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Preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions

Preselection digests are a common strategy to reduce the background in the ligation step of molecular cloning. However, choosing fitting restriction enzymes by hand is not trivial and may lead to errors, potentially costing a lot of time and work. We therefore created preselector.uni-jena.de (https:...

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Detalles Bibliográficos
Autores principales: Gühmann, Martin, Reuter, Stefanie, Hartung, Jan, Mrowka, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262699/
https://www.ncbi.nlm.nih.gov/pubmed/34037777
http://dx.doi.org/10.1093/nar/gkab406
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author Gühmann, Martin
Reuter, Stefanie
Hartung, Jan
Mrowka, Ralf
author_facet Gühmann, Martin
Reuter, Stefanie
Hartung, Jan
Mrowka, Ralf
author_sort Gühmann, Martin
collection PubMed
description Preselection digests are a common strategy to reduce the background in the ligation step of molecular cloning. However, choosing fitting restriction enzymes by hand is not trivial and may lead to errors, potentially costing a lot of time and work. We therefore created preselector.uni-jena.de (https://preselector.uni-jena.de/), a free online tool to find such restriction enzymes. The tool uses regular expressions to find recognition sites of restriction enzymes in the DNA sequences provided by the user. This new tool compares the sets of restriction sites and reports the enzymes that cut one sequence but not the other sequences to the user. These enzymes are then the ones suitable for a preselection digest. Thus, preselector.uni-jena.de is a fast, reliable, and free-to-use tool to help researchers designing preselection digestion strategies for their cloning.
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spelling pubmed-82626992021-07-08 Preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions Gühmann, Martin Reuter, Stefanie Hartung, Jan Mrowka, Ralf Nucleic Acids Res Web Server Issue Preselection digests are a common strategy to reduce the background in the ligation step of molecular cloning. However, choosing fitting restriction enzymes by hand is not trivial and may lead to errors, potentially costing a lot of time and work. We therefore created preselector.uni-jena.de (https://preselector.uni-jena.de/), a free online tool to find such restriction enzymes. The tool uses regular expressions to find recognition sites of restriction enzymes in the DNA sequences provided by the user. This new tool compares the sets of restriction sites and reports the enzymes that cut one sequence but not the other sequences to the user. These enzymes are then the ones suitable for a preselection digest. Thus, preselector.uni-jena.de is a fast, reliable, and free-to-use tool to help researchers designing preselection digestion strategies for their cloning. Oxford University Press 2021-05-25 /pmc/articles/PMC8262699/ /pubmed/34037777 http://dx.doi.org/10.1093/nar/gkab406 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Web Server Issue
Gühmann, Martin
Reuter, Stefanie
Hartung, Jan
Mrowka, Ralf
Preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions
title Preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions
title_full Preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions
title_fullStr Preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions
title_full_unstemmed Preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions
title_short Preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions
title_sort preselector.uni-jena.de: optimize your cloning—a resource for identifying restriction enzymes for preselection reactions
topic Web Server Issue
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262699/
https://www.ncbi.nlm.nih.gov/pubmed/34037777
http://dx.doi.org/10.1093/nar/gkab406
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