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Single Cell Gene Transcriptome Analysis of Ovarian Mature Teratomas
Teratoma is a type of germ cell tumor that originates from totipotential germ cells that are present in gonads, which can differentiate into any of the cell types found in adult tissues. Ovarian teratomas are usually mature cystic teratomas (OMCTs, also known as dermoid cysts). Chromosome studies in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262198/ https://www.ncbi.nlm.nih.gov/pubmed/34257564 http://dx.doi.org/10.3389/pore.2021.604228 |
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author | Shin, Sun Choi, Youn Jin Jung, Seung-Hyun Chung, Yeun-Jun Lee, Sug Hyung |
author_facet | Shin, Sun Choi, Youn Jin Jung, Seung-Hyun Chung, Yeun-Jun Lee, Sug Hyung |
author_sort | Shin, Sun |
collection | PubMed |
description | Teratoma is a type of germ cell tumor that originates from totipotential germ cells that are present in gonads, which can differentiate into any of the cell types found in adult tissues. Ovarian teratomas are usually mature cystic teratomas (OMCTs, also known as dermoid cysts). Chromosome studies in OMCTs show that the chromosomes are uniformly homozygous with karyotype of 46, XX, indicating that they may be parthenogenic tumors that arise from a single ovum after thefirst meiotic division. However, the tissues in OMCTs have been known to be morphologically and immunophenotypically identical to the orthotopic tissues. Currently, expression profiles of tissue components in OMCTs are not known. To identify whether OMCT tissues are expressionally similar to or different from the orthotopic tissues, we adopted single-cell RNA-sequencing (scRNA-seq), and analyzed transcriptomes of individual cells in heterogenous tissues of two OMCTs. We found that transcriptome profiles of the OMCTs at single cell level were not significantly different from those of normal cells in orthotopic locations. The present data suggest that parthenogeneticlly altered OMCTs may not alter expression profiles of inrivirual tissue components in OMCTs. |
format | Online Article Text |
id | pubmed-8262198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82621982021-07-12 Single Cell Gene Transcriptome Analysis of Ovarian Mature Teratomas Shin, Sun Choi, Youn Jin Jung, Seung-Hyun Chung, Yeun-Jun Lee, Sug Hyung Pathol Oncol Res Society Journal Archive Teratoma is a type of germ cell tumor that originates from totipotential germ cells that are present in gonads, which can differentiate into any of the cell types found in adult tissues. Ovarian teratomas are usually mature cystic teratomas (OMCTs, also known as dermoid cysts). Chromosome studies in OMCTs show that the chromosomes are uniformly homozygous with karyotype of 46, XX, indicating that they may be parthenogenic tumors that arise from a single ovum after thefirst meiotic division. However, the tissues in OMCTs have been known to be morphologically and immunophenotypically identical to the orthotopic tissues. Currently, expression profiles of tissue components in OMCTs are not known. To identify whether OMCT tissues are expressionally similar to or different from the orthotopic tissues, we adopted single-cell RNA-sequencing (scRNA-seq), and analyzed transcriptomes of individual cells in heterogenous tissues of two OMCTs. We found that transcriptome profiles of the OMCTs at single cell level were not significantly different from those of normal cells in orthotopic locations. The present data suggest that parthenogeneticlly altered OMCTs may not alter expression profiles of inrivirual tissue components in OMCTs. Frontiers Media S.A. 2021-04-16 /pmc/articles/PMC8262198/ /pubmed/34257564 http://dx.doi.org/10.3389/pore.2021.604228 Text en Copyright © 2021 Shin, Choi, Jung, Chung and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Society Journal Archive Shin, Sun Choi, Youn Jin Jung, Seung-Hyun Chung, Yeun-Jun Lee, Sug Hyung Single Cell Gene Transcriptome Analysis of Ovarian Mature Teratomas |
title | Single Cell Gene Transcriptome Analysis of Ovarian Mature Teratomas |
title_full | Single Cell Gene Transcriptome Analysis of Ovarian Mature Teratomas |
title_fullStr | Single Cell Gene Transcriptome Analysis of Ovarian Mature Teratomas |
title_full_unstemmed | Single Cell Gene Transcriptome Analysis of Ovarian Mature Teratomas |
title_short | Single Cell Gene Transcriptome Analysis of Ovarian Mature Teratomas |
title_sort | single cell gene transcriptome analysis of ovarian mature teratomas |
topic | Society Journal Archive |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262198/ https://www.ncbi.nlm.nih.gov/pubmed/34257564 http://dx.doi.org/10.3389/pore.2021.604228 |
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