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Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity

Albumin is abundant in serum but is also excreted at mucosal surfaces and enters tissues when inflammation increases vascular permeability. Host-associated opportunistic pathogens encounter albumin during commensalism and when causing infections. Considering the ubiquitous presence of albumin, we in...

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Autores principales: Austermeier, Sophie, Pekmezović, Marina, Porschitz, Pauline, Lee, Sejeong, Kichik, Nessim, Moyes, David L., Ho, Jemima, Kotowicz, Natalia K., Naglik, Julian R., Hube, Bernhard, Gresnigt, Mark S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262992/
https://www.ncbi.nlm.nih.gov/pubmed/34154403
http://dx.doi.org/10.1128/mBio.00531-21
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author Austermeier, Sophie
Pekmezović, Marina
Porschitz, Pauline
Lee, Sejeong
Kichik, Nessim
Moyes, David L.
Ho, Jemima
Kotowicz, Natalia K.
Naglik, Julian R.
Hube, Bernhard
Gresnigt, Mark S.
author_facet Austermeier, Sophie
Pekmezović, Marina
Porschitz, Pauline
Lee, Sejeong
Kichik, Nessim
Moyes, David L.
Ho, Jemima
Kotowicz, Natalia K.
Naglik, Julian R.
Hube, Bernhard
Gresnigt, Mark S.
author_sort Austermeier, Sophie
collection PubMed
description Albumin is abundant in serum but is also excreted at mucosal surfaces and enters tissues when inflammation increases vascular permeability. Host-associated opportunistic pathogens encounter albumin during commensalism and when causing infections. Considering the ubiquitous presence of albumin, we investigated its role in the pathogenesis of infections with the model human fungal pathogen, Candida albicans. Albumin was introduced in various in vitro models that mimic different stages of systemic or mucosal candidiasis, where it reduced the ability of C. albicans to damage host cells. The amphipathic toxin candidalysin mediates necrotic host cell damage induced by C. albicans. Using cellular and biophysical assays, we determined that albumin functions by neutralizing candidalysin through hydrophobic interactions. We discovered that albumin, similarly, can neutralize a variety of fungal (α-amanitin), bacterial (streptolysin O and staurosporin), and insect (melittin) hydrophobic toxins. These data suggest albumin as a defense mechanism against toxins, which can play a role in the pathogenesis of microbial infections.
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spelling pubmed-82629922021-07-23 Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity Austermeier, Sophie Pekmezović, Marina Porschitz, Pauline Lee, Sejeong Kichik, Nessim Moyes, David L. Ho, Jemima Kotowicz, Natalia K. Naglik, Julian R. Hube, Bernhard Gresnigt, Mark S. mBio Research Article Albumin is abundant in serum but is also excreted at mucosal surfaces and enters tissues when inflammation increases vascular permeability. Host-associated opportunistic pathogens encounter albumin during commensalism and when causing infections. Considering the ubiquitous presence of albumin, we investigated its role in the pathogenesis of infections with the model human fungal pathogen, Candida albicans. Albumin was introduced in various in vitro models that mimic different stages of systemic or mucosal candidiasis, where it reduced the ability of C. albicans to damage host cells. The amphipathic toxin candidalysin mediates necrotic host cell damage induced by C. albicans. Using cellular and biophysical assays, we determined that albumin functions by neutralizing candidalysin through hydrophobic interactions. We discovered that albumin, similarly, can neutralize a variety of fungal (α-amanitin), bacterial (streptolysin O and staurosporin), and insect (melittin) hydrophobic toxins. These data suggest albumin as a defense mechanism against toxins, which can play a role in the pathogenesis of microbial infections. American Society for Microbiology 2021-06-22 /pmc/articles/PMC8262992/ /pubmed/34154403 http://dx.doi.org/10.1128/mBio.00531-21 Text en Copyright © 2021 Austermeier et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Austermeier, Sophie
Pekmezović, Marina
Porschitz, Pauline
Lee, Sejeong
Kichik, Nessim
Moyes, David L.
Ho, Jemima
Kotowicz, Natalia K.
Naglik, Julian R.
Hube, Bernhard
Gresnigt, Mark S.
Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity
title Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity
title_full Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity
title_fullStr Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity
title_full_unstemmed Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity
title_short Albumin Neutralizes Hydrophobic Toxins and Modulates Candida albicans Pathogenicity
title_sort albumin neutralizes hydrophobic toxins and modulates candida albicans pathogenicity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262992/
https://www.ncbi.nlm.nih.gov/pubmed/34154403
http://dx.doi.org/10.1128/mBio.00531-21
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