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Method development and characterisation of the low-molecular-weight peptidome of human wound fluids

The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using...

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Autores principales: van der Plas, Mariena JA, Cai, Jun, Petrlova, Jitka, Saleh, Karim, Kjellström, Sven, Schmidtchen, Artur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260221/
https://www.ncbi.nlm.nih.gov/pubmed/34227939
http://dx.doi.org/10.7554/eLife.66876
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author van der Plas, Mariena JA
Cai, Jun
Petrlova, Jitka
Saleh, Karim
Kjellström, Sven
Schmidtchen, Artur
author_facet van der Plas, Mariena JA
Cai, Jun
Petrlova, Jitka
Saleh, Karim
Kjellström, Sven
Schmidtchen, Artur
author_sort van der Plas, Mariena JA
collection PubMed
description The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection.
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spelling pubmed-82602212021-07-07 Method development and characterisation of the low-molecular-weight peptidome of human wound fluids van der Plas, Mariena JA Cai, Jun Petrlova, Jitka Saleh, Karim Kjellström, Sven Schmidtchen, Artur eLife Biochemistry and Chemical Biology The normal wound healing process is characterised by proteolytic events, whereas infection results in dysfunctional activations by endogenous and bacterial proteases. Peptides, downstream reporters of these proteolytic actions, could therefore serve as a promising tool for diagnosis of wounds. Using mass-spectrometry analyses, we here for the first time characterise the peptidome of human wound fluids. Sterile post-surgical wound fluids were found to contain a high degree of peptides in comparison to human plasma. Analyses of the peptidome from uninfected healing wounds and Staphylococcus aureus -infected wounds identify unique peptide patterns of various proteins, including coagulation and complement factors, proteases, and antiproteinases. Together, the work defines a workflow for analysis of peptides derived from wound fluids and demonstrates a proof-of-concept that such fluids can be used for analysis of qualitative differences of peptide patterns from larger patient cohorts, providing potential biomarkers for wound healing and infection. eLife Sciences Publications, Ltd 2021-07-06 /pmc/articles/PMC8260221/ /pubmed/34227939 http://dx.doi.org/10.7554/eLife.66876 Text en © 2021, van der Plas et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry and Chemical Biology
van der Plas, Mariena JA
Cai, Jun
Petrlova, Jitka
Saleh, Karim
Kjellström, Sven
Schmidtchen, Artur
Method development and characterisation of the low-molecular-weight peptidome of human wound fluids
title Method development and characterisation of the low-molecular-weight peptidome of human wound fluids
title_full Method development and characterisation of the low-molecular-weight peptidome of human wound fluids
title_fullStr Method development and characterisation of the low-molecular-weight peptidome of human wound fluids
title_full_unstemmed Method development and characterisation of the low-molecular-weight peptidome of human wound fluids
title_short Method development and characterisation of the low-molecular-weight peptidome of human wound fluids
title_sort method development and characterisation of the low-molecular-weight peptidome of human wound fluids
topic Biochemistry and Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260221/
https://www.ncbi.nlm.nih.gov/pubmed/34227939
http://dx.doi.org/10.7554/eLife.66876
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