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A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome
BACKGROUND: Acute respiratory distress syndrome (ARDS) is multifactorial and can result from sepsis, trauma, or pneumonia, amongst other primary pathologies. It is one of the major causes of death in critically ill patients with a reported mortality rate up to 45%. The present study focuses on the d...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261975/ https://www.ncbi.nlm.nih.gov/pubmed/34233680 http://dx.doi.org/10.1186/s12931-021-01788-8 |
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author | Leiphrakpam, Premila D. Weber, Hannah R. McCain, Andrea Matas, Roser Romaguera Duarte, Ernesto Martinez Buesing, Keely L. |
author_facet | Leiphrakpam, Premila D. Weber, Hannah R. McCain, Andrea Matas, Roser Romaguera Duarte, Ernesto Martinez Buesing, Keely L. |
author_sort | Leiphrakpam, Premila D. |
collection | PubMed |
description | BACKGROUND: Acute respiratory distress syndrome (ARDS) is multifactorial and can result from sepsis, trauma, or pneumonia, amongst other primary pathologies. It is one of the major causes of death in critically ill patients with a reported mortality rate up to 45%. The present study focuses on the development of a large animal model of smoke inhalation-induced ARDS in an effort to provide the scientific community with a reliable, reproducible large animal model of isolated toxic inhalation injury-induced ARDS. METHODS: Animals (n = 21) were exposed to smoke under general anesthesia for 1 to 2 h (median smoke exposure = 0.5 to 1 L of oak wood smoke) after the ultrasound-guided placement of carotid, pulmonary, and femoral artery catheters. Peripheral oxygen saturation (SpO(2)), vital signs, and ventilator parameters were monitored throughout the procedure. Chest x-ray, carotid, femoral and pulmonary artery blood samples were collected before, during, and after smoke exposure. Animals were euthanized and lung tissue collected for analysis 48 h after smoke inhalation. RESULTS: Animals developed ARDS 48 h after smoke inhalation as reflected by a decrease in SpO(2) by approximately 31%, PaO(2)/FiO(2) ratio by approximately 208 (50%), and development of bilateral, diffuse infiltrates on chest x-ray. Study animals also demonstrated a significant increase in IL-6 level, lung tissue injury score and wet/dry ratio, as well as changes in other arterial blood gas (ABG) parameters. CONCLUSIONS: This study reports, for the first time, a novel large animal model of isolated smoke inhalation-induced ARDS without confounding variables such as cutaneous burn injury. Use of this unique model may be of benefit in studying the pathophysiology of inhalation injury or for development of novel therapeutics. |
format | Online Article Text |
id | pubmed-8261975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-82619752021-07-07 A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome Leiphrakpam, Premila D. Weber, Hannah R. McCain, Andrea Matas, Roser Romaguera Duarte, Ernesto Martinez Buesing, Keely L. Respir Res Research BACKGROUND: Acute respiratory distress syndrome (ARDS) is multifactorial and can result from sepsis, trauma, or pneumonia, amongst other primary pathologies. It is one of the major causes of death in critically ill patients with a reported mortality rate up to 45%. The present study focuses on the development of a large animal model of smoke inhalation-induced ARDS in an effort to provide the scientific community with a reliable, reproducible large animal model of isolated toxic inhalation injury-induced ARDS. METHODS: Animals (n = 21) were exposed to smoke under general anesthesia for 1 to 2 h (median smoke exposure = 0.5 to 1 L of oak wood smoke) after the ultrasound-guided placement of carotid, pulmonary, and femoral artery catheters. Peripheral oxygen saturation (SpO(2)), vital signs, and ventilator parameters were monitored throughout the procedure. Chest x-ray, carotid, femoral and pulmonary artery blood samples were collected before, during, and after smoke exposure. Animals were euthanized and lung tissue collected for analysis 48 h after smoke inhalation. RESULTS: Animals developed ARDS 48 h after smoke inhalation as reflected by a decrease in SpO(2) by approximately 31%, PaO(2)/FiO(2) ratio by approximately 208 (50%), and development of bilateral, diffuse infiltrates on chest x-ray. Study animals also demonstrated a significant increase in IL-6 level, lung tissue injury score and wet/dry ratio, as well as changes in other arterial blood gas (ABG) parameters. CONCLUSIONS: This study reports, for the first time, a novel large animal model of isolated smoke inhalation-induced ARDS without confounding variables such as cutaneous burn injury. Use of this unique model may be of benefit in studying the pathophysiology of inhalation injury or for development of novel therapeutics. BioMed Central 2021-07-07 2021 /pmc/articles/PMC8261975/ /pubmed/34233680 http://dx.doi.org/10.1186/s12931-021-01788-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Leiphrakpam, Premila D. Weber, Hannah R. McCain, Andrea Matas, Roser Romaguera Duarte, Ernesto Martinez Buesing, Keely L. A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome |
title | A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome |
title_full | A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome |
title_fullStr | A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome |
title_full_unstemmed | A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome |
title_short | A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome |
title_sort | novel large animal model of smoke inhalation-induced acute respiratory distress syndrome |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261975/ https://www.ncbi.nlm.nih.gov/pubmed/34233680 http://dx.doi.org/10.1186/s12931-021-01788-8 |
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