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Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato
Genetic gain in potato is hampered by the heterozygous tetraploid genome of cultivated potato. Converting potato into a diploid inbred-line based F1-hybrid crop provides a promising route towards increased genetic gain. The introduction of a dominant S-locus inhibitor (Sli) gene into diploid potato...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260583/ https://www.ncbi.nlm.nih.gov/pubmed/34230471 http://dx.doi.org/10.1038/s41467-021-24267-6 |
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author | Eggers, Ernst-Jan van der Burgt, Ate van Heusden, Sjaak A. W. de Vries, Michiel E. Visser, Richard G. F. Bachem, Christian W. B. Lindhout, Pim |
author_facet | Eggers, Ernst-Jan van der Burgt, Ate van Heusden, Sjaak A. W. de Vries, Michiel E. Visser, Richard G. F. Bachem, Christian W. B. Lindhout, Pim |
author_sort | Eggers, Ernst-Jan |
collection | PubMed |
description | Genetic gain in potato is hampered by the heterozygous tetraploid genome of cultivated potato. Converting potato into a diploid inbred-line based F1-hybrid crop provides a promising route towards increased genetic gain. The introduction of a dominant S-locus inhibitor (Sli) gene into diploid potato germplasm allows efficient generation of self-fertilized seeds and thus the development of potato inbred lines. Little is known about the structure and function of the Sli locus. Here we describe the mapping of Sli to a 12.6 kb interval on chromosome 12 using a recombinant screen approach. One of two candidate genes present in this interval shows a unique sequence that is exclusively present in self-compatible lines. We describe an expression vector that converts self-incompatible genotypes into self-compatible and a CRISPR-Cas9 vector that converts SC genotypes into SI. The Sli gene encodes an F-box protein that is specifically expressed in pollen from self-compatible plants. A 533 bp insertion in the promotor of that gene leads to a gain of function mutation, which overcomes self-pollen rejection. |
format | Online Article Text |
id | pubmed-8260583 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82605832021-07-23 Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato Eggers, Ernst-Jan van der Burgt, Ate van Heusden, Sjaak A. W. de Vries, Michiel E. Visser, Richard G. F. Bachem, Christian W. B. Lindhout, Pim Nat Commun Article Genetic gain in potato is hampered by the heterozygous tetraploid genome of cultivated potato. Converting potato into a diploid inbred-line based F1-hybrid crop provides a promising route towards increased genetic gain. The introduction of a dominant S-locus inhibitor (Sli) gene into diploid potato germplasm allows efficient generation of self-fertilized seeds and thus the development of potato inbred lines. Little is known about the structure and function of the Sli locus. Here we describe the mapping of Sli to a 12.6 kb interval on chromosome 12 using a recombinant screen approach. One of two candidate genes present in this interval shows a unique sequence that is exclusively present in self-compatible lines. We describe an expression vector that converts self-incompatible genotypes into self-compatible and a CRISPR-Cas9 vector that converts SC genotypes into SI. The Sli gene encodes an F-box protein that is specifically expressed in pollen from self-compatible plants. A 533 bp insertion in the promotor of that gene leads to a gain of function mutation, which overcomes self-pollen rejection. Nature Publishing Group UK 2021-07-06 /pmc/articles/PMC8260583/ /pubmed/34230471 http://dx.doi.org/10.1038/s41467-021-24267-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Eggers, Ernst-Jan van der Burgt, Ate van Heusden, Sjaak A. W. de Vries, Michiel E. Visser, Richard G. F. Bachem, Christian W. B. Lindhout, Pim Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato |
title | Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato |
title_full | Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato |
title_fullStr | Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato |
title_full_unstemmed | Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato |
title_short | Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato |
title_sort | neofunctionalisation of the sli gene leads to self-compatibility and facilitates precision breeding in potato |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260583/ https://www.ncbi.nlm.nih.gov/pubmed/34230471 http://dx.doi.org/10.1038/s41467-021-24267-6 |
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