |
title_fullStr | Method development and characterisation of the low-molecular-weight peptidome of human wound fluids |
title_full_unstemmed | Method development and characterisation of the low-molecular-weight peptidome of human wound fluids |
title_short | Method development and characterisation of the low-molecular-weight peptidome of human wound fluids |
title_sort | method development and characterisation of the low-molecular-weight peptidome of human wound fluids |
topic | Biochemistry and Chemical Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260221/ https://www.ncbi.nlm.nih.gov/pubmed/34227939 http://dx.doi.org/10.7554/eLife.66876 |
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