Cargando…

Napsin A Expression in Human Tumors and Normal Tissues

Background: Novel aspartic proteinase of the pepsin family A (Napsin A, TAO1/TAO2) is a functional aspartic proteinase which is involved in the maturation of prosurfactant protein B in type II pneumocytes and the lysosomal protein catabolism in renal cells. Napsin A is highly expressed in adenocarci...

Descripción completa

Detalles Bibliográficos
Autores principales: Weidemann, Sören, Böhle, Jan Lukas, Contreras, Hendrina, Luebke, Andreas M., Kluth, Martina, Büscheck, Franziska, Hube-Magg, Claudia, Höflmayer, Doris, Möller, Katharina, Fraune, Christoph, Bernreuther, Christian, Rink, Michael, Simon, Ronald, Menz, Anne, Hinsch, Andrea, Lebok, Patrick, Clauditz, Till, Sauter, Guido, Uhlig, Ria, Wilczak, Waldemar, Steurer, Stefan, Burandt, Eike, Krech, Rainer, Dum, David, Krech, Till, Marx, Andreas, Minner, Sarah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262149/
https://www.ncbi.nlm.nih.gov/pubmed/34257582
http://dx.doi.org/10.3389/pore.2021.613099
_version_ 1783719132925001728
author Weidemann, Sören
Böhle, Jan Lukas
Contreras, Hendrina
Luebke, Andreas M.
Kluth, Martina
Büscheck, Franziska
Hube-Magg, Claudia
Höflmayer, Doris
Möller, Katharina
Fraune, Christoph
Bernreuther, Christian
Rink, Michael
Simon, Ronald
Menz, Anne
Hinsch, Andrea
Lebok, Patrick
Clauditz, Till
Sauter, Guido
Uhlig, Ria
Wilczak, Waldemar
Steurer, Stefan
Burandt, Eike
Krech, Rainer
Dum, David
Krech, Till
Marx, Andreas
Minner, Sarah
author_facet Weidemann, Sören
Böhle, Jan Lukas
Contreras, Hendrina
Luebke, Andreas M.
Kluth, Martina
Büscheck, Franziska
Hube-Magg, Claudia
Höflmayer, Doris
Möller, Katharina
Fraune, Christoph
Bernreuther, Christian
Rink, Michael
Simon, Ronald
Menz, Anne
Hinsch, Andrea
Lebok, Patrick
Clauditz, Till
Sauter, Guido
Uhlig, Ria
Wilczak, Waldemar
Steurer, Stefan
Burandt, Eike
Krech, Rainer
Dum, David
Krech, Till
Marx, Andreas
Minner, Sarah
author_sort Weidemann, Sören
collection PubMed
description Background: Novel aspartic proteinase of the pepsin family A (Napsin A, TAO1/TAO2) is a functional aspartic proteinase which is involved in the maturation of prosurfactant protein B in type II pneumocytes and the lysosomal protein catabolism in renal cells. Napsin A is highly expressed in adenocarcinomas of the lung and is thus commonly used to affirm this diagnosis. However, studies have shown that other tumors can also express Napsin A. Methods: To comprehensively determine Napsin A expression in normal and tumor tissue, 11,957 samples from 115 different tumor types and subtypes as well as 500 samples of 76 different normal tissue types were evaluable by immunohistochemistry on tissue microarrays. Results: Napsin A expression was present in 16 different tumor types. Adenocarcinoma of the lung (85.6%), clear cell adenocarcinoma of the ovary (71.7%), clear cell adenocarcinoma of the endometrium (42.8%), papillary renal cell carcinoma (40.2%), clear cell (tubulo) papillary renal cell carcinoma (16.7%), endometrial serous carcinoma (9.3%), papillary thyroid carcinoma (9.3%) and clear cell renal cell carcinoma (8.2%) were among the tumors with the highest prevalence of Napsin A positivity. In papillary and clear cell renal cell carcinoma, reduced Napsin A expression was linked to adverse clinic-pathological features (p ≤ 0.03). Conclusion: This methodical approach enabled us to identify a ranking order of tumors according to their relative prevalence of Napsin A expression. The data also show that loss of Napsin A is linked to tumor dedifferentiation in renal cell carcinomas.
format Online
Article
Text
id pubmed-8262149
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82621492021-07-12 Napsin A Expression in Human Tumors and Normal Tissues Weidemann, Sören Böhle, Jan Lukas Contreras, Hendrina Luebke, Andreas M. Kluth, Martina Büscheck, Franziska Hube-Magg, Claudia Höflmayer, Doris Möller, Katharina Fraune, Christoph Bernreuther, Christian Rink, Michael Simon, Ronald Menz, Anne Hinsch, Andrea Lebok, Patrick Clauditz, Till Sauter, Guido Uhlig, Ria Wilczak, Waldemar Steurer, Stefan Burandt, Eike Krech, Rainer Dum, David Krech, Till Marx, Andreas Minner, Sarah Pathol Oncol Res Society Journal Archive Background: Novel aspartic proteinase of the pepsin family A (Napsin A, TAO1/TAO2) is a functional aspartic proteinase which is involved in the maturation of prosurfactant protein B in type II pneumocytes and the lysosomal protein catabolism in renal cells. Napsin A is highly expressed in adenocarcinomas