Cargando…

Effect of Human or Mouse IL-7 on the Homeostasis of Porcine T Lymphocytes

Due to the inconsistent fluctuation of blood supply for transfusion, much attention has been paid to the development of artificial blood using other animals. Although mini-pigs are candidate animals, contamination of mini-pig T cells in artificial blood may cause a major safety concern. Therefore, i...

Descripción completa

Detalles Bibliográficos
Autores principales: Hong, Ji Hwa, Kim, Sang Hoon, Kim, Hyun Gyung, Jang, Jun Ho, Son, Ryeo Gang, Pack, Seung Pil, Park, Young-Ho, Kang, Philyong, Jeong, Kang-Jin, Kim, Ji-Su, Choi, Hanbyeul, Kim, Sun-Uk, Jung, Yong Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Association of Immunologists 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263216/
https://www.ncbi.nlm.nih.gov/pubmed/34277114
http://dx.doi.org/10.4110/in.2021.21.e24
Descripción
Sumario:Due to the inconsistent fluctuation of blood supply for transfusion, much attention has been paid to the development of artificial blood using other animals. Although mini-pigs are candidate animals, contamination of mini-pig T cells in artificial blood may cause a major safety concern. Therefore, it is important to analyze the cross-reactivity of IL-7, the major survival factor for T lymphocytes, between human, mouse, and mini-pig. Thus, we compared the protein sequences of IL-7 and found that porcine IL-7 was evolutionarily different from human IL-7. We also observed that when porcine T cells were cultured with either human or mouse IL-7, these cells did not increase the survival or proliferation compared to negative controls. These results suggest that porcine T cells do not recognize human or mouse IL-7 as their survival factor.