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Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs

Borrelia burgdorferi, the etiological agent of Lyme disease, persists in nature through an enzootic cycle consisting of a vertebrate host and an Ixodes tick vector. The sequence motifs modified by two well-characterized restriction/modification (R/M) loci of B. burgdorferi type strain B31 were recen...

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Autores principales: Wachter, Jenny, Martens, Craig, Barbian, Kent, Rego, Ryan O. M., Rosa, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262957/
https://www.ncbi.nlm.nih.gov/pubmed/34156261
http://dx.doi.org/10.1128/mBio.01288-21
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author Wachter, Jenny
Martens, Craig
Barbian, Kent
Rego, Ryan O. M.
Rosa, Patricia
author_facet Wachter, Jenny
Martens, Craig
Barbian, Kent
Rego, Ryan O. M.
Rosa, Patricia
author_sort Wachter, Jenny
collection PubMed
description Borrelia burgdorferi, the etiological agent of Lyme disease, persists in nature through an enzootic cycle consisting of a vertebrate host and an Ixodes tick vector. The sequence motifs modified by two well-characterized restriction/modification (R/M) loci of B. burgdorferi type strain B31 were recently described, but the methylation profiles of other Lyme disease Borrelia bacteria have not been characterized. Here, the methylomes of B. burgdorferi type strain B31 and 7 clonal derivatives, along with B. burgdorferi N40, B. burgdorferi 297, B. burgdorferi CA-11, B. afzelii PKo, B. afzelii BO23, and B. garinii PBr, were defined through PacBio single-molecule real-time (SMRT) sequencing. This analysis revealed 9 novel sequence motifs methylated by the plasmid-encoded restriction/modification enzymes of these Borrelia strains. Furthermore, while a previous analysis of B. burgdorferi B31 revealed an epigenetic impact of methylation on the global transcriptome, the current data contradict those findings; our analyses of wild-type B. burgdorferi B31 revealed no consistent differences in gene expression among isogenic derivatives lacking one or more restriction/modification enzymes.
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spelling pubmed-82629572021-07-23 Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs Wachter, Jenny Martens, Craig Barbian, Kent Rego, Ryan O. M. Rosa, Patricia mBio Research Article Borrelia burgdorferi, the etiological agent of Lyme disease, persists in nature through an enzootic cycle consisting of a vertebrate host and an Ixodes tick vector. The sequence motifs modified by two well-characterized restriction/modification (R/M) loci of B. burgdorferi type strain B31 were recently described, but the methylation profiles of other Lyme disease Borrelia bacteria have not been characterized. Here, the methylomes of B. burgdorferi type strain B31 and 7 clonal derivatives, along with B. burgdorferi N40, B. burgdorferi 297, B. burgdorferi CA-11, B. afzelii PKo, B. afzelii BO23, and B. garinii PBr, were defined through PacBio single-molecule real-time (SMRT) sequencing. This analysis revealed 9 novel sequence motifs methylated by the plasmid-encoded restriction/modification enzymes of these Borrelia strains. Furthermore, while a previous analysis of B. burgdorferi B31 revealed an epigenetic impact of methylation on the global transcriptome, the current data contradict those findings; our analyses of wild-type B. burgdorferi B31 revealed no consistent differences in gene expression among isogenic derivatives lacking one or more restriction/modification enzymes. American Society for Microbiology 2021-06-22 /pmc/articles/PMC8262957/ /pubmed/34156261 http://dx.doi.org/10.1128/mBio.01288-21 Text en https://doi.org/10.1128/AuthorWarrantyLicense.v1This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
spellingShingle Research Article
Wachter, Jenny
Martens, Craig
Barbian, Kent
Rego, Ryan O. M.
Rosa, Patricia
Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs
title Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs
title_full Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs
title_fullStr Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs
title_full_unstemmed Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs
title_short Epigenomic Landscape of Lyme Disease Spirochetes Reveals Novel Motifs
title_sort epigenomic landscape of lyme disease spirochetes reveals novel motifs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262957/
https://www.ncbi.nlm.nih.gov/pubmed/34156261
http://dx.doi.org/10.1128/mBio.01288-21
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