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Production of Rainbow Colorants by Metabolically Engineered Escherichia coli

There has been much interest in producing natural colorants to replace synthetic colorants of health concerns. Escherichia coli has been employed to produce natural colorants including carotenoids, indigo, anthocyanins, and violacein. However, production of natural green and navy colorants has not b...

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Autores principales: Yang, Dongsoo, Park, Seon Young, Lee, Sang Yup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261500/
https://www.ncbi.nlm.nih.gov/pubmed/34032018
http://dx.doi.org/10.1002/advs.202100743
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author Yang, Dongsoo
Park, Seon Young
Lee, Sang Yup
author_facet Yang, Dongsoo
Park, Seon Young
Lee, Sang Yup
author_sort Yang, Dongsoo
collection PubMed
description There has been much interest in producing natural colorants to replace synthetic colorants of health concerns. Escherichia coli has been employed to produce natural colorants including carotenoids, indigo, anthocyanins, and violacein. However, production of natural green and navy colorants has not been reported. Many natural products are hydrophobic, which are accumulated inside or on the cell membrane. This causes cell growth limitation and consequently reduces production of target chemicals. Here, integrated membrane engineering strategies are reported for the enhanced production of rainbow colorants—three carotenoids and four violacein derivatives—as representative hydrophobic natural products in E. coli. By integration of systems metabolic engineering, cell morphology engineering, inner‐ and outer‐membrane vesicle formation, and fermentation optimization, production of rainbow colorants are significantly enhanced to 322 mg L(–1) of astaxanthin (red), 343 mg L(–1) of β‐carotene (orange), 218 mg L(–1) of zeaxanthin (yellow), 1.42 g L(–1) of proviolacein (green), 0.844 g L(–1) of prodeoxyviolacein (blue), 6.19 g L(–1) of violacein (navy), and 11.26 g L(–1) of deoxyviolacein (purple). The membrane engineering strategies reported here are generally applicable to microbial production of a broader range of hydrophobic natural products, contributing to food, cosmetic, chemical, and pharmaceutical industries.
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spelling pubmed-82615002021-07-12 Production of Rainbow Colorants by Metabolically Engineered Escherichia coli Yang, Dongsoo Park, Seon Young Lee, Sang Yup Adv Sci (Weinh) Research Articles There has been much interest in producing natural colorants to replace synthetic colorants of health concerns. Escherichia coli has been employed to produce natural colorants including carotenoids, indigo, anthocyanins, and violacein. However, production of natural green and navy colorants has not been reported. Many natural products are hydrophobic, which are accumulated inside or on the cell membrane. This causes cell growth limitation and consequently reduces production of target chemicals. Here, integrated membrane engineering strategies are reported for the enhanced production of rainbow colorants—three carotenoids and four violacein derivatives—as representative hydrophobic natural products in E. coli. By integration of systems metabolic engineering, cell morphology engineering, inner‐ and outer‐membrane vesicle formation, and fermentation optimization, production of rainbow colorants are significantly enhanced to 322 mg L(–1) of astaxanthin (red), 343 mg L(–1) of β‐carotene (orange), 218 mg L(–1) of zeaxanthin (yellow), 1.42 g L(–1) of proviolacein (green), 0.844 g L(–1) of prodeoxyviolacein (blue), 6.19 g L(–1) of violacein (navy), and 11.26 g L(–1) of deoxyviolacein (purple). The membrane engineering strategies reported here are generally applicable to microbial production of a broader range of hydrophobic natural products, contributing to food, cosmetic, chemical, and pharmaceutical industries. John Wiley and Sons Inc. 2021-05-25 /pmc/articles/PMC8261500/ /pubmed/34032018 http://dx.doi.org/10.1002/advs.202100743 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Yang, Dongsoo
Park, Seon Young
Lee, Sang Yup
Production of Rainbow Colorants by Metabolically Engineered Escherichia coli
title Production of Rainbow Colorants by Metabolically Engineered Escherichia coli
title_full Production of Rainbow Colorants by Metabolically Engineered Escherichia coli
title_fullStr Production of Rainbow Colorants by Metabolically Engineered Escherichia coli
title_full_unstemmed Production of Rainbow Colorants by Metabolically Engineered Escherichia coli
title_short Production of Rainbow Colorants by Metabolically Engineered Escherichia coli
title_sort production of rainbow colorants by metabolically engineered escherichia coli
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261500/
https://www.ncbi.nlm.nih.gov/pubmed/34032018
http://dx.doi.org/10.1002/advs.202100743
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