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Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species

The thermotolerant multidrug-resistant ascomycete Candida auris rapidly emerged since 2009 causing systemic infections worldwide and simultaneously evolved in different geographical zones. The molecular events that orchestrated this sudden emergence of the killer fungus remain mostly elusive. Here,...

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Autores principales: Narayanan, Aswathy, Vadnala, Rakesh Netha, Ganguly, Promit, Selvakumar, Pavitra, Rudramurthy, Shivaprakash M., Prasad, Rajendra, Chakrabarti, Arunaloke, Siddharthan, Rahul, Sanyal, Kaustuv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262905/
https://www.ncbi.nlm.nih.gov/pubmed/33975937
http://dx.doi.org/10.1128/mBio.00905-21
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author Narayanan, Aswathy
Vadnala, Rakesh Netha
Ganguly, Promit
Selvakumar, Pavitra
Rudramurthy, Shivaprakash M.
Prasad, Rajendra
Chakrabarti, Arunaloke
Siddharthan, Rahul
Sanyal, Kaustuv
author_facet Narayanan, Aswathy
Vadnala, Rakesh Netha
Ganguly, Promit
Selvakumar, Pavitra
Rudramurthy, Shivaprakash M.
Prasad, Rajendra
Chakrabarti, Arunaloke
Siddharthan, Rahul
Sanyal, Kaustuv
author_sort Narayanan, Aswathy
collection PubMed
description The thermotolerant multidrug-resistant ascomycete Candida auris rapidly emerged since 2009 causing systemic infections worldwide and simultaneously evolved in different geographical zones. The molecular events that orchestrated this sudden emergence of the killer fungus remain mostly elusive. Here, we identify centromeres in C. auris and related species, using a combined approach of chromatin immunoprecipitation and comparative genomic analyses. We find that C. auris and multiple other species in the Clavispora/Candida clade shared a conserved small regional GC-poor centromere landscape lacking pericentromeres or repeats. Further, a centromere inactivation event led to karyotypic alterations in this species complex. Interspecies genome analysis identified several structural chromosomal changes around centromeres. In addition, centromeres are found to be rapidly evolving loci among the different geographical clades of the same species of C. auris. Finally, we reveal an evolutionary trajectory of the unique karyotype associated with clade 2 that consists of the drug-susceptible isolates of C. auris.
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spelling pubmed-82629052021-07-23 Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species Narayanan, Aswathy Vadnala, Rakesh Netha Ganguly, Promit Selvakumar, Pavitra Rudramurthy, Shivaprakash M. Prasad, Rajendra Chakrabarti, Arunaloke Siddharthan, Rahul Sanyal, Kaustuv mBio Research Article The thermotolerant multidrug-resistant ascomycete Candida auris rapidly emerged since 2009 causing systemic infections worldwide and simultaneously evolved in different geographical zones. The molecular events that orchestrated this sudden emergence of the killer fungus remain mostly elusive. Here, we identify centromeres in C. auris and related species, using a combined approach of chromatin immunoprecipitation and comparative genomic analyses. We find that C. auris and multiple other species in the Clavispora/Candida clade shared a conserved small regional GC-poor centromere landscape lacking pericentromeres or repeats. Further, a centromere inactivation event led to karyotypic alterations in this species complex. Interspecies genome analysis identified several structural chromosomal changes around centromeres. In addition, centromeres are found to be rapidly evolving loci among the different geographical clades of the same species of C. auris. Finally, we reveal an evolutionary trajectory of the unique karyotype associated with clade 2 that consists of the drug-susceptible isolates of C. auris. American Society for Microbiology 2021-05-11 /pmc/articles/PMC8262905/ /pubmed/33975937 http://dx.doi.org/10.1128/mBio.00905-21 Text en Copyright © 2021 Narayanan et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Narayanan, Aswathy
Vadnala, Rakesh Netha
Ganguly, Promit
Selvakumar, Pavitra
Rudramurthy, Shivaprakash M.
Prasad, Rajendra
Chakrabarti, Arunaloke
Siddharthan, Rahul
Sanyal, Kaustuv
Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species
title Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species
title_full Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species
title_fullStr Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species
title_full_unstemmed Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species
title_short Functional and Comparative Analysis of Centromeres Reveals Clade-Specific Genome Rearrangements in Candida auris and a Chromosome Number Change in Related Species
title_sort functional and comparative analysis of centromeres reveals clade-specific genome rearrangements in candida auris and a chromosome number change in related species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262905/
https://www.ncbi.nlm.nih.gov/pubmed/33975937
http://dx.doi.org/10.1128/mBio.00905-21
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