of the lung and is thus commonly used to affirm this diagnosis. However, studies have shown that other tumors can also express Napsin A. Methods: To comprehensively determine Napsin A expression in normal and tumor tissue, 11,957 samples from 115 different tumor types and subtypes as well as 500 samples of 76 different normal tissue types were evaluable by immunohistochemistry on tissue microarrays. Results: Napsin A expression was present in 16 different tumor types. Adenocarcinoma of the lung (85.6%), clear cell adenocarcinoma of the ovary (71.7%), clear cell adenocarcinoma of the endometrium (42.8%), papillary renal cell carcinoma (40.2%), clear cell (tubulo) papillary renal cell carcinoma (16.7%), endometrial serous carcinoma (9.3%), papillary thyroid carcinoma (9.3%) and clear cell renal cell carcinoma (8.2%) were among the tumors with the highest prevalence of Napsin A positivity. In papillary and clear cell renal cell carcinoma, reduced Napsin A expression was linked to adverse clinic-pathological features (p ≤ 0.03). Conclusion: This methodical approach enabled us to identify a ranking order of tumors according to their relative prevalence of Napsin A expression. The data also show that loss of Napsin A is linked to tumor dedifferentiation in renal cell carcinomas. Frontiers Media S.A. 2021-04-20 /pmc/articles/PMC8262149/ /pubmed/34257582 http://dx.doi.org/10.3389/pore.2021.613099 Text en Copyright © 2021 Weidemann, Böhle, Contreras, Luebke, Kluth, Büscheck, Hube-Magg, Höflmayer, Möller, Fraune, Bernreuther, Rink, Simon, Menz, Hinsch, Lebok, Clauditz, Sauter, Uhlig, Wilczak, Steurer, Burandt, Krech, Dum, Krech, Marx and Minner. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Society Journal Archive
Weidemann, Sören
Böhle, Jan Lukas
Contreras, Hendrina
Luebke, Andreas M.
Kluth, Martina
Büscheck, Franziska
Hube-Magg, Claudia
Höflmayer, Doris
Möller, Katharina
Fraune, Christoph
Bernreuther, Christian
Rink, Michael
Simon, Ronald
Menz, Anne
Hinsch, Andrea
Lebok, Patrick
Clauditz, Till
Sauter, Guido
Uhlig, Ria
Wilczak, Waldemar
Steurer, Stefan
Burandt, Eike
Krech, Rainer
Dum, David
Krech, Till
Marx, Andreas
Minner, Sarah
Napsin A Expression in Human Tumors and Normal Tissues
title Napsin A Expression in Human Tumors and Normal Tissues
title_full Napsin A Expression in Human Tumors and Normal Tissues
title_fullStr Napsin A Expression in Human Tumors and Normal Tissues
title_full_unstemmed Napsin A Expression in Human Tumors and Normal Tissues
title_short Napsin A Expression in Human Tumors and Normal Tissues
title_sort napsin a expression in human tumors and normal tissues
topic Society Journal Archive
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262149/
https://www.ncbi.nlm.nih.gov/pubmed/34257582
http://dx.doi.org/10.3389/pore.2021.613099
work_keys_str_mv AT weidemannsoren napsinaexpressioninhumantumorsandnormaltissues
AT bohlejanlukas napsinaexpressioninhumantumorsandnormaltissues
AT contrerashendrina napsinaexpressioninhumantumorsandnormaltissues
AT luebkeandreasm napsinaexpressioninhumantumorsandnormaltissues
AT kluthmartina napsinaexpressioninhumantumorsandnormaltissues
AT buscheckfranziska napsinaexpressioninhumantumorsandnormaltissues
AT hubemaggclaudia napsinaexpressioninhumantumorsandnormaltissues
AT hoflmayerdoris napsinaexpressioninhumantumorsandnormaltissues
AT mollerkatharina napsinaexpressioninhumantumorsandnormaltissues
AT fraunechristoph napsinaexpressioninhumantumorsandnormaltissues
AT bernreutherchristian napsinaexpressioninhumantumorsandnormaltissues
AT rinkmichael napsinaexpressioninhumantumorsandnormaltissues
AT simonronald napsinaexpressioninhumantumorsandnormaltissues
AT menzanne napsinaexpressioninhumantumorsandnormaltissues
AT hinschandrea napsinaexpressioninhumantumorsandnormaltissues
AT lebokpatrick napsinaexpressioninhumantumorsandnormaltissues
AT clauditztill napsinaexpressioninhumantumorsandnormaltissues
AT sauterguido napsinaexpressioninhumantumorsandnormaltissues
AT uhligria napsinaexpressioninhumantumorsandnormaltissues
AT wilczakwaldemar napsinaexpressioninhumantumorsandnormaltissues
AT steurerstefan napsinaexpressioninhumantumorsandnormaltissues
AT burandteike napsinaexpressioninhumantumorsandnormaltissues
AT krechrainer napsinaexpressioninhumantumorsandnormaltissues
AT dumdavid napsinaexpressioninhumantumorsandnormaltissues
AT krechtill napsinaexpressioninhumantumorsandnormaltissues
AT marxandreas napsinaexpressioninhumantumorsandnormaltissues
AT minnersarah napsinaexpressioninhumantumorsandnormaltissues