Cargando…
Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus
Emerging coronaviruses from zoonotic reservoirs, including severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have been associated with human-to-human transmission and si...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Clinical Investigation
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262283/ https://www.ncbi.nlm.nih.gov/pubmed/33886507 http://dx.doi.org/10.1172/jci.insight.146082 |
_version_ | 1783719161077170176 |
---|---|
author | Patel, Ami Reuschel, Emma L. Xu, Ziyang Zaidi, Faraz I. Kim, Kevin Y. Scott, Dana P. Mendoza, Janess Ramos, Stephanie Stoltz, Regina Feldmann, Friederike Okumura, Atsushi Meade-White, Kimberly Haddock, Elaine Thomas, Tina Rosenke, Rebecca Lovaglio, Jamie Hanley, Patrick W. Saturday, Greg Muthumani, Kar Feldmann, Heinz Humeau, Laurent M. Broderick, Kate E. Weiner, David B. |
author_facet | Patel, Ami Reuschel, Emma L. Xu, Ziyang Zaidi, Faraz I. Kim, Kevin Y. Scott, Dana P. Mendoza, Janess Ramos, Stephanie Stoltz, Regina Feldmann, Friederike Okumura, Atsushi Meade-White, Kimberly Haddock, Elaine Thomas, Tina Rosenke, Rebecca Lovaglio, Jamie Hanley, Patrick W. Saturday, Greg Muthumani, Kar Feldmann, Heinz Humeau, Laurent M. Broderick, Kate E. Weiner, David B. |
author_sort | Patel, Ami |
collection | PubMed |
description | Emerging coronaviruses from zoonotic reservoirs, including severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have been associated with human-to-human transmission and significant morbidity and mortality. Here, we study both intradermal and intramuscular 2-dose delivery regimens of an advanced synthetic DNA vaccine candidate encoding a full-length MERS-CoV spike (S) protein, which induced potent binding and neutralizing antibodies as well as cellular immune responses in rhesus macaques. In a MERS-CoV challenge, all immunized rhesus macaques exhibited reduced clinical symptoms, lowered viral lung load, and decreased severity of pathological signs of disease compared with controls. Intradermal vaccination was dose sparing and more effective in this model at protecting animals from disease. The data support the further study of this vaccine for preventing MERS-CoV infection and transmission, including investigation of such vaccines and simplified delivery routes against emerging coronaviruses. |
format | Online Article Text |
id | pubmed-8262283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Clinical Investigation |
record_format | MEDLINE/PubMed |
spelling | pubmed-82622832021-07-13 Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus Patel, Ami Reuschel, Emma L. Xu, Ziyang Zaidi, Faraz I. Kim, Kevin Y. Scott, Dana P. Mendoza, Janess Ramos, Stephanie Stoltz, Regina Feldmann, Friederike Okumura, Atsushi Meade-White, Kimberly Haddock, Elaine Thomas, Tina Rosenke, Rebecca Lovaglio, Jamie Hanley, Patrick W. Saturday, Greg Muthumani, Kar Feldmann, Heinz Humeau, Laurent M. Broderick, Kate E. Weiner, David B. JCI Insight Research Article Emerging coronaviruses from zoonotic reservoirs, including severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have been associated with human-to-human transmission and significant morbidity and mortality. Here, we study both intradermal and intramuscular 2-dose delivery regimens of an advanced synthetic DNA vaccine candidate encoding a full-length MERS-CoV spike (S) protein, which induced potent binding and neutralizing antibodies as well as cellular immune responses in rhesus macaques. In a MERS-CoV challenge, all immunized rhesus macaques exhibited reduced clinical symptoms, lowered viral lung load, and decreased severity of pathological signs of disease compared with controls. Intradermal vaccination was dose sparing and more effective in this model at protecting animals from disease. The data support the further study of this vaccine for preventing MERS-CoV infection and transmission, including investigation of such vaccines and simplified delivery routes against emerging coronaviruses. American Society for Clinical Investigation 2021-05-24 /pmc/articles/PMC8262283/ /pubmed/33886507 http://dx.doi.org/10.1172/jci.insight.146082 Text en © 2021 Patel et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Patel, Ami Reuschel, Emma L. Xu, Ziyang Zaidi, Faraz I. Kim, Kevin Y. Scott, Dana P. Mendoza, Janess Ramos, Stephanie Stoltz, Regina Feldmann, Friederike Okumura, Atsushi Meade-White, Kimberly Haddock, Elaine Thomas, Tina Rosenke, Rebecca Lovaglio, Jamie Hanley, Patrick W. Saturday, Greg Muthumani, Kar Feldmann, Heinz Humeau, Laurent M. Broderick, Kate E. Weiner, David B. Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus |
title | Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus |
title_full | Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus |
title_fullStr | Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus |
title_full_unstemmed | Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus |
title_short | Intradermal delivery of a synthetic DNA vaccine protects macaques from Middle East respiratory syndrome coronavirus |
title_sort | intradermal delivery of a synthetic dna vaccine protects macaques from middle east respiratory syndrome coronavirus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262283/ https://www.ncbi.nlm.nih.gov/pubmed/33886507 http://dx.doi.org/10.1172/jci.insight.146082 |
work_keys_str_mv | AT patelami intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT reuschelemmal intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT xuziyang intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT zaidifarazi intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT kimkeviny intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT scottdanap intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT mendozajaness intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT ramosstephanie intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT stoltzregina intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT feldmannfriederike intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT okumuraatsushi intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT meadewhitekimberly intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT haddockelaine intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT thomastina intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT rosenkerebecca intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT lovagliojamie intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT hanleypatrickw intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT saturdaygreg intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT muthumanikar intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT feldmannheinz intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT humeaulaurentm intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT broderickkatee intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus AT weinerdavidb intradermaldeliveryofasyntheticdnavaccineprotectsmacaquesfrommiddleeastrespiratorysyndromecoronavirus |