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Avaliação do kit Filaria Detect™ IgG4 produzido com o antígeno recombinante Wb123 para diagnóstico da filariose linfática no Brasil

The Global Programme to Eliminate Lymphatic Filariasis, launched by the World Health Organization in the year 2000, proposes the use of circulating filarial antigen tests as a diagnostic tool to assess and monitor initiatives to control filarial infection. However, despite a high sensitivity, these...

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Autores principales: Melo, Paula Fernanda A. S., Silva, Maria Almerice L., Oliveira, Maria Rosângela G., Araújo, Josué, Fernandes, Amanda, Diniz, George Tadeu N., Brandão, Eduardo, Rocha, Abraham, Maciel, Maria Amélia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Organización Panamericana de la Salud 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260883/
https://www.ncbi.nlm.nih.gov/pubmed/34249122
http://dx.doi.org/10.26633/RPSP.2021.87
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author Melo, Paula Fernanda A. S.
Silva, Maria Almerice L.
Oliveira, Maria Rosângela G.
Araújo, Josué
Fernandes, Amanda
Diniz, George Tadeu N.
Brandão, Eduardo
Rocha, Abraham
Maciel, Maria Amélia
author_facet Melo, Paula Fernanda A. S.
Silva, Maria Almerice L.
Oliveira, Maria Rosângela G.
Araújo, Josué
Fernandes, Amanda
Diniz, George Tadeu N.
Brandão, Eduardo
Rocha, Abraham
Maciel, Maria Amélia
author_sort Melo, Paula Fernanda A. S.
collection PubMed
description The Global Programme to Eliminate Lymphatic Filariasis, launched by the World Health Organization in the year 2000, proposes the use of circulating filarial antigen tests as a diagnostic tool to assess and monitor initiatives to control filarial infection. However, despite a high sensitivity, these tests are not efficient to detect infection at early stages, before worms have reached the adult stage. Considering this limitation, anti-filarial antibody testing has been suggested as an alternative, given that the antibodies produced against the larvae are detectable before the presence of circulating filarial antigen. The objective of the present study was to determine the diagnostic cut-off and the accuracy of the Filaria Detect™ IgG4 kit employing recombinant Wb123 antigen for diagnosis of lymphatic filariasis in Brazil. For that, we performed a diagnostic evaluation study in which 256 serum samples were analyzed: 79 (30.9%) obtained from microfilaremic individuals and 177 (60.1%) from amicrofilaremic individuals who tested negative with the Bm14 CELISA and Og4C3 ELISA immunologic tests. The ideal cutoff as well as the Filaria Detect™ IgG4 kit accuracy were determined based on ROC curve analyses, with an optical density of 0.239 identified as the cutoff with the best performance, with 81.0% sensitivity and 96.6% specificity. The results show that the Filaria Detect™ IgG4 kit is a promising tool for investigation and monitoring of areas undergoing mass drug administration for lymphatic filariasis.
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spelling pubmed-82608832021-07-08 Avaliação do kit Filaria Detect™ IgG4 produzido com o antígeno recombinante Wb123 para diagnóstico da filariose linfática no Brasil Melo, Paula Fernanda A. S. Silva, Maria Almerice L. Oliveira, Maria Rosângela G. Araújo, Josué Fernandes, Amanda Diniz, George Tadeu N. Brandão, Eduardo Rocha, Abraham Maciel, Maria Amélia Rev Panam Salud Publica Comunicação Breve The Global Programme to Eliminate Lymphatic Filariasis, launched by the World Health Organization in the year 2000, proposes the use of circulating filarial antigen tests as a diagnostic tool to assess and monitor initiatives to control filarial infection. However, despite a high sensitivity, these tests are not efficient to detect infection at early stages, before worms have reached the adult stage. Considering this limitation, anti-filarial antibody testing has been suggested as an alternative, given that the antibodies produced against the larvae are detectable before the presence of circulating filarial antigen. The objective of the present study was to determine the diagnostic cut-off and the accuracy of the Filaria Detect™ IgG4 kit employing recombinant Wb123 antigen for diagnosis of lymphatic filariasis in Brazil. For that, we performed a diagnostic evaluation study in which 256 serum samples were analyzed: 79 (30.9%) obtained from microfilaremic individuals and 177 (60.1%) from amicrofilaremic individuals who tested negative with the Bm14 CELISA and Og4C3 ELISA immunologic tests. The ideal cutoff as well as the Filaria Detect™ IgG4 kit accuracy were determined based on ROC curve analyses, with an optical density of 0.239 identified as the cutoff with the best performance, with 81.0% sensitivity and 96.6% specificity. The results show that the Filaria Detect™ IgG4 kit is a promising tool for investigation and monitoring of areas undergoing mass drug administration for lymphatic filariasis. Organización Panamericana de la Salud 2021-07-08 /pmc/articles/PMC8260883/ /pubmed/34249122 http://dx.doi.org/10.26633/RPSP.2021.87 Text en https://creativecommons.org/licenses/by/2.5/Este é um artigo de acesso aberto distribuído sob os termos da Licença Creative Commons Attribution-NonCommercial-NoDerivs 3.0 IGO, que permite o uso, distribuição e reprodução em qualquer meio, desde que o trabalho original seja devidamente citado. Não são permitidas modificações ou uso comercial dos artigos. Em qualquer reprodução do artigo, não deve haver nenhuma sugestão de que a OPAS ou o artigo avaliem qualquer organização ou produtos específicos. Não é permitido o uso do logotipo da OPAS. Este aviso deve ser preservado juntamente com o URL original do artigo. Crédito do logotipo e texto em acesso aberto: PLoS, sob licença Creative Commons Attribution-Share Alike 3.0 Unported
spellingShingle Comunicação Breve
Melo, Paula Fernanda A. S.
Silva, Maria Almerice L.
Oliveira, Maria Rosângela G.
Araújo, Josué
Fernandes, Amanda
Diniz, George Tadeu N.
Brandão, Eduardo
Rocha, Abraham
Maciel, Maria Amélia
Avaliação do kit Filaria Detect™ IgG4 produzido com o antígeno recombinante Wb123 para diagnóstico da filariose linfática no Brasil
title Avaliação do kit Filaria Detect™ IgG4 produzido com o antígeno recombinante Wb123 para diagnóstico da filariose linfática no Brasil
title_full Avaliação do kit Filaria Detect™ IgG4 produzido com o antígeno recombinante Wb123 para diagnóstico da filariose linfática no Brasil
title_fullStr Avaliação do kit Filaria Detect™ IgG4 produzido com o antígeno recombinante Wb123 para diagnóstico da filariose linfática no Brasil
title_full_unstemmed Avaliação do kit Filaria Detect™ IgG4 produzido com o antígeno recombinante Wb123 para diagnóstico da filariose linfática no Brasil
title_short Avaliação do kit Filaria Detect™ IgG4 produzido com o antígeno recombinante Wb123 para diagnóstico da filariose linfática no Brasil
title_sort avaliação do kit filaria detect™ igg4 produzido com o antígeno recombinante wb123 para diagnóstico da filariose linfática no brasil
topic Comunicação Breve
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260883/
https://www.ncbi.nlm.nih.gov/pubmed/34249122
http://dx.doi.org/10.26633/RPSP.2021.87
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