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Glycolate oxidase-dependent H(2)O(2) production regulates IAA biosynthesis in rice
BACKGROUND: Glycolate oxidase (GLO) is not only a key enzyme in photorespiration but also a major engine for H(2)O(2) production in plants. Catalase (CAT)-dependent H(2)O(2) decomposition has been previously reported to be involved in the regulation of IAA biosynthesis. However, it is still not know...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261990/ https://www.ncbi.nlm.nih.gov/pubmed/34229625 http://dx.doi.org/10.1186/s12870-021-03112-4 |
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author | Li, Xiangyang Liao, Mengmeng Huang, Jiayu Xu, Zheng Lin, Zhanqiao Ye, Nenghui Zhang, Zhisheng Peng, Xinxiang |
author_facet | Li, Xiangyang Liao, Mengmeng Huang, Jiayu Xu, Zheng Lin, Zhanqiao Ye, Nenghui Zhang, Zhisheng Peng, Xinxiang |
author_sort | Li, Xiangyang |
collection | PubMed |
description | BACKGROUND: Glycolate oxidase (GLO) is not only a key enzyme in photorespiration but also a major engine for H(2)O(2) production in plants. Catalase (CAT)-dependent H(2)O(2) decomposition has been previously reported to be involved in the regulation of IAA biosynthesis. However, it is still not known which mechanism contributed to the H(2)O(2) production in IAA regulation. RESULTS: In this study, we found that in glo mutants of rice, as H(2)O(2) levels decreased IAA contents significantly increased, whereas high CO(2) abolished the difference in H(2)O(2) and IAA contents between glo mutants and WT. Further analyses showed that tryptophan (Trp, the precursor for IAA biosynthesis in the Trp-dependent biosynthetic pathway) also accumulated due to increased tryptophan synthetase β (TSB) activity. Moreover, expression of the genes involved in Trp-dependent IAA biosynthesis and IBA to IAA conversion were correspondingly up-regulated, further implicating that both pathways contribute to IAA biosynthesis as mediated by the GLO-dependent production of H(2)O(2). CONCLUSION: We investigated the function of GLO in IAA signaling in different levels from transcription, enzyme activities to metabolic levels. The results suggest that GLO-dependent H(2)O(2) signaling, essentially via photorespiration, confers regulation over IAA biosynthesis in rice plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03112-4. |
format | Online Article Text |
id | pubmed-8261990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-82619902021-07-07 Glycolate oxidase-dependent H(2)O(2) production regulates IAA biosynthesis in rice Li, Xiangyang Liao, Mengmeng Huang, Jiayu Xu, Zheng Lin, Zhanqiao Ye, Nenghui Zhang, Zhisheng Peng, Xinxiang BMC Plant Biol Research Article BACKGROUND: Glycolate oxidase (GLO) is not only a key enzyme in photorespiration but also a major engine for H(2)O(2) production in plants. Catalase (CAT)-dependent H(2)O(2) decomposition has been previously reported to be involved in the regulation of IAA biosynthesis. However, it is still not known which mechanism contributed to the H(2)O(2) production in IAA regulation. RESULTS: In this study, we found that in glo mutants of rice, as H(2)O(2) levels decreased IAA contents significantly increased, whereas high CO(2) abolished the difference in H(2)O(2) and IAA contents between glo mutants and WT. Further analyses showed that tryptophan (Trp, the precursor for IAA biosynthesis in the Trp-dependent biosynthetic pathway) also accumulated due to increased tryptophan synthetase β (TSB) activity. Moreover, expression of the genes involved in Trp-dependent IAA biosynthesis and IBA to IAA conversion were correspondingly up-regulated, further implicating that both pathways contribute to IAA biosynthesis as mediated by the GLO-dependent production of H(2)O(2). CONCLUSION: We investigated the function of GLO in IAA signaling in different levels from transcription, enzyme activities to metabolic levels. The results suggest that GLO-dependent H(2)O(2) signaling, essentially via photorespiration, confers regulation over IAA biosynthesis in rice plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03112-4. BioMed Central 2021-07-06 /pmc/articles/PMC8261990/ /pubmed/34229625 http://dx.doi.org/10.1186/s12870-021-03112-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Li, Xiangyang Liao, Mengmeng Huang, Jiayu Xu, Zheng Lin, Zhanqiao Ye, Nenghui Zhang, Zhisheng Peng, Xinxiang Glycolate oxidase-dependent H(2)O(2) production regulates IAA biosynthesis in rice |
title | Glycolate oxidase-dependent H(2)O(2) production regulates IAA biosynthesis in rice |
title_full | Glycolate oxidase-dependent H(2)O(2) production regulates IAA biosynthesis in rice |
title_fullStr | Glycolate oxidase-dependent H(2)O(2) production regulates IAA biosynthesis in rice |
title_full_unstemmed | Glycolate oxidase-dependent H(2)O(2) production regulates IAA biosynthesis in rice |
title_short | Glycolate oxidase-dependent H(2)O(2) production regulates IAA biosynthesis in rice |
title_sort | glycolate oxidase-dependent h(2)o(2) production regulates iaa biosynthesis in rice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261990/ https://www.ncbi.nlm.nih.gov/pubmed/34229625 http://dx.doi.org/10.1186/s12870-021-03112-4 |
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