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Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity
Albumin is abundant in serum but is also excreted at mucosal surfaces and enters tissues when inflammation increases vascular permeability. Host-associated opportunistic pathogens encounter albumin during commensalism and when causing infections. Considering the ubiquitous presence of albumin, we in...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262992/ https://www.ncbi.nlm.nih.gov/pubmed/34154403 http://dx.doi.org/10.1128/mBio.00531-21 |
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author | Austermeier, Sophie Pekmezović, Marina Porschitz, Pauline Lee, Sejeong Kichik, Nessim Moyes, David L. Ho, Jemima Kotowicz, Natalia K. Naglik, Julian R. Hube, Bernhard Gresnigt, Mark S. |
author_facet | Austermeier, Sophie Pekmezović, Marina Porschitz, Pauline Lee, Sejeong Kichik, Nessim Moyes, David L. Ho, Jemima Kotowicz, Natalia K. Naglik, Julian R. Hube, Bernhard Gresnigt, Mark S. |
author_sort | Austermeier, Sophie |
collection | PubMed |
description | Albumin is abundant in serum but is also excreted at mucosal surfaces and enters tissues when inflammation increases vascular permeability. Host-associated opportunistic pathogens encounter albumin during commensalism and when causing infections. Considering the ubiquitous presence of albumin, we investigated its role in the pathogenesis of infections with the model human fungal pathogen, Candida albicans. Albumin was introduced in various in vitro models that mimic different stages of systemic or mucosal candidiasis, where it reduced the ability of C. albicans to damage host cells. The amphipathic toxin candidalysin mediates necrotic host cell damage induced by C. albicans. Using cellular and biophysical assays, we determined that albumin functions by neutralizing candidalysin through hydrophobic interactions. We discovered that albumin, similarly, can neutralize a variety of fungal (α-amanitin), bacterial (streptolysin O and staurosporin), and insect (melittin) hydrophobic toxins. These data suggest albumin as a defense mechanism against toxins, which can play a role in the pathogenesis of microbial infections. |
format | Online Article Text |
id | pubmed-8262992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-82629922021-07-23 Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity Austermeier, Sophie Pekmezović, Marina Porschitz, Pauline Lee, Sejeong Kichik, Nessim Moyes, David L. Ho, Jemima Kotowicz, Natalia K. Naglik, Julian R. Hube, Bernhard Gresnigt, Mark S. mBio Research Article Albumin is abundant in serum but is also excreted at mucosal surfaces and enters tissues when inflammation increases vascular permeability. Host-associated opportunistic pathogens encounter albumin during commensalism and when causing infections. Considering the ubiquitous presence of albumin, we investigated its role in the pathogenesis of infections with the model human fungal pathogen, Candida albicans. Albumin was introduced in various in vitro models that mimic different stages of systemic or mucosal candidiasis, where it reduced the ability of C. albicans to damage host cells. The amphipathic toxin candidalysin mediates necrotic host cell damage induced by C. albicans. Using cellular and biophysical assays, we determined that albumin functions by neutralizing candidalysin through hydrophobic interactions. We discovered that albumin, similarly, can neutralize a variety of fungal (α-amanitin), bacterial (streptolysin O and staurosporin), and insect (melittin) hydrophobic toxins. These data suggest albumin as a defense mechanism against toxins, which can play a role in the pathogenesis of microbial infections. American Society for Microbiology 2021-06-22 /pmc/articles/PMC8262992/ /pubmed/34154403 http://dx.doi.org/10.1128/mBio.00531-21 Text en Copyright © 2021 Austermeier et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Austermeier, Sophie Pekmezović, Marina Porschitz, Pauline Lee, Sejeong Kichik, Nessim Moyes, David L. Ho, Jemima Kotowicz, Natalia K. Naglik, Julian R. Hube, Bernhard Gresnigt, Mark S. Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity |
title | Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity |
title_full | Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity |
title_fullStr | Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity |
title_full_unstemmed | Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity |
title_short | Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity |
title_sort | albumin neutralizes hydrophobic toxins and modulates candida albicans pathogenicity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262992/ https://www.ncbi.nlm.nih.gov/pubmed/34154403 http://dx.doi.org/10.1128/mBio.00531-21 |
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