Cargando…

Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2

COVID-19 is a global crisis of unimagined dimensions. Currently, Remedesivir is only fully licensed FDA therapeutic. A major target of the vaccine effort is the SARS-CoV-2 spike-hACE2 interaction, and assessment of efficacy relies on time consuming neutralization assay. Here, we developed a cell fus...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhao, Min, Su, Pei-Yi, Castro, Danielle A., Tripler, Therese N., Hu, Yingxia, Cook, Matthew, Ko, Albert I., Farhadian, Shelli F., Israelow, Benjamin, Dela Cruz, Charles S., Xiong, Yong, Sutton, Richard E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263067/ https://www.ncbi.nlm.nih.gov/pubmed/34166473 http://dx.doi.org/10.1371/journal.ppat.1009683

Ejemplares similares

Development of an efficient reproducible cell-cell transmission assay for rapid quantification of SARS-CoV-2 spike interaction with hACE2
por: Ssenyange, George, et al.
Publicado: (2022)

Prediction of infectivity of SARS-CoV-2 virus based on Spike-hACE-2 interaction
por: Chaudhuri, Dwaipayan, et al.
Publicado: (2022)

Fatal Neurodissemination and SARS-CoV-2 Tropism in K18-hACE2 Mice Is Only Partially Dependent on hACE2 Expression
por: Carossino, Mariano, et al.
Publicado: (2022)

Identification and characterization of 7-azaindole derivatives as inhibitors of the SARS-CoV-2 spike-hACE2 protein interaction
por: Wang, Chaojie, et al.
Publicado: (2023)

Comparative pathology of the nasal epithelium in K18-hACE2 Tg mice, hACE2 Tg mice, and hamsters infected with SARS-CoV-2
por: Yu, Pin, et al.
Publicado: (2022)

The pH Effects on SARS-CoV and SARS-CoV-2 Spike Proteins in the Process of Binding to hACE2
por: Xie, Yixin, et al.
Publicado: (2021)

The pH Effects on SARS-CoV and SARS-CoV-2 Spike Proteins in the Process of Binding to hACE2
por: Xie, Yixin, et al.
Publicado: (2022)

Phage Display-Derived Compounds Displace hACE2 from Its Complex with SARS-CoV-2 Spike Protein
por: Sevenich, Marc, et al.
Publicado: (2022)

Identification of hACE2-interacting sites in SARS-CoV-2 spike receptor binding domain for antiviral drugs screening
por: Hu, Xiaopeng, et al.
Publicado: (2022)

DNA aptamers inhibit SARS-CoV-2 spike-protein binding to hACE2 by an RBD- independent or dependent approach
por: Silwal, Achut Prasad, et al.
Publicado: (2022)

Lucidenic acid A inhibits the binding of hACE2 receptor with spike protein to prevent SARS-CoV-2 invasion
por: Xu, Juan, et al.
Publicado: (2022)

Site Density Functional Theory and Structural Bioinformatics Analysis of the SARS-CoV Spike Protein and hACE2 Complex
por: Kumawat, Nitesh, et al.
Publicado: (2022)

Computational studies evidenced the potential of steroidal lactone to disrupt surface interaction of SARS-CoV-2 spike protein and hACE2
por: Yadav, Ajay, et al.
Publicado: (2022)

Design of hACE2-based small peptide inhibitors against spike protein of SARS-CoV-2: a computational approach
por: Dhingra, Naveen, et al.
Publicado: (2023)

Exploring the disruption of SARS-CoV-2 RBD binding to hACE2
por: Carter, Camryn, et al.
Publicado: (2023)

hACE2 Fc-neutralization antibody cocktail provides synergistic protection against SARS-CoV-2 and its spike RBD variants
por: Liu, Junli, et al.
Publicado: (2021)

Missense mutations in spike protein of SARS‐CoV‐2 delta variant contribute to the alteration in viral structure and interaction with hACE2 receptor
por: Mahmood, Tousif Bin, et al.
Publicado: (2022)

hACE2-Induced Allosteric Activation in SARS-CoV versus SARS-CoV-2 Spike Assemblies Revealed by Structural Dynamics
por: Chen, Chengbo, et al.
Publicado: (2023)

