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Ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors
BACKGROUND: Real-time multispectral imaging (rMSI) simultaneously provides white light (WL), photodynamic diagnosis (PDD) images, and a real-time fusion of both. It may improve the detection of bladder tumors. However, rMSI has not been used for transurethral biopsy or resection so far. The aim of t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261439/ https://www.ncbi.nlm.nih.gov/pubmed/34295724 http://dx.doi.org/10.21037/tau-20-1372 |
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author | Grüne, Britta Rother, Jan Waldbillig, Frank Chellappan, Ganapathy Meessen, Sabine Grychtol, Bartłomiej Deliolanis, Nikolaos C. Bolenz, Christian Kriegmair, Maximilian C. |
author_facet | Grüne, Britta Rother, Jan Waldbillig, Frank Chellappan, Ganapathy Meessen, Sabine Grychtol, Bartłomiej Deliolanis, Nikolaos C. Bolenz, Christian Kriegmair, Maximilian C. |
author_sort | Grüne, Britta |
collection | PubMed |
description | BACKGROUND: Real-time multispectral imaging (rMSI) simultaneously provides white light (WL), photodynamic diagnosis (PDD) images, and a real-time fusion of both. It may improve the detection of bladder tumors. However, rMSI has not been used for transurethral biopsy or resection so far. The aim of this ex vivo study was to test the feasibility of bladder tumor biopsies using the rMSI system and compare it to a conventional endoscopic system. METHODS: A 3D printed rigid bladder phantom was equipped with small and flat (5 mm × 1 mm) mock-bladder-tumors made of silicone and fluorescent Qdots655 (Thermo Fisher Scientific, Germany). Urologists (n=15) were asked to perform a rigid cystoscopy and biopsy of all identified lesions (n=6) using a prototype rMSI system and the Image1 S system (Karl Storz, Tuttlingen). Success rate and completion time were measured. The image quality of both systems and the usability of the rMSI system according to the system usability scale (SUS) were evaluated with a task-specific questionnaire. RESULTS: Tumor detection and biopsy rate were 100% (90/90) for the rMSI system and 98.9% (89/90) for the Image1 S system (P=0.3). The biopsy completion time did not differ significantly between the systems (P=0.48). Differentiation between healthy and suspect mucosa with the rMSI system was rated as comparable to the Image1 S system by 53% of surgeons and as better by 33% of the surgeons. The median SUS score for the rMSI system was 87.5%. CONCLUSIONS: Accurate transurethral biopsies are feasible with the rMSI system. Furthermore, the rMSI system has an excellent SUS. This study paves the way to the first in-human transurethral resections of bladder tumors (TUR-B) using rMSI technology. |
format | Online Article Text |
id | pubmed-8261439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-82614392021-07-21 Ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors Grüne, Britta Rother, Jan Waldbillig, Frank Chellappan, Ganapathy Meessen, Sabine Grychtol, Bartłomiej Deliolanis, Nikolaos C. Bolenz, Christian Kriegmair, Maximilian C. Transl Androl Urol Original Article BACKGROUND: Real-time multispectral imaging (rMSI) simultaneously provides white light (WL), photodynamic diagnosis (PDD) images, and a real-time fusion of both. It may improve the detection of bladder tumors. However, rMSI has not been used for transurethral biopsy or resection so far. The aim of this ex vivo study was to test the feasibility of bladder tumor biopsies using the rMSI system and compare it to a conventional endoscopic system. METHODS: A 3D printed rigid bladder phantom was equipped with small and flat (5 mm × 1 mm) mock-bladder-tumors made of silicone and fluorescent Qdots655 (Thermo Fisher Scientific, Germany). Urologists (n=15) were asked to perform a rigid cystoscopy and biopsy of all identified lesions (n=6) using a prototype rMSI system and the Image1 S system (Karl Storz, Tuttlingen). Success rate and completion time were measured. The image quality of both systems and the usability of the rMSI system according to the system usability scale (SUS) were evaluated with a task-specific questionnaire. RESULTS: Tumor detection and biopsy rate were 100% (90/90) for the rMSI system and 98.9% (89/90) for the Image1 S system (P=0.3). The biopsy completion time did not differ significantly between the systems (P=0.48). Differentiation between healthy and suspect mucosa with the rMSI system was rated as comparable to the Image1 S system by 53% of surgeons and as better by 33% of the surgeons. The median SUS score for the rMSI system was 87.5%. CONCLUSIONS: Accurate transurethral biopsies are feasible with the rMSI system. Furthermore, the rMSI system has an excellent SUS. This study paves the way to the first in-human transurethral resections of bladder tumors (TUR-B) using rMSI technology. AME Publishing Company 2021-06 /pmc/articles/PMC8261439/ /pubmed/34295724 http://dx.doi.org/10.21037/tau-20-1372 Text en 2021 Translational Andrology and Urology. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Grüne, Britta Rother, Jan Waldbillig, Frank Chellappan, Ganapathy Meessen, Sabine Grychtol, Bartłomiej Deliolanis, Nikolaos C. Bolenz, Christian Kriegmair, Maximilian C. Ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors |
title | Ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors |
title_full | Ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors |
title_fullStr | Ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors |
title_full_unstemmed | Ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors |
title_short | Ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors |
title_sort | ex vivo validation of a real-time multispectral endoscopic system for the detection and biopsy of bladder tumors |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261439/ https://www.ncbi.nlm.nih.gov/pubmed/34295724 http://dx.doi.org/10.21037/tau-20-1372 |
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