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Loss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis

The bacterial flagellar motor is a complex macromolecular machine whose function and self-assembly present a fascinating puzzle for structural biologists. Here, we report that in diverse bacterial species, cell lysis leads to loss of the cytoplasmic switch complex and associated ATPase before other...

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Detalles Bibliográficos
Autores principales: Kaplan, Mohammed, Tocheva, Elitza I., Briegel, Ariane, Dobro, Megan J., Chang, Yi-Wei, Subramanian, Poorna, McDowall, Alasdair W., Beeby, Morgan, Jensen, Grant J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263016/
https://www.ncbi.nlm.nih.gov/pubmed/34098733
http://dx.doi.org/10.1128/mBio.00298-21
Descripción
Sumario:The bacterial flagellar motor is a complex macromolecular machine whose function and self-assembly present a fascinating puzzle for structural biologists. Here, we report that in diverse bacterial species, cell lysis leads to loss of the cytoplasmic switch complex and associated ATPase before other components of the motor. This loss may be prevented by the formation of a cytoplasmic vesicle around the complex. These observations suggest a relatively loose association of the switch complex with the rest of the flagellar machinery.