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Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers

In this article, we investigate patterns of microbial DNA contamination in targeted 16S rRNA amplicon sequencing (16S deep sequencing) and demonstrate how this can be used to filter background bacterial DNA in diagnostic microbiology. We also investigate the importance of sequencing depth. We first...

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Detalles Bibliográficos
Autores principales:	Dyrhovden, Ruben, Rippin, Martin, Øvrebø, Kjell Kåre, Nygaard, Randi M., Ulvestad, Elling, Kommedal, Øyvind
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262989/ https://www.ncbi.nlm.nih.gov/pubmed/34101489 http://dx.doi.org/10.1128/mBio.00598-21

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