Biofilm addition improves sand strength over a wide range of saturations

Bio-mediated ground improvement is an attractive alternative to traditional admixtures for strength improvement of shallow surfaces because it is environmentally friendly. Since biofilms contain extracellular polymeric substances (EPS), they can be considered as an alternative to current technologies to improve soil strength. EPS containing biofilms are porous materials with charged surfaces, and therefore adsorption and capillary condensation can result in water retention. Currently, most of the literature work only tested soil improvement under the dry condition. Therefore, the influence of water retention by biofilms on the strength of improved soil remains unclear. Our goal is to evaluate the strength of a biofilm-enhanced sand over a wide range of saturations and explain the trends through the suction stress characteristic curve (SSCC), which quantifies interparticle adsorptive, capillary, and cementation forces as a function of saturation. We used homogenized anaerobic granule biofilms from an existing upflow anaerobic sludge blanket reactor and mixed it with sand to test the strength of a poorly-graded sand in a wide range of saturations, S (between 0.02 S and 0.74 S). We found that biofilm treatment of sand increases soil strength through cementation over a wide range of saturations and through adsorptive forces among sand, biofilm surfaces, and water molecules at low saturations (S < ~0.3). Our results suggested that homogenized biofilms mixed with sand can be used to improve the strength of sand over a wide range of saturations.