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Bacterial Metal Resistance: Coping with Copper without Cooperativity?
In Escherichia coli and other Gram-negative bacteria, tripartite efflux pumps (TEPs) span the entire cell envelope and serve to remove noxious molecules from the cell. CusBCA is a TEP responsible for copper and silver detoxification in E. coli powered by the resistance-nodulation-cell division (RND)...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Society for Microbiology
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262934/ https://www.ncbi.nlm.nih.gov/pubmed/34126768 http://dx.doi.org/10.1128/mBio.00653-21 |
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author | Greene, Nicholas P. Koronakis, Vassilis |
author_facet | Greene, Nicholas P. Koronakis, Vassilis |
author_sort | Greene, Nicholas P. |
collection | PubMed |
description | In Escherichia coli and other Gram-negative bacteria, tripartite efflux pumps (TEPs) span the entire cell envelope and serve to remove noxious molecules from the cell. CusBCA is a TEP responsible for copper and silver detoxification in E. coli powered by the resistance-nodulation-cell division (RND) transporter, CusA. In a recent study, Moseng et al. (M. A. Moseng, M. Lyu, T. Pipatpolkai, P. Glaza, et al., mBio 12:e00452-21, 2021, https://dx.doi.org/10.1128/mBio.00452-21) obtained cryo-electron microscopy (cryo-EM) structures of CusA trimers in the presence of copper. The multiple conformations revealed suggest that the three monomers function independently within the CusA trimer, contrary to the cooperative mechanism proposed for the multidrug exporting RND transporter, AcrB. The work prompts consideration of the mechanism of this class of transporter and provides a basis to underpin further studies of TEPs important for bacterial survival. |
format | Online Article Text |
id | pubmed-8262934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-82629342021-07-23 Bacterial Metal Resistance: Coping with Copper without Cooperativity? Greene, Nicholas P. Koronakis, Vassilis mBio Commentary In Escherichia coli and other Gram-negative bacteria, tripartite efflux pumps (TEPs) span the entire cell envelope and serve to remove noxious molecules from the cell. CusBCA is a TEP responsible for copper and silver detoxification in E. coli powered by the resistance-nodulation-cell division (RND) transporter, CusA. In a recent study, Moseng et al. (M. A. Moseng, M. Lyu, T. Pipatpolkai, P. Glaza, et al., mBio 12:e00452-21, 2021, https://dx.doi.org/10.1128/mBio.00452-21) obtained cryo-electron microscopy (cryo-EM) structures of CusA trimers in the presence of copper. The multiple conformations revealed suggest that the three monomers function independently within the CusA trimer, contrary to the cooperative mechanism proposed for the multidrug exporting RND transporter, AcrB. The work prompts consideration of the mechanism of this class of transporter and provides a basis to underpin further studies of TEPs important for bacterial survival. American Society for Microbiology 2021-06-15 /pmc/articles/PMC8262934/ /pubmed/34126768 http://dx.doi.org/10.1128/mBio.00653-21 Text en Copyright © 2021 Greene and Koronakis. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Commentary Greene, Nicholas P. Koronakis, Vassilis Bacterial Metal Resistance: Coping with Copper without Cooperativity? |
title | Bacterial Metal Resistance: Coping with Copper without Cooperativity? |
title_full | Bacterial Metal Resistance: Coping with Copper without Cooperativity? |
title_fullStr | Bacterial Metal Resistance: Coping with Copper without Cooperativity? |
title_full_unstemmed | Bacterial Metal Resistance: Coping with Copper without Cooperativity? |
title_short | Bacterial Metal Resistance: Coping with Copper without Cooperativity? |
title_sort | bacterial metal resistance: coping with copper without cooperativity? |
topic | Commentary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262934/ https://www.ncbi.nlm.nih.gov/pubmed/34126768 http://dx.doi.org/10.1128/mBio.00653-21 |
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