Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets

Platelet preparations are commonly used in the clinic in combination with mesenchymal stem cells (MSCs) to improve their wound healing capacity and optimize their therapeutic efficacy following their delivery into diseased tissues. To investigate the mechanisms by which platelets enhance the repair...

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Detalles Bibliográficos
Autores principales: Levoux, Jennyfer, Lafuste, Peggy, Rodriguez, Anne-Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261014/
https://www.ncbi.nlm.nih.gov/pubmed/34278336
http://dx.doi.org/10.1016/j.xpro.2021.100650
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author Levoux, Jennyfer
Lafuste, Peggy
Rodriguez, Anne-Marie
author_facet Levoux, Jennyfer
Lafuste, Peggy
Rodriguez, Anne-Marie
author_sort Levoux, Jennyfer
collection PubMed
description Platelet preparations are commonly used in the clinic in combination with mesenchymal stem cells (MSCs) to improve their wound healing capacity and optimize their therapeutic efficacy following their delivery into diseased tissues. To investigate the mechanisms by which platelets enhance the repair properties of MSCs, we detail a protocol using a humanized mouse model for excisional wounds to study by reverse transcription real-time PCR whether human platelets alter the therapeutic efficacy of grafted human MSCs. For complete details on the use and execution of this protocol, please refer to Levoux et al. (2021).
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spelling pubmed-82610142021-07-16 Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets Levoux, Jennyfer Lafuste, Peggy Rodriguez, Anne-Marie STAR Protoc Protocol Platelet preparations are commonly used in the clinic in combination with mesenchymal stem cells (MSCs) to improve their wound healing capacity and optimize their therapeutic efficacy following their delivery into diseased tissues. To investigate the mechanisms by which platelets enhance the repair properties of MSCs, we detail a protocol using a humanized mouse model for excisional wounds to study by reverse transcription real-time PCR whether human platelets alter the therapeutic efficacy of grafted human MSCs. For complete details on the use and execution of this protocol, please refer to Levoux et al. (2021). Elsevier 2021-07-03 /pmc/articles/PMC8261014/ /pubmed/34278336 http://dx.doi.org/10.1016/j.xpro.2021.100650 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Levoux, Jennyfer
Lafuste, Peggy
Rodriguez, Anne-Marie
Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets
title Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets
title_full Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets
title_fullStr Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets
title_full_unstemmed Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets
title_short Transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets
title_sort transcriptional analysis of mouse wounds grafted with human mesenchymal stem cells and platelets
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261014/
https://www.ncbi.nlm.nih.gov/pubmed/34278336
http://dx.doi.org/10.1016/j.xpro.2021.100650
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