Mechanical characterization of base analogue modified nucleic acids by force spectroscopy

We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study high...

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Detalles Bibliográficos
Autores principales: Sundar Rajan, Vinoth, Viader-Godoy, Xavier, Lin, Yii-Lih, Dutta, Uttama, Ritort, Felix, Westerlund, Fredrik, Wilhelmsson, L. Marcus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261857/
https://www.ncbi.nlm.nih.gov/pubmed/34180930
http://dx.doi.org/10.1039/d1cp01985f
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author Sundar Rajan, Vinoth
Viader-Godoy, Xavier
Lin, Yii-Lih
Dutta, Uttama
Ritort, Felix
Westerlund, Fredrik
Wilhelmsson, L. Marcus
author_facet Sundar Rajan, Vinoth
Viader-Godoy, Xavier
Lin, Yii-Lih
Dutta, Uttama
Ritort, Felix
Westerlund, Fredrik
Wilhelmsson, L. Marcus
author_sort Sundar Rajan, Vinoth
collection PubMed
description We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy.
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spelling pubmed-82618572021-07-16 Mechanical characterization of base analogue modified nucleic acids by force spectroscopy Sundar Rajan, Vinoth Viader-Godoy, Xavier Lin, Yii-Lih Dutta, Uttama Ritort, Felix Westerlund, Fredrik Wilhelmsson, L. Marcus Phys Chem Chem Phys Chemistry We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy. The Royal Society of Chemistry 2021-06-22 /pmc/articles/PMC8261857/ /pubmed/34180930 http://dx.doi.org/10.1039/d1cp01985f Text en This journal is © the Owner Societies https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Sundar Rajan, Vinoth
Viader-Godoy, Xavier
Lin, Yii-Lih
Dutta, Uttama
Ritort, Felix
Westerlund, Fredrik
Wilhelmsson, L. Marcus
Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
title Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
title_full Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
title_fullStr Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
title_full_unstemmed Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
title_short Mechanical characterization of base analogue modified nucleic acids by force spectroscopy
title_sort mechanical characterization of base analogue modified nucleic acids by force spectroscopy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261857/
https://www.ncbi.nlm.nih.gov/pubmed/34180930
http://dx.doi.org/10.1039/d1cp01985f
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