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Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4
Plasma LDL is produced from catabolism of VLDL and cleared from circulation mainly via the hepatic LDL receptor (LDLR). Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes LDLR degradation, increasing plasma LDL-C levels. Circulating PCSK9 is mainly secreted by the liver, whereas VLDL is...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261665/ https://www.ncbi.nlm.nih.gov/pubmed/34118252 http://dx.doi.org/10.1016/j.jlr.2021.100091 |
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author | Wang, Bingxiang Shen, Yishi Zhai, Lei Xia, Xiaodan Gu, Hong-mei Wang, Maggie Zhao, Yongfang Chang, Xiaole Alabi, Adekunle Xing, Sijie Deng, Shijun Liu, Boyan Wang, Guiqing Qin, Shucun Zhang, Da-wei |
author_facet | Wang, Bingxiang Shen, Yishi Zhai, Lei Xia, Xiaodan Gu, Hong-mei Wang, Maggie Zhao, Yongfang Chang, Xiaole Alabi, Adekunle Xing, Sijie Deng, Shijun Liu, Boyan Wang, Guiqing Qin, Shucun Zhang, Da-wei |
author_sort | Wang, Bingxiang |
collection | PubMed |
description | Plasma LDL is produced from catabolism of VLDL and cleared from circulation mainly via the hepatic LDL receptor (LDLR). Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes LDLR degradation, increasing plasma LDL-C levels. Circulating PCSK9 is mainly secreted by the liver, whereas VLDL is exclusively secreted by hepatocytes. However, the mechanism regulating their secretion is not completely understood. Surfeit 4 (Surf4) is a cargo receptor localized in the ER membrane. It recruits cargos into coat protein complex II vesicles to facilitate their secretion. Here, we investigated the role of Surf4 in VLDL and PCSK9 secretion. We generated Surf4 liver-specific knockout mice and found that knockout of Surf4 did not affect PCSK9 secretion, whereas it significantly reduced plasma levels of cholesterol, triglyceride, and lipid-binding protein apolipoprotein B (apoB). In cultured human hepatocytes, Surf4 coimmunoprecipitated and colocalized with apolipoprotein B100, and Surf4 silencing reduced secretion of apolipoprotein B100. Furthermore, knockdown of Surf4 in LDLR knockout (Ldlr(−/−)) mice significantly reduced triglyceride secretion, plasma levels of apoB and non-HDL-C, and the development of atherosclerosis. However, Surf4 liver-specific knockout mice and Surf4 knockdown in Ldlr(−/−) mice displayed similar levels of liver lipids and plasma alanine aminotransferase activity as control mice, indicating that inhibition of Surf4 does not cause notable liver damage. Expression of stearoyl-CoA desaturase-1 was also reduced in the liver of these mice, suggesting a reduction in de novo lipogenesis. In summary, hepatic deficiency of Surf4 reduced VLDL secretion and the development of atherosclerosis but did not cause significant hepatic lipid accumulation or liver damage. |
format | Online Article Text |
id | pubmed-8261665 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-82616652021-07-16 Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4 Wang, Bingxiang Shen, Yishi Zhai, Lei Xia, Xiaodan Gu, Hong-mei Wang, Maggie Zhao, Yongfang Chang, Xiaole Alabi, Adekunle Xing, Sijie Deng, Shijun Liu, Boyan Wang, Guiqing Qin, Shucun Zhang, Da-wei J Lipid Res Research Article Plasma LDL is produced from catabolism of VLDL and cleared from circulation mainly via the hepatic LDL receptor (LDLR). Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes LDLR degradation, increasing plasma LDL-C levels. Circulating PCSK9 is mainly secreted by the liver, whereas VLDL is exclusively secreted by hepatocytes. However, the mechanism regulating their secretion is not completely understood. Surfeit 4 (Surf4) is a cargo receptor localized in the ER membrane. It recruits cargos into coat protein complex II vesicles to facilitate their secretion. Here, we investigated the role of Surf4 in VLDL and PCSK9 secretion. We generated Surf4 liver-specific knockout mice and found that knockout of Surf4 did not affect PCSK9 secretion, whereas it significantly reduced plasma levels of cholesterol, triglyceride, and lipid-binding protein apolipoprotein B (apoB). In cultured human hepatocytes, Surf4 coimmunoprecipitated and colocalized with apolipoprotein B100, and Surf4 silencing reduced secretion of apolipoprotein B100. Furthermore, knockdown of Surf4 in LDLR knockout (Ldlr(−/−)) mice significantly reduced triglyceride secretion, plasma levels of apoB and non-HDL-C, and the development of atherosclerosis. However, Surf4 liver-specific knockout mice and Surf4 knockdown in Ldlr(−/−) mice displayed similar levels of liver lipids and plasma alanine aminotransferase activity as control mice, indicating that inhibition of Surf4 does not cause notable liver damage. Expression of stearoyl-CoA desaturase-1 was also reduced in the liver of these mice, suggesting a reduction in de novo lipogenesis. In summary, hepatic deficiency of Surf4 reduced VLDL secretion and the development of atherosclerosis but did not cause significant hepatic lipid accumulation or liver damage. American Society for Biochemistry and Molecular Biology 2021-06-09 /pmc/articles/PMC8261665/ /pubmed/34118252 http://dx.doi.org/10.1016/j.jlr.2021.100091 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Wang, Bingxiang Shen, Yishi Zhai, Lei Xia, Xiaodan Gu, Hong-mei Wang, Maggie Zhao, Yongfang Chang, Xiaole Alabi, Adekunle Xing, Sijie Deng, Shijun Liu, Boyan Wang, Guiqing Qin, Shucun Zhang, Da-wei Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4 |
title | Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4 |
title_full | Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4 |
title_fullStr | Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4 |
title_full_unstemmed | Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4 |
title_short | Atherosclerosis-associated hepatic secretion of VLDL but not PCSK9 is dependent on cargo receptor protein Surf4 |
title_sort | atherosclerosis-associated hepatic secretion of vldl but not pcsk9 is dependent on cargo receptor protein surf4 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261665/ https://www.ncbi.nlm.nih.gov/pubmed/34118252 http://dx.doi.org/10.1016/j.jlr.2021.100091 |
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