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Protocol for improving diffraction quality of leucyl-tRNA synthetase 1 with methylation and post-crystallization soaking and cooling in cryoprotectants

Leucyl-tRNA synthetase 1 (LARS1) synthesizes Leu-tRNA(Leu) for protein synthesis and plays an important role in mTORC1 activation by sensing intracellular leucine concentrations. Here, we describe a protocol for the purification, reductive methylation, binding affinity measurement by microscale ther...

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Detalles Bibliográficos
Autores principales:	Kim, Sulhee, Yoon, Ina, Kim, Sunghoon, Hwang, Kwang Yeon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260868/ https://www.ncbi.nlm.nih.gov/pubmed/34258600 http://dx.doi.org/10.1016/j.xpro.2021.100642

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