Cargando…
The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance
While drought severely affects plant growth and crop production, the molecular mechanisms of the drought response of plants remain unclear. In this study, we demonstrated for the first time the effect of the pseudo-protease AtFtsHi3 of Arabidopsis thaliana on overall plant growth and in drought tole...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261292/ https://www.ncbi.nlm.nih.gov/pubmed/34249066 http://dx.doi.org/10.3389/fpls.2021.694727 |
_version_ | 1783718984981413888 |
---|---|
author | Mishra, Laxmi S. Mishra, Sanatkumar Caddell, Daniel F. Coleman-Derr, Devin Funk, Christiane |
author_facet | Mishra, Laxmi S. Mishra, Sanatkumar Caddell, Daniel F. Coleman-Derr, Devin Funk, Christiane |
author_sort | Mishra, Laxmi S. |
collection | PubMed |
description | While drought severely affects plant growth and crop production, the molecular mechanisms of the drought response of plants remain unclear. In this study, we demonstrated for the first time the effect of the pseudo-protease AtFtsHi3 of Arabidopsis thaliana on overall plant growth and in drought tolerance. An AtFTSHi3 knock-down mutant [ftshi3-1(kd)] displayed a pale-green phenotype with lower photosynthetic efficiency and Darwinian fitness compared to wild type (Wt). An observed delay in seed germination of ftshi3-1(kd) was attributed to overaccumulation of abscisic acid (ABA); ftshi3-1(kd) seedlings showed partial sensitivity to exogenous ABA. Being exposed to similar severity of soil drying, ftshi3-1(kd) was drought-tolerant up to 20 days after the last irrigation, while wild type plants wilted after 12 days. Leaves of ftshi3-1(kd) contained reduced stomata size, density, and a smaller stomatic aperture. During drought stress, ftshi3-1(kd) showed lowered stomatal conductance, increased intrinsic water-use efficiency (WUEi), and slower stress acclimation. Expression levels of ABA-responsive genes were higher in leaves of ftshi3-1(kd) than Wt; DREB1A, but not DREB2A, was significantly upregulated during drought. However, although ftshi3-1(kd) displayed a drought-tolerant phenotype in aboveground tissue, the root-associated bacterial community responded to drought. |
format | Online Article Text |
id | pubmed-8261292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82612922021-07-08 The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance Mishra, Laxmi S. Mishra, Sanatkumar Caddell, Daniel F. Coleman-Derr, Devin Funk, Christiane Front Plant Sci Plant Science While drought severely affects plant growth and crop production, the molecular mechanisms of the drought response of plants remain unclear. In this study, we demonstrated for the first time the effect of the pseudo-protease AtFtsHi3 of Arabidopsis thaliana on overall plant growth and in drought tolerance. An AtFTSHi3 knock-down mutant [ftshi3-1(kd)] displayed a pale-green phenotype with lower photosynthetic efficiency and Darwinian fitness compared to wild type (Wt). An observed delay in seed germination of ftshi3-1(kd) was attributed to overaccumulation of abscisic acid (ABA); ftshi3-1(kd) seedlings showed partial sensitivity to exogenous ABA. Being exposed to similar severity of soil drying, ftshi3-1(kd) was drought-tolerant up to 20 days after the last irrigation, while wild type plants wilted after 12 days. Leaves of ftshi3-1(kd) contained reduced stomata size, density, and a smaller stomatic aperture. During drought stress, ftshi3-1(kd) showed lowered stomatal conductance, increased intrinsic water-use efficiency (WUEi), and slower stress acclimation. Expression levels of ABA-responsive genes were higher in leaves of ftshi3-1(kd) than Wt; DREB1A, but not DREB2A, was significantly upregulated during drought. However, although ftshi3-1(kd) displayed a drought-tolerant phenotype in aboveground tissue, the root-associated bacterial community responded to drought. Frontiers Media S.A. 2021-06-23 /pmc/articles/PMC8261292/ /pubmed/34249066 http://dx.doi.org/10.3389/fpls.2021.694727 Text en Copyright © 2021 Mishra, Mishra, Caddell, Coleman-Derr and Funk. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Mishra, Laxmi S. Mishra, Sanatkumar Caddell, Daniel F. Coleman-Derr, Devin Funk, Christiane The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance |
title | The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance |
title_full | The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance |
title_fullStr | The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance |
title_full_unstemmed | The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance |
title_short | The Plastid-Localized AtFtsHi3 Pseudo-Protease of Arabidopsis thaliana Has an Impact on Plant Growth and Drought Tolerance |
title_sort | plastid-localized atftshi3 pseudo-protease of arabidopsis thaliana has an impact on plant growth and drought tolerance |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261292/ https://www.ncbi.nlm.nih.gov/pubmed/34249066 http://dx.doi.org/10.3389/fpls.2021.694727 |
work_keys_str_mv | AT mishralaxmis theplastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT mishrasanatkumar theplastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT caddelldanielf theplastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT colemanderrdevin theplastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT funkchristiane theplastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT mishralaxmis plastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT mishrasanatkumar plastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT caddelldanielf plastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT colemanderrdevin plastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance AT funkchristiane plastidlocalizedatftshi3pseudoproteaseofarabidopsisthalianahasanimpactonplantgrowthanddroughttolerance |