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De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media
Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found suc...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262816/ https://www.ncbi.nlm.nih.gov/pubmed/34233883 http://dx.doi.org/10.1126/sciadv.aay5496 |
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author | Zheng, Cheng Park, Jong Kang Yildirim, Murat Boivin, Josiah R. Xue, Yi Sur, Mriganka So, Peter T. C. Wadduwage, Dushan N. |
author_facet | Zheng, Cheng Park, Jong Kang Yildirim, Murat Boivin, Josiah R. Xue, Yi Sur, Mriganka So, Peter T. C. Wadduwage, Dushan N. |
author_sort | Zheng, Cheng |
collection | PubMed |
description | Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found successes in optically cleared tissues and at shallower depths, but the scattering of emission photons in thick turbid samples severely degrades image quality at the camera. To address this challenge, we introduce a novel technique called De-scattering with Excitation Patterning or “DEEP,” which uses patterned nonlinear excitation followed by computational imaging–assisted wide-field detection. Multiphoton temporal focusing allows high-resolution excitation patterns to be projected deep inside specimen at multiple scattering lengths due to the use of long wavelength light. Computational reconstruction allows high-resolution structural features to be reconstructed from tens to hundreds of DEEP images instead of millions of point-scanning measurements. |
format | Online Article Text |
id | pubmed-8262816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82628162021-07-16 De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media Zheng, Cheng Park, Jong Kang Yildirim, Murat Boivin, Josiah R. Xue, Yi Sur, Mriganka So, Peter T. C. Wadduwage, Dushan N. Sci Adv Research Articles Nonlinear optical microscopy has enabled in vivo deep tissue imaging on the millimeter scale. A key unmet challenge is its limited throughput especially compared to rapid wide-field modalities that are used ubiquitously in thin specimens. Wide-field imaging methods in tissue specimens have found successes in optically cleared tissues and at shallower depths, but the scattering of emission photons in thick turbid samples severely degrades image quality at the camera. To address this challenge, we introduce a novel technique called De-scattering with Excitation Patterning or “DEEP,” which uses patterned nonlinear excitation followed by computational imaging–assisted wide-field detection. Multiphoton temporal focusing allows high-resolution excitation patterns to be projected deep inside specimen at multiple scattering lengths due to the use of long wavelength light. Computational reconstruction allows high-resolution structural features to be reconstructed from tens to hundreds of DEEP images instead of millions of point-scanning measurements. American Association for the Advancement of Science 2021-07-07 /pmc/articles/PMC8262816/ /pubmed/34233883 http://dx.doi.org/10.1126/sciadv.aay5496 Text en Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Zheng, Cheng Park, Jong Kang Yildirim, Murat Boivin, Josiah R. Xue, Yi Sur, Mriganka So, Peter T. C. Wadduwage, Dushan N. De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
title | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
title_full | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
title_fullStr | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
title_full_unstemmed | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
title_short | De-scattering with Excitation Patterning enables rapid wide-field imaging through scattering media |
title_sort | de-scattering with excitation patterning enables rapid wide-field imaging through scattering media |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262816/ https://www.ncbi.nlm.nih.gov/pubmed/34233883 http://dx.doi.org/10.1126/sciadv.aay5496 |
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