SARS-CoV-2 Spike Protein Intensifies Cerebrovascular Complications in Diabetic hACE2 Mice through RAAS and TLR Signaling Activation
por: Burnett, Faith N., et al.
Publicado: (2023)

Attractive and repulsive residue fragments at the interface of SARS-CoV-2 and hACE2
por: Rodriguez, Jorge H.
Publicado: (2021)

Therapeutic antibodies, targeting the SARS-CoV-2 spike N-terminal domain, protect lethally infected K18-hACE2 mice
por: Noy-Porat, Tal, et al.
Publicado: (2021)

An efficient system to generate truncated human angiotensin converting enzyme 2 (hACE2) capable of binding RBD and spike protein of SARS-CoV2
por: Gao, Xiangzheng, et al.
Publicado: (2021)

Altered hACE2 binding affinity and S1/S2 cleavage efficiency of SARS-CoV-2 spike protein mutants affect viral cell entry
por: Wang, Ke, et al.
Publicado: (2023)

Intranasal Liposomal Formulation of Spike Protein Adjuvanted with CpG Protects and Boosts Heterologous Immunity of hACE2 Transgenic Mice to SARS-CoV-2 Infection
por: Russo, Momtchilo, et al.
Publicado: (2023)

Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection
por: Rathnasinghe, Raveen, et al.
Publicado: (2020)

Comparison of transgenic and adenovirus hACE2 mouse models for SARS-CoV-2 infection
por: Rathnasinghe, Raveen, et al.
Publicado: (2020)

Effects of simeprevir on the replication of SARS-CoV-2 in vitro and in transgenic hACE2 mice
por: Muturi, Elishiba, et al.
Publicado: (2022)

Neuroinvasion and Neurotropism by SARS-CoV-2 Variants in the K18-hACE2 Mouse
por: Seehusen, Frauke, et al.
Publicado: (2022)

A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels
por: Alves, Juliano, et al.
Publicado: (2021)

Differential binding of SARS-CoV-2 Spike protein variants to its cognate receptor hACE2 using molecular modeling based binding analysis
por: Baig, Mirza Sarwar, et al.
Publicado: (2021)

Pathogenicity of severe acute respiratory coronavirus deletion mutants in hACE-2 transgenic mice
por: DeDiego, Marta L., et al.
Publicado: (2008)

COVID-19 Treatments and Pathogenesis Including Anosmia in K18-hACE2 mice
por: Zheng, Jian, et al.
Publicado: (2020)

Interaction Analysis of the Spike Protein of Delta and Omicron Variants of SARS-CoV-2 with hACE2 and Eight Monoclonal Antibodies Using the Fragment Molecular Orbital Method
por: Hwang, Sungbo, et al.
Publicado: (2022)

VSV-ΔG-Spike Candidate Vaccine Induces Protective Immunity and Protects K18-hACE2 Mice against SARS-CoV-2 Variants
por: Yahalom-Ronen, Yfat, et al.
Publicado: (2023)

Evaluation of K18-hACE2 Mice as a Model of SARS-CoV-2 Infection
por: Moreau, Gregory Brett, et al.
Publicado: (2020)

Genetic variability of human angiotensin‐converting enzyme 2 (hACE2) among various ethnic populations
por: Li, Quan, et al.
Publicado: (2020)

De novo design of potent and resilient hACE2 decoys to neutralize SARS-CoV-2
por: Linsky, Thomas W., et al.
Publicado: (2020)

The Infection and Pathogenicity of SARS-CoV-2 Variant B.1.351 in hACE2 Mice
por: Chen, Qi, et al.
Publicado: (2021)

The Tissue Distribution of SARS-CoV-2 in Transgenic Mice With Inducible Ubiquitous Expression of hACE2
por: Dolskiy, Alexander A., et al.
Publicado: (2022)

Chronological brain lesions after SARS-CoV-2 infection in hACE2-transgenic mice
por: Vidal, Enric, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_f5mq54urvrbcblvohec9thbl8i