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Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers

TRAF2 and TRAF3 genes of tumor necrosis factor receptor (TNF-R)-associated factor (TRAF) family are involved in diverse cell signaling, and function as both tumor suppressor gene and oncogene. Alterations of TRAF2 and TRAF3 in colon cancer (CC) along with their regional difference and microsatellite...

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Autores principales: Moon, Seong Won, Son, Hyun Ji, Choi, Eun Ji, Yoo, Nam Jin, Lee, Sug Hyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262244/
https://www.ncbi.nlm.nih.gov/pubmed/34257589
http://dx.doi.org/10.3389/pore.2021.625438
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author Moon, Seong Won
Son, Hyun Ji
Choi, Eun Ji
Yoo, Nam Jin
Lee, Sug Hyung
author_facet Moon, Seong Won
Son, Hyun Ji
Choi, Eun Ji
Yoo, Nam Jin
Lee, Sug Hyung
author_sort Moon, Seong Won
collection PubMed
description TRAF2 and TRAF3 genes of tumor necrosis factor receptor (TNF-R)-associated factor (TRAF) family are involved in diverse cell signaling, and function as both tumor suppressor gene and oncogene. Alterations of TRAF2 and TRAF3 in colon cancer (CC) along with their regional difference and microsatellite instability (MSI) are largely unknown. In the present study, we analyzed TRAF2 and TRAF3 frameshift mutations in 168 sporadic CCs (100 high MSI (MSI-H) and 68 microsatellite-stable (MSS) CCs). We identified TRAF2 and TRAF3 frameshift mutations in 4 (4%) and 3 CCs (3%) with MSI-H, respectively, but none in 68 cases of MSS CCs. Of the 168 CCs, we analyzed the mutations in multi-regions for 39 CCs (16 MSI-H and 23 MSS CCs), and discovered that 12.5% (2/16) and 6.3% (1/16) of MSI-H CCs exhibited regional difference in TRAF2 and TRAF3 mutations, respectively. In the multi-region samples of 23 MSS CCs, neither TRAF2 nor TRAF3 frameshift mutation was found. In 40% of CCs, both TRAF2 and TRAF3 expressions were increased compared to normal colon cells. Our data indicate that TRAF2 and TRAF3 frameshift mutations and their regional difference as well as altered expressions are present in MSI-H CCs, which could contribute to MSI-H cancer development.
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spelling pubmed-82622442021-07-12 Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers Moon, Seong Won Son, Hyun Ji Choi, Eun Ji Yoo, Nam Jin Lee, Sug Hyung Pathol Oncol Res Society Journal Archive TRAF2 and TRAF3 genes of tumor necrosis factor receptor (TNF-R)-associated factor (TRAF) family are involved in diverse cell signaling, and function as both tumor suppressor gene and oncogene. Alterations of TRAF2 and TRAF3 in colon cancer (CC) along with their regional difference and microsatellite instability (MSI) are largely unknown. In the present study, we analyzed TRAF2 and TRAF3 frameshift mutations in 168 sporadic CCs (100 high MSI (MSI-H) and 68 microsatellite-stable (MSS) CCs). We identified TRAF2 and TRAF3 frameshift mutations in 4 (4%) and 3 CCs (3%) with MSI-H, respectively, but none in 68 cases of MSS CCs. Of the 168 CCs, we analyzed the mutations in multi-regions for 39 CCs (16 MSI-H and 23 MSS CCs), and discovered that 12.5% (2/16) and 6.3% (1/16) of MSI-H CCs exhibited regional difference in TRAF2 and TRAF3 mutations, respectively. In the multi-region samples of 23 MSS CCs, neither TRAF2 nor TRAF3 frameshift mutation was found. In 40% of CCs, both TRAF2 and TRAF3 expressions were increased compared to normal colon cells. Our data indicate that TRAF2 and TRAF3 frameshift mutations and their regional difference as well as altered expressions are present in MSI-H CCs, which could contribute to MSI-H cancer development. Frontiers Media S.A. 2021-04-14 /pmc/articles/PMC8262244/ /pubmed/34257589 http://dx.doi.org/10.3389/pore.2021.625438 Text en Copyright © 2021 Moon, Son, Choi, Yoo and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Society Journal Archive
Moon, Seong Won
Son, Hyun Ji
Choi, Eun Ji
Yoo, Nam Jin
Lee, Sug Hyung
Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers
title Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers
title_full Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers
title_fullStr Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers
title_full_unstemmed Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers
title_short Brief Research Report Regional Difference in TRAF2 and TRAF3 Gene Mutations in Colon Cancers
title_sort brief research report regional difference in traf2 and traf3 gene mutations in colon cancers
topic Society Journal Archive
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262244/
https://www.ncbi.nlm.nih.gov/pubmed/34257589
http://dx.doi.org/10.3389/pore.2021.625438